• 1. Research Core Facilities, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P. R. China;
  • 2. Precision Medicine Key Laboratory of Sichuan Province, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P. R. China;
SUN Huaiqiang, Email: sunhuaiqiang@scu.edu.cn
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Objective  To monitor the importance of establishing lung cancer models for immunological treatment through in vivo imaging system (IVIS). Methods  In this study, a new optical bioluminescence IVIS was used to confirm the tumour formation and luminescence in male BALB/c nude mice by injecting A549-luc cells. First, A549-luc cells which expressed luciferase stably were transferred into nude mice by tail vein injection in order to establish a stable and reliable model of lung cancer. Then, D-fluorescein potassium salt was intraperitoneally injected every other week. The tumor formation and growth were dynamically observed on day 7th, 14th and 21st by IVIS Spectrum and pathological exam with hematoxylin-eosin staining. Results  Animal model of lung cancer was successfully established, and the development of lung cancer was effectively monitored by IVIS real-time fluorescence value which was consistent with pathological exam, and tumor volume was correlated with fluorescence intensity (r=0.7996, P<0.01). Conclusions  IVIS has multiple benefits, including high sensitivity and specificity, simple operation, and no radiation. IVIS Spectrum can measure the fluorescence of tumor formed by injection of A549-luc cells in nude mice metastasis of lung cancer in a non-invasive, real-time and dynamic mode, which is worthy of promotion for using in clinical research.

Citation: CHEN Zhengju, GU Qiumei, XU Chunyan, LIU Zhiqiang, SHEN Meiling, SUN Huaiqiang. Application of in vivo imaging system to establish and evaluate an experimental mouse model of lung cancer. Chinese Journal of Respiratory and Critical Care Medicine, 2021, 20(11): 802-806. doi: 10.7507/1671-6205.202105060 Copy

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