Characteristics of intestinal flora in patients with allergic asthma_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LIU Ming ¹ , GUO Jie ¹ , ZHAO Dan ¹ ,  ZHANG Xuan ²

1. Department of Respiratory Mediciene, Hebei Traditional Chinese Medicine Hospital, Shijiazhuang, Hebei 050013, P. R. China;
2. Department of Respiratory and Critical Care Medicine, Hebei Traditional Chinese Medicine Hospital, Shijiazhuang, Hebei 050013, P. R. China;

Corresponding author：

ZHANG Xuan, Email: zhangxuan310227@163.com

Keywords：

Allergic asthma; intestinal flora; 16S rRNA

DOI：

10.7507/1671-6205.202304057

Video：

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Objective To analyze the characteristics of intestinal flora in patients with allergic asthma, so as to provide a theoretical basis for the development of new clinical treatment methods. Methods Fecal samples were collected from 14 patients with allergic asthma and 15 healthy people between January 2021 and December 2021, and 16S rRNA was used to analyze the composition and diversity of intestinal flora of the participants. Results There was no statistically significant difference in age, gender, BMI, or smoking history between the allergic asthma group and the control group (all P>0.05). Alpha diversity results showed that there was significant difference in the abundance of intestinal flora between the two groups, but there was no significant difference in the diversity of intestinal flora between the two groups. The results of β diversity analysis indicated that there were significant differences in the composition of bacterial flora between the allergic asthma group and the control group. The difference bacteria between the two groups at the genus level are Faecalibacterium, Roseburia, Alistipes, Sphingomonas, Dorea, Ruminococcaceae_UCG-002, Streptomyces, [Eubacterium]_venturiosum_group, Butyriococcus and Agathobacter. Conclusion Compared with healthy individuals, patients with allergic asthma have undergone significant changes in the composition of their gut microbiota, with various differential bacteria present. Among them, Roseburia and Eubacterium may be involved in the pathogenesis of allergic asthma through changes in short chain fatty acids.

Citation： LIU Ming, GUO Jie, ZHAO Dan, ZHANG Xuan. Characteristics of intestinal flora in patients with allergic asthma. Chinese Journal of Respiratory and Critical Care Medicine, 2024, 23(1): 1-6. doi: 10.7507/1671-6205.202304057 Copy

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33.	杨玉婷, 倪吉祥, 徐彪, 等. 肠道菌群通过短链脂肪酸参与过敏性哮喘发病的相关机制研究进展. 山东医药, 2021, 61(23): 4.

1. GBD 2015 Chronic Respiratory Disease Collaborators. Global, regional, and national deaths, prevalence, disability-adjusted life years, and years lived with disability for chronic obstructive pulmonary disease and asthma, 1990-2015: a systematic analysis for the Global Burden of Disease Study 2015. Lancet Respir Med, 2017, 5(9): 691-706.
2. Strachan DP. Hay fever, hygiene, and household size. BMJ, 1989, 299(6710): 1259-1260.
3. Björkstén B. The hygiene hypothesis: do we still believe in it?. Nestle Nutr Workshop Ser Pediatr Program, 2009, 64: 11-22, 251-257.
4. Couzin-Frankel J. Bacteria and asthma: untangling the links. Science, 2010, 330(6008): 1168-1169.
5. Magnus MC, Håberg SE, Stigum H, et al. Delivery by Cesarean section and early childhood respiratory symptoms and disorders: the Norwegian mother and child cohort study. Am J Epidemiol, 2011, 174(11): 1275-1285.
6. Schroeder BO, Bäckhed F. Signals from the gut microbiota to distant organs in physiology and disease. Nat Med, 2016, 22(10): 1079-1089.
7. Zhernakova A, Kurilshikov A, Bonder MJ, et al. Population-based metagenomics analysis reveals markers for gut microbiome composition and diversity. Science, 2016, 352(6285): 565-569.
8. Gritz EC, Bhandari V. The human neonatal gut microbiome: a brief review. Front Pediatr, 2015, 3: 17.
9. Yatsunenko T, Rey FE, Manary MJ, et al. Human gut microbiome viewed across age and geography. Nature, 2012, 486(7402): 222-227.
10. Marchesi JR, Adams DH, Fava F, et al. The gut microbiota and host health: a new clinical frontier. Gut, 2016, 65(2): 330-339.
11. Honda K, Littman DR. The microbiota in adaptive immune homeostasis and disease. Nature, 2016, 535(7610): 75-84.
12. Lawley TD, Walker AW. Intestinal colonization resistance. Immunology, 2013, 138(1): 1-11.
13. Maslowski KM, Vieira AT, Ng A, et al. Regulation of inflammatory responses by gut microbiota and chemoattractant receptor GPR43. Nature, 2009, 461(7268): 1282-1286.
14. Furusawa Y, Obata Y, Fukuda S, et al. Commensal microbe-derived butyrate induces the differentiation of colonic regulatory T cells. Nature, 2013, 504(7480): 446-450.
15. Hall AB, Tolonen AC, Xavier RJ. Human genetic variation and the gut microbiome in disease. Nat Rev Genet, 2017, 18(11): 690-699.
16. Turnbaugh PJ, Ley RE, Mahowald MA, et al. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature, 2006, 444(7122): 1027-1031.
17. Ridaura VK, Faith JJ, Rey FE, et al. Gut microbiota from twins discordant for obesity modulate metabolism in mice. Science, 2013, 341(6150): 1241214.
18. Smith MI, Yatsunenko T, Manary MJ, et al. Gut microbiomes of Malawian twin pairs discordant for kwashiorkor. Science, 2013, 339(6119): 548-554.
19. Mullish BH, Williams HR. Clostridium difficile infection and antibiotic-associated diarrhoea. Clin Med (Lond), 2018, 18(3): 237-241.
20. Cekanaviciute E, Yoo BB, Runia TF, et al. Gut bacteria from multiple sclerosis patients modulate human T cells and exacerbate symptoms in mouse models. Proc Natl Acad Sci U S A, 2017, 114(40): 10713-10718.
21. Tang WH, Wang Z, Levison BS, et al. Intestinal microbial metabolism of phosphatidylcholine and cardiovascular risk. N Engl J Med, 2013, 368(17): 1575-1584.
22. DeVries A, McCauley K, Fadrosh D, et al. Maternal prenatal immunity, neonatal trained immunity, and early airway microbiota shape childhood asthma development. Allergy, 2022, 77(12): 3617-3628.
23. Lynch SV, Pedersen O. The human intestinal microbiome in health and disease. N Engl J Med, 2016, 375(24): 2369-2379.
24. Budden KF, Gellatly SL, Wood DL, et al. Emerging pathogenic links between microbiota and the gut-lung axis. Nat Rev Microbiol, 2017, 15(1): 55-63.
25. Marsland BJ, Trompette A, Gollwitzer ES. The gut-lung axis in respiratory disease. Ann Am Thorac Soc, 2015, 12 Suppl 2: S150--S156.
26. Lee-Sarwar K, Dedrick S, Momeni B, et al. Association of the gut microbiome and metabolome with wheeze frequency in childhood asthma. J Allergy Clin Immunol, 2022, 150(2): 325-336.
27. Hufnagl K, Pali-Schöll I, Roth-Walter F, et al. Dysbiosis of the gut and lung microbiome has a role in asthma. Semin Immunopathol, 2020, 42(1): 75-93.
28. Kumari R, Ahuja V, Paul J. Fluctuations in butyrate-producing bacteria in ulcerative colitis patients of North India. World J Gastroenterol, 2013, 19(22): 3404-3414.
29. Machiels K, Sabino J, Vandermosten L, et al. Specific members of the predominant gut microbiota predict pouchitis following colectomy and IPAA in UC. Gut, 2017, 66(1): 79-88.
30. Vermeiren J, Van den Abbeele P, Laukens D, et al. Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment. FEMS Microbiol Ecol, 2012, 79(3): 685-696.
31. Mukherjee A, Lordan C, Ross RP, et al. Gut microbes from the phylogenetically diverse genus Eubacterium and their various contributions to gut health. Gut Microbes, 2020, 12(1): 1802866.
32. Verhaar BJH, Hendriksen HMA, de Leeuw FA, et al. Gut microbiota composition is related to AD pathology. Front Immunol, 2022, 12: 794519.
33. 杨玉婷, 倪吉祥, 徐彪, 等. 肠道菌群通过短链脂肪酸参与过敏性哮喘发病的相关机制研究进展. 山东医药, 2021, 61(23): 4.

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