Bioinformatics analysis of neutrophil gene expression profile in patients with acute respiratory disease syndrome_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

WANG Hanghang , QI Di , HE Jing , DENG Wang , WANG Daoxin ,  ZHAO Yan

Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P. R. China;

Corresponding author：

ZHAO Yan, Email: 304234@hospital.cqmu.edu.cn

Keywords：

Acute respiratory disease syndrome; Bioinformatics analysis; Hub gene

DOI：

10.7507/1671-6205.202107037

Video：

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Objective To explore the pathogenesis of acute respiratory disease syndrome (ARDS) by bioinformatics analysis of neutrophil gene expression profile in order to find new therapeutic targets. Methods The gene expression chips include ARDS patients and healthy volunteers were screened from the Gene Expression Omnibus (GEO) database. The differentially expressed genes were carried out through GEO2R, OmicsBean, STRING, and Cytoscape, then enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Gene and Genomes (KEGG) pathways was conducted to investigate the biological processes involved in ARDS via DAVID website. Results Bioinformatics analysis showed 86 differential genes achieved through the GEO2R website. Eighty-one genes were included in the STRING website for protein interaction analysis. The results of the interaction were further analyzed by Cytoscape software to obtain 11 hub genes: AHSP, ALAS2, CD177, CLEC4D, EPB42, GPR84, HBD, HVCN1, KLF1, SLC4A1, and STOM. GO analysis showed that the differential gene was enriched in the cellular component, especially the integrity of the plasma membrane. KEGG analysis showed that multiple pathways especially the cytokine receptor pathway involved in the pathogenesis of ARDS. Conclusions A variety of genes and pathways have been involved in the pathogenesis of ARDS. Eleven hub genes are screened, which may be involved in the pathogenesis of ARDS and can be used in subsequent studies.

Citation： WANG Hanghang, QI Di, HE Jing, DENG Wang, WANG Daoxin, ZHAO Yan. Bioinformatics analysis of neutrophil gene expression profile in patients with acute respiratory disease syndrome. Chinese Journal of Respiratory and Critical Care Medicine, 2021, 20(11): 789-794. doi: 10.7507/1671-6205.202107037 Copy

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15.	Montgomery MK, Osborne B, Brandon AE, et al. Regulation of mitochondrial metabolism in murine skeletal muscle by the medium-chain fatty acid receptor Gpr84. FASEB J, 2019, 33(11): 12264-12276.
16.	He J, Qi D, Wang DX, et al. Insulin upregulates the expression of epithelial sodium channel in vitro and in a mouse model of acute lung injury: role of mTORC2/SGK1 pathway. Exp Cell Res, 2015, 331(1): 164-175.
17.	O'Brien JM Jr, Phillips GS, Ali NA, et al. Body mass index is independently associated with hospital mortality in mechanically ventilated adults with acute lung injury. Crit Care Med, 2006, 34(3): 738-744.
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19.	Anzueto A, Frutos-Vivar F, Esteban A, et al. Influence of body mass index on outcome of the mechanically ventilated patients. Thorax, 2011, 66(1): 66-73.
20.	陈如杰, 林孟相, 庄荣, 等. 急性呼吸窘迫综合征预后的影响因素分析. 中华危重症医学杂志(电子版), 2013, 6(5): 30-34.

1. Meyer NJ, Calfee CS. Novel translational approaches to the search for precision therapies for acute respiratory distress syndrome. Lancet Respir Med, 2017, 5: 512-523.
2. 王行行, 王导新. NETs与急性呼吸窘迫综合征的研究进展. 基础医学与临床, 2021, 41(7): 1047-1051.
3. Juss JK, House D, Amour A, et al. Acute respiratory distress syndrome neutrophils have a distinct phenotype and are resistant to phosphoinositide 3-kinase inhibition. Am J Respir Crit Care Med, 2016, 194(8): 961-973.
4. Ito JT, Lourenço JD, Righetti RF, et al. Extracellular matrix component remodeling in respiratory diseases: what has been found in clinical and experimental studies?. Cells, 2019, 9(4): 342.
5. Lynn H, Sun X, Casanova N, et al. Genomic and genetic approaches to deciphering acute respiratory distress syndrome risk and mortality. Antioxid Redox Signal, 2019, 31(14): 1027-1052.
6. Bateman RM,. Sharpe MD, Jagger JE, et al. 36 th International Symposium on Intensive Care and Emergency Medicine:Brussels, Belgium. 15-18 March 2016. Crit Care, 2016, 20: 94.
7. Pu S, Wang D, Liu D, et al. Effect of sivelestat sodium in patients with acute lung injury or acute respiratory distress syndrome: a meta-analysis of randomized controlled trials. BMC Pulm Med, 2017, 17(1): 148.
8. Qi D, Tang X, He J, et al. Omentin protects against LPS-induced ARDS through suppressing pulmonary inflammation and promoting endothelial barrier via an Akt/eNOS-dependent mechanism. Cell Death Dis, 2016, 7(9): e2360.
9. Qi D, He J, Wang D, et al. 17beta-estradiol suppresses lipopolysaccharide-induced acute lung injury through PI3K/Akt/SGK1 mediated up-regulation of epithelial sodium channel (ENaC) in vivo and in vitro. Respir Res, 2014, 15(1): 159.
10. Zhu T, Li C, Zhang X, et al. GLP-1 analogue liraglutide enhances SP-A expression in LPS-induced acute lung injury through the TTF-1 signaling pathway. Mediators Inflamm, 2018, 2018: 3601454.
11. 张延威, 赵麦良, 张圣, 等. 血必净联合乌司他丁治疗脓毒症诱导急性呼吸窘迫综合征的效果观察. 临床荟萃, 2018, 33(7): 575-578.
12. Zhao Y, Zhang XY, Song ZX, et al. Rapamycin attenuates acute lung injury induced by LPS through inhibition of Th17 cell proliferation in mice. Sci Rep, 2016, 6: 20156.
13. Recio C, Lucy D, Iveson P, et al. The role of metabolite-sensing G protein-coupled peceptors in inflammation and metabolic sisease. Antioxid Redox Signal, 2018, 29(3): 237-256.
14. Recio C, Lucy D, Purvis GSD, et al. Activation of the immune-metabolic receptor GPR84 enhances inflammation and phagocytosis in macrophages. Front Immunol, 2018, 9: 1419.
15. Montgomery MK, Osborne B, Brandon AE, et al. Regulation of mitochondrial metabolism in murine skeletal muscle by the medium-chain fatty acid receptor Gpr84. FASEB J, 2019, 33(11): 12264-12276.
16. He J, Qi D, Wang DX, et al. Insulin upregulates the expression of epithelial sodium channel in vitro and in a mouse model of acute lung injury: role of mTORC2/SGK1 pathway. Exp Cell Res, 2015, 331(1): 164-175.
17. O'Brien JM Jr, Phillips GS, Ali NA, et al. Body mass index is independently associated with hospital mortality in mechanically ventilated adults with acute lung injury. Crit Care Med, 2006, 34(3): 738-744.
18. Gong MN, Bajwa EK, Thompson BT, et al. Body mass index is associated with the development of acute respiratory distress syndrome. Thorax, 2010, 65(1): 44-50.
19. Anzueto A, Frutos-Vivar F, Esteban A, et al. Influence of body mass index on outcome of the mechanically ventilated patients. Thorax, 2011, 66(1): 66-73.
20. 陈如杰, 林孟相, 庄荣, 等. 急性呼吸窘迫综合征预后的影响因素分析. 中华危重症医学杂志(电子版), 2013, 6(5): 30-34.

Chinese Journal of Respiratory and Critical Care Medicine

Bioinformatics analysis of neutrophil gene expression profile in patients with acute respiratory disease syndrome

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