Expression of MIZ1<italic/> in peripheral blood mononuclear cells of patients with community-acquired pneumonia and its clinical significance_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

CAO Di , YIN Yudong , SHEN Hong , WEI Ming , FENG Shenghu , CHEN Xi , YANG Chunxia ,  GU Li

Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing Institute of Respiratory Medicine, Beijing 100020, P. R. China;

Corresponding author：

GU Li, Email: guli2013227@foxmail.com

Keywords：

Community-acquired pneumonia; Severity; Biomarker; Myc-interacting zinc finger protein-1

DOI：

10.7507/1671-6205.202101051

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Objective To measure the expression level of Myc-interacting zinc finger protein-1 (MIZ1) in peripheral blood mononuclear cells (PBMC) of patients with severe and non-severe community-acquired pneumonia (CAP) and its relationship with inflammatory factors. Methods Thirty-six CAP patients from Beijing Chaoyang Hospital from April 2018 to June 2019 were enrolled in this study. MIZ1 mRNA level in PBMC were measured by reverse transcriptase-quantitative polymerase chain reaction. The levels of interleukin (IL)-6, IL-8, IL-10, and interferon-α in the serum of patients were measured by enzyme-linked immunosorbent assay. The levels of MIZ1 mRNA and inflammatory factors were compared between the severe CAP patients and the non-severe CAP patients. Results Compared with non-severe CAP patients, the MIZ1 mRNA level in the PBMC of severe CAP patients was lower (P<0.05) than non-severe group. Receiver operating characteristic (ROC) curve of the expression level of MIZ1 in PBMC was calculated according to whether CAP was severe or non-severe, and the area under ROC curve was 0.731 (P=0.018). Spearman correlation analysis showed that MIZ1 mRNA was negatively correlated with IL-10 level in the severe CAP patients (Spearman correlation co-efficient was –0.620, P<0.05). Conclusions MIZ1 may indicate the severity of CAP. MIZ1 may affect IL-10 so as to play a role in inflammation regulation.

Citation： CAO Di, YIN Yudong, SHEN Hong, WEI Ming, FENG Shenghu, CHEN Xi, YANG Chunxia, GU Li. Expression of MIZ1 in peripheral blood mononuclear cells of patients with community-acquired pneumonia and its clinical significance. Chinese Journal of Respiratory and Critical Care Medicine, 2021, 20(11): 780-783. doi: 10.7507/1671-6205.202101051 Copy

1.	Chen L, Zhou F, Li H, et al. Disease characteristics and management of hospitalised adolescents and adults with community-acquired pneumonia in China: a retrospective multicentre survey. BMJ Open, 2018, 8(2): e018709.
2.	Torres A, Chalmers JD, Dela Cruz CS, et al. Challenges in severe community-acquired pneumonia: a point-of-view review. Intensive Care Med, 2019, 45(2): 159-171.
3.	Peukert K, Staller P, Schneider A, et al. An alternative pathway for gene regulation by Myc. EMBO J, 1997, 16(18): 5672-5686.
4.	Do-Umehara HC, Chen C, Urich D, et al. Suppression of inflammation and acute lung injury by Miz1 via repression of C/EBP-delta. Nat Immunol, 2013, 14(5): 461-469.
5.	Liu J, Zhao Y, Eilers M, et al. Miz1 is a signal- and pathway-specific modulator or regulator (SMOR) that suppresses TNF-alpha-induced JNK1 activation. Proc Natl Acad Sci U S A, 2009, 106(43): 18279-18284.
6.	武文姣. Miz1负性调控天然抗流感免疫中Ⅰ型干扰素表达的作用机制研究. 广州: 南方医科大学, 2019: 1-109.
7.	Chen P, Wang WY, Zhang YP, et al. Decreased MIZ1 expression in severe experimental acute pancreatitis: a rat study. Dig Dis Sci, 2016, 61(3): 758-766.
8.	Saba I, Kosan C, Vassen L, et al. Miz-1 is required to coordinate the expression of TCRbeta and p53 effector genes at the pre-TCR "beta-selection" checkpoint. J Immunol, 2011, 187(6): 2982-2992.
9.	Saito M, Novak U, Piovan E, et al. BCL6 suppression of BCL2 via Miz1 and its disruption in diffuse large B cell lymphoma. Proc Natl Acad Sci U S A, 2009, 106(27): 11294-11299.
10.	中华医学会呼吸病学分会. 中国成人社区获得性肺炎诊断和治疗指南(2016版). 中华结核和呼吸杂志, 2016, 39(4): 1-27.
11.	Metlay JP, Waterer GW, Long AC, et al. Diagnosis and Treatment of Adults with Community-acquired Pneumonia. An Official Clinical Practice Guideline of the American Thoracic Society and Infectious Diseases Society of America. Am J Respir Crit Care Med, 2019, 200(7): e45-e67.
12.	Zhang WJ, Zhangyuan GY, Wang F, et al. The zinc finger protein Miz1 suppresses liver tumorigenesis by restricting hepatocyte-driven macrophage activation and inflammation. Immunity, 2021, 54(6): 1168-1185.e8.
13.	Paats MS, Bergen IM, Hanselaar WE, et al. Local and systemic cytokine profiles in nonsevere and severe community-acquired pneumonia. Eur Respir J, 2013, 41(6): 1378-1385.

1. Chen L, Zhou F, Li H, et al. Disease characteristics and management of hospitalised adolescents and adults with community-acquired pneumonia in China: a retrospective multicentre survey. BMJ Open, 2018, 8(2): e018709.
2. Torres A, Chalmers JD, Dela Cruz CS, et al. Challenges in severe community-acquired pneumonia: a point-of-view review. Intensive Care Med, 2019, 45(2): 159-171.
3. Peukert K, Staller P, Schneider A, et al. An alternative pathway for gene regulation by Myc. EMBO J, 1997, 16(18): 5672-5686.
4. Do-Umehara HC, Chen C, Urich D, et al. Suppression of inflammation and acute lung injury by Miz1 via repression of C/EBP-delta. Nat Immunol, 2013, 14(5): 461-469.
5. Liu J, Zhao Y, Eilers M, et al. Miz1 is a signal- and pathway-specific modulator or regulator (SMOR) that suppresses TNF-alpha-induced JNK1 activation. Proc Natl Acad Sci U S A, 2009, 106(43): 18279-18284.
6. 武文姣. Miz1负性调控天然抗流感免疫中Ⅰ型干扰素表达的作用机制研究. 广州: 南方医科大学, 2019: 1-109.
7. Chen P, Wang WY, Zhang YP, et al. Decreased MIZ1 expression in severe experimental acute pancreatitis: a rat study. Dig Dis Sci, 2016, 61(3): 758-766.
8. Saba I, Kosan C, Vassen L, et al. Miz-1 is required to coordinate the expression of TCRbeta and p53 effector genes at the pre-TCR "beta-selection" checkpoint. J Immunol, 2011, 187(6): 2982-2992.
9. Saito M, Novak U, Piovan E, et al. BCL6 suppression of BCL2 via Miz1 and its disruption in diffuse large B cell lymphoma. Proc Natl Acad Sci U S A, 2009, 106(27): 11294-11299.
10. 中华医学会呼吸病学分会. 中国成人社区获得性肺炎诊断和治疗指南(2016版). 中华结核和呼吸杂志, 2016, 39(4): 1-27.
11. Metlay JP, Waterer GW, Long AC, et al. Diagnosis and Treatment of Adults with Community-acquired Pneumonia. An Official Clinical Practice Guideline of the American Thoracic Society and Infectious Diseases Society of America. Am J Respir Crit Care Med, 2019, 200(7): e45-e67.
12. Zhang WJ, Zhangyuan GY, Wang F, et al. The zinc finger protein Miz1 suppresses liver tumorigenesis by restricting hepatocyte-driven macrophage activation and inflammation. Immunity, 2021, 54(6): 1168-1185.e8.
13. Paats MS, Bergen IM, Hanselaar WE, et al. Local and systemic cytokine profiles in nonsevere and severe community-acquired pneumonia. Eur Respir J, 2013, 41(6): 1378-1385.

Chinese Journal of Respiratory and Critical Care Medicine

Expression of MIZ1 in peripheral blood mononuclear cells of patients with community-acquired pneumonia and its clinical significance

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