Present Situation and Progress in Research on HIV/AIDS Laboratory Testing_Chinese Journal of Evidence-Based Medicine

Authors：

 BAI Lang , LEI Bingjun

Infectious Disease Center of West China Hospital of Sichuan University, Chengdu 610041, China;

Corresponding author：

BAI Lang, Email: pangbailang@163.com

Keywords：

Acquired Immunodeficiency Syndrome; Human immunodeficiency virus; Laboratory testing

DOI：

10.7507/1672-2531.20080045

Video：

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Acquired Immunodeficiency Syndrome (AIDS) is a severe infectious disease induced by human immuno deficiency virus (HIV). Laboratory testing plays an important role in the diagnosis of HIV/AIDS. In general, laboratory testing includes detection of virus antibodies and antigens, virus RNA, immune cells (CD4+, CD8+) and anti-HIV drug resistance. During the past twenty years, great progress has been made in laboratory testing. As research on HIV/AIDS has advanced and biotechnology has developed rapidly, different methods of testing have been discovered. In recent years, the application of molecular biotechnology and immunology has led to important advances for epidemiological surveys, clinical diagnosis and treatment of HIV. The existence of a testing method with high sensitivity and specificity is not only helpful for early diagnosis and prediction, monitoring and evaluation of therapeutic efficacy, but can also reduce the risk of false-negative results. HIV laboratory testing is now developing towards a simple, rapid, sensitive, accurate and automatic way of diagnosing this condition.

Citation： BAI Lang,LEI Bingjun. Present Situation and Progress in Research on HIV/AIDS Laboratory Testing. Chinese Journal of Evidence-Based Medicine, 2008, 08(3): 206-209. doi: 10.7507/1672-2531.20080045 Copy

1.	Mylonakis E, Paliou M, Lally M, et al. Laboratory testing for infection with the human immunodeficiency virus:established and novel approaches. Am J Med, 2000, 109(7): 568-576.
2.	Gurtler L, Muhlbacher A, Michl U, et al. Reduction of the diagnostic window with a new combined p24 antigen and human immunodeficiency virus antibody screening assay. J Virol Methods, 1998, 75(1): 27-38.
3.	Weber B, Berger A, Rabenau H, et al. Evaluation of a New Combined Antigen and Antibody Human Immunodeficiency Virus Screening Assay ,VIDAS HIV DUO Ultra. J Clin Microbiol, 2002, 40(4): 1420-1426.
4.	Weber B, Gürtler L, Thorstensson R, et al. Multicenter Evaluation of a New Automated Fourth-generation Human Immunodeficiency Virus Screening Assay with a sensitive Antigen Detection Module and High Specificity. J Clin Microbiol, 2002, 40(6): 1938-1946.
5.	Gurtler LG, Eberle J, Lorbeer B, et al. Sensitivity and specificity of commercial ELISA kits for screening anti-LAV/HTLV III. J Virol Methods, 1987, 15(1): 11-23.
6.	Petricciani JC, Epstein JS. The effects of the AIDS epidemic on the safety of the nation’s blood supply. Public Health Rep, 1988, 103(3): 236-241.
7.	Barbe F, Klein M, Badonnel Y. Early detection of antibodies to human immunodeficiency virus 1 by a third-generation enzyme immunoassay.A comparative study with the results of second-generation immunoassays and western blot. Ann Biol Clin(Paris), 1994, 52(5): 341-345.
8.	Suligoi B, Galli C, Massi M, et al. Precision and Accuracy of a Procedure for Detecting Recent Human Immunodeficiency Virus Infections by Calculating the Antibody Avidity Index by an Automated Immunoassay-Based Method. J Clin. Microbiol, 2002, 40(11): 4015-4020.
9.	Ly TD, Laperche S, Brennan C, et al. Evaluation of the sensitivity and specificity of six HIV combined p24 antigen and antibody assays. J Virol Methods, 2004, 122(2): 185-194.
10.	Bernard M Branson, MD. Rapid Test for HIV Antiboby. AIDS Reviews, 2000, 2: 76-83.
11.	魏经新. 免疫层析试验检测抗HIV1+2的临床应用价值. 中国艾滋病性病, 2003, 9(5): 280-281.
12.	郑晓丽. HIV检测研究进展与发展趋势. 现代检验医学杂志, 2004, 19(6): 64-67.
13.	冯晓英, 徐晓琴. 吸毒人群尿液血液标本HIV-1抗体ELISA检测对比分析. 中国性病艾滋病, 2003, 9(3): 132-133.
14.	赵立庆, 雷素萍, 李连学, 等. ELISA法检测尿液中HIV-1抗体的研究分析. 中国艾滋病性病, 2003, 9(5): 276-277.
15.	Nguyen KA, Busch MP. Evolving strategies for diagnosing human immunodeficiency virus infection. Am J Med, 2000, 109(7): 595-597.
16.	Celum CL, Coombs RW, Jones M, et al. Risk factors for repeatedly reactive HIV-1 EIA and indeterminate western blots. A population-based case-control study. Arch Intern Med, 1994, 154(5): 1129-1137.
17.	Riddler S, Mellors JW. Clinical use of Viral Load Measures. MMWR, April,1998.
18.	肖建春, 郑启唐, 赵之伟. HIV p24抗原检测技术的研究进展. 国外医学病毒学分册, 2003, 10(4): 111-114.
19.	魏民. HIV实验室检测研究进展和发展趋势. 国外医学病毒学分册, 2003, 10(3): 65-69.
20.	Mellors JW, Rinaldo CR, Gupta P, et al. Prognosis in HIV-1 Infection Predicted by the Quantity of virus in plasma. Science, 1996, 272(5): 1167.
21.	Lindback S, Karlsson AC, Mittler J, et al. Viral dynamics in primary HIV-1 infection. AIDS, 2000, 14(15): 2283-2291.
22.	Mellors JW, Munoz A, Giorgi JV, et al. Plasma Viral Load and CD4+ Lymphocytes as Prognostic Markers of HIV-1 Infection. Ann Intern Med, 1997, 126: 946-954.
23.	Abravaya K, Esping C, Hoenle R, et al. Performance of a Multiplex Qualitative PCR LCx Assay for Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Group M Subtypes, Group O, and HIV-2. J Clin Microbiol, 2000, 38(2): 716-723.
24.	Zhao YQ, Min Y, Miller JW, et al. Quantification of Human Immundeficiency virus type 1 Proviral DNA by Using TaqMan Technology. J Chin Microbiol, 2002, 40(2): 675-678.
25.	Leroy V, Montcho C, Manigart O, et al. Maternal plasma viral load, zidovudine and mother-to-child transmission of HIV-1 in Africa:DITRAME ANRS 049a trial. AIDS, 2001, 15(4): 517-522.
26.	Deeks S, Beatty G, Cohen PT, et al. Viral load and CD4+T cells changes in patients failing potent protease inhibitor therapy. Presented at the 5th Conference on Retroviruses and Opportunistic Infections; February 1-5, 1998; Chicago. Abstract 419.
27.	肖瑶, 张可, 裴丽健, 等. 中国HIV/ AIDS 患者CD4+ CD8+T淋巴细胞与外周血各组份间关系的研究. 中国艾滋病性病, 2004, 10(2): 83-88.
28.	Edelstein RE, Nickerson DA, Tobe VO, et al. Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine. J Clin Microbiol, 1998, 36(7): 569-572.

1. Mylonakis E, Paliou M, Lally M, et al. Laboratory testing for infection with the human immunodeficiency virus:established and novel approaches. Am J Med, 2000, 109(7): 568-576.
2. Gurtler L, Muhlbacher A, Michl U, et al. Reduction of the diagnostic window with a new combined p24 antigen and human immunodeficiency virus antibody screening assay. J Virol Methods, 1998, 75(1): 27-38.
3. Weber B, Berger A, Rabenau H, et al. Evaluation of a New Combined Antigen and Antibody Human Immunodeficiency Virus Screening Assay ,VIDAS HIV DUO Ultra. J Clin Microbiol, 2002, 40(4): 1420-1426.
4. Weber B, Gürtler L, Thorstensson R, et al. Multicenter Evaluation of a New Automated Fourth-generation Human Immunodeficiency Virus Screening Assay with a sensitive Antigen Detection Module and High Specificity. J Clin Microbiol, 2002, 40(6): 1938-1946.
5. Gurtler LG, Eberle J, Lorbeer B, et al. Sensitivity and specificity of commercial ELISA kits for screening anti-LAV/HTLV III. J Virol Methods, 1987, 15(1): 11-23.
6. Petricciani JC, Epstein JS. The effects of the AIDS epidemic on the safety of the nation’s blood supply. Public Health Rep, 1988, 103(3): 236-241.
7. Barbe F, Klein M, Badonnel Y. Early detection of antibodies to human immunodeficiency virus 1 by a third-generation enzyme immunoassay.A comparative study with the results of second-generation immunoassays and western blot. Ann Biol Clin(Paris), 1994, 52(5): 341-345.
8. Suligoi B, Galli C, Massi M, et al. Precision and Accuracy of a Procedure for Detecting Recent Human Immunodeficiency Virus Infections by Calculating the Antibody Avidity Index by an Automated Immunoassay-Based Method. J Clin. Microbiol, 2002, 40(11): 4015-4020.
9. Ly TD, Laperche S, Brennan C, et al. Evaluation of the sensitivity and specificity of six HIV combined p24 antigen and antibody assays. J Virol Methods, 2004, 122(2): 185-194.
10. Bernard M Branson, MD. Rapid Test for HIV Antiboby. AIDS Reviews, 2000, 2: 76-83.
11. 魏经新. 免疫层析试验检测抗HIV1+2的临床应用价值. 中国艾滋病性病, 2003, 9(5): 280-281.
12. 郑晓丽. HIV检测研究进展与发展趋势. 现代检验医学杂志, 2004, 19(6): 64-67.
13. 冯晓英, 徐晓琴. 吸毒人群尿液血液标本HIV-1抗体ELISA检测对比分析. 中国性病艾滋病, 2003, 9(3): 132-133.
14. 赵立庆, 雷素萍, 李连学, 等. ELISA法检测尿液中HIV-1抗体的研究分析. 中国艾滋病性病, 2003, 9(5): 276-277.
15. Nguyen KA, Busch MP. Evolving strategies for diagnosing human immunodeficiency virus infection. Am J Med, 2000, 109(7): 595-597.
16. Celum CL, Coombs RW, Jones M, et al. Risk factors for repeatedly reactive HIV-1 EIA and indeterminate western blots. A population-based case-control study. Arch Intern Med, 1994, 154(5): 1129-1137.
17. Riddler S, Mellors JW. Clinical use of Viral Load Measures. MMWR, April,1998.
18. 肖建春, 郑启唐, 赵之伟. HIV p24抗原检测技术的研究进展. 国外医学病毒学分册, 2003, 10(4): 111-114.
19. 魏民. HIV实验室检测研究进展和发展趋势. 国外医学病毒学分册, 2003, 10(3): 65-69.
20. Mellors JW, Rinaldo CR, Gupta P, et al. Prognosis in HIV-1 Infection Predicted by the Quantity of virus in plasma. Science, 1996, 272(5): 1167.
21. Lindback S, Karlsson AC, Mittler J, et al. Viral dynamics in primary HIV-1 infection. AIDS, 2000, 14(15): 2283-2291.
22. Mellors JW, Munoz A, Giorgi JV, et al. Plasma Viral Load and CD4+ Lymphocytes as Prognostic Markers of HIV-1 Infection. Ann Intern Med, 1997, 126: 946-954.
23. Abravaya K, Esping C, Hoenle R, et al. Performance of a Multiplex Qualitative PCR LCx Assay for Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Group M Subtypes, Group O, and HIV-2. J Clin Microbiol, 2000, 38(2): 716-723.
24. Zhao YQ, Min Y, Miller JW, et al. Quantification of Human Immundeficiency virus type 1 Proviral DNA by Using TaqMan Technology. J Chin Microbiol, 2002, 40(2): 675-678.
25. Leroy V, Montcho C, Manigart O, et al. Maternal plasma viral load, zidovudine and mother-to-child transmission of HIV-1 in Africa:DITRAME ANRS 049a trial. AIDS, 2001, 15(4): 517-522.
26. Deeks S, Beatty G, Cohen PT, et al. Viral load and CD4+T cells changes in patients failing potent protease inhibitor therapy. Presented at the 5th Conference on Retroviruses and Opportunistic Infections; February 1-5, 1998; Chicago. Abstract 419.
27. 肖瑶, 张可, 裴丽健, 等. 中国HIV/ AIDS 患者CD4+ CD8+T淋巴细胞与外周血各组份间关系的研究. 中国艾滋病性病, 2004, 10(2): 83-88.
28. Edelstein RE, Nickerson DA, Tobe VO, et al. Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine. J Clin Microbiol, 1998, 36(7): 569-572.

Chinese Journal of Evidence-Based Medicine

Present Situation and Progress in Research on HIV/AIDS Laboratory Testing

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