Diagnostic Value of LiPA and Phage-Based Assays for Rifampicin Resistance in Mycobacterium Tuberculosis: A Systematic Review_Chinese Journal of Evidence-Based Medicine

Authors：

QIU Jie ¹ , ZHANG Junyu ¹ , ZHANG Peizhen ² , ZHANG Ying ³ , MA Bin ⁴ ,  YANG Kehu ⁴

1. Women and Children Hospital of Gnasu Province, Lanzhou 730000, China2. Women and Children Hospital of Lanzhou City,Lanzhou 730000 China3. Ttuberculosis Rresearch Ccenter of Lanzhou University, Lanzhou 730000 China4. Evidence-Based Medicine Center of Lanzhou University, Lanzhou 730000 China;

Corresponding author：

YANG Kehu, Email: yangkh@lzu.edu.cn

Keywords：

Mycobacterium tuberculosis; Systematic review; SROC

DOI：

10.7507/1672-2531.20080147

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective 　To evaluate the diagnostic accuracy of LiPA and phage-based assays in detecting rifampicin resistance in Mycobacterium tuberculosis.
Methods 　A fully recursive literature search was conducted in PUBMED, EMBASE, CBMWeb, CSJD and CJFD. QUADAS items were used to evaluate the quality of the included studies. Meta-disc software was used to handle data from the included studies. SEN, SPE and SROC were used to assess the diagnostic accuracy of every individual diagnostic test.
Results 　A total of 42 studies were included finally. (1) LiPA for detection of rifampicin resistant Mycobacterium tuberculosis: 7 studies took BACTEC 460 assay as the reference test, and meta-analysis showed that the summary SEN = 0.98, summary SPE = 0.98, SROC (AUC) = 0.9924; 6 studies chose proportion assay as the reference test, and meta-analysis showed that the summary SEN = 0.97, summary SPE = 1.00, SROC (AUC) = 0.9961; and 3 studies took both BACTEC 460 assay and proportion assay as the reference tests, and meta-analysis showed that the summary SEN = 0.92, summary SPE = 0.98, SROC (AUC) = 0.9842. (2)Seven studies detected the rifampicin resistant Mycobacterium tuberculosis using Phage amplification assays (Commercial), taking BACTEC 460 assay and proportion assay as the reference tests. Meta-analysis showed that the summary SEN = 0.95, summary SPE = 0.95, SROC (AUC) = 0.9842. (3) Seven studies detected the rifampicin resistant Mycobacterium tuberculosis using Phage amplification assays (in-house), taking BACTEC 460 assay, proportion assay and absolute concentration as the reference tests. Meta-analysis showed that the summary SEN = 0.98, summary SPE = 0.98, SROC (AUC) = 0.9949. (4)Seven studies detected the rifampicin resistant Mycobacterium tuberculosis using Luciferase reporter phage assays (In-house), taking BACTEC 460 assay, proportion assay and absolute concentration as the reference tests. Meta-analysis showed that the summary SEN = 0.98, summary SPE = 0.98, SROC (AUC) = 0.9788.
Conclusion 　Current research confirms that Phage assay is a highly sensitive and specific test for the detection of rifampicin resistance in culture isolates and has a potential in improving the diagnostic accuracy of all diagnostic tests in detecting the rifampicin resistant Mycobacterium tuberculosis. LiPA is also a highly sensitive and specific test for the detection of rifampicin resistance, but the sensitivity appears to relatively decrease when it was used directly on clinical specimens. The results mentioned above need to be further confirmed by more high-quality studies.

Citation： QIU Jie,ZHANG Junyu,ZHANG Peizhen,ZHANG Ying,MA Bin,YANG Kehu. Diagnostic Value of LiPA and Phage-Based Assays for Rifampicin Resistance in Mycobacterium Tuberculosis: A Systematic Review. Chinese Journal of Evidence-Based Medicine, 2008, 08(8): 642-651. doi: 10.7507/1672-2531.20080147 Copy

1.	The WHO/IUATLD Global Project on Anti-Tuberculosis Drug Resistance Surveillance: Anti-Tuberculosis drug resistance in the world, report number 2 Volume. Geneva; 2004.
2.	王巍. 耐多药结核病的现状及诊断、处理对策. 军医进修学院学报, 2006, 27(6): 471-471.
3.	全国结核病流行病学抽样调查技术指导组. 第三次全国全国结核病流行病学抽样调查报告. 中华结核与呼吸杂志, 1992, 15(2): 69271.
4.	Fisher M. Diagnosis of MDR-TB: a developing world problem on a developed world budget. Expert Rev Mol Diagn, 2002, 2(2): 151-159.
5.	Parsons LM, Somoskovi A, Urbanczik R, et al. Laboratory diagnostic aspects of drug resistant tuberculosis. Front Biosci, 2004, 9: 2086-2105.
6.	Hirano K, Abe C, Takahashi M. Mutations in the rpo B gene of rifampin-resistant Mycobacterium tuberculosis strains isolated mostly in Asian countries and their rapid detection by line probe assay. J Clin Microbiol, 1999, 37: 2663-2666.
7.	Heifets LB, Cangelosi GA. Drug susceptibility testing of Mycobacterium tuberculosis: a neglected problem at the turn of the century. Int J Tuberc Lung Dis, 1999, 3(7): 564–581.
8.	Cavusoglu C, Hilmioglu S, Guneri S, et al. Characterization of rpo B mutations in Rifampin-resistant clinical isolates of Mycobacterium tuberculosis from Turkey by DNA sequencing and line probe assay. J Clin Microbiol, 2002, 40: 4435-4438.
9.	Hazbon MH. Recent advances in molecular methods for early diagnosis of tuberculosis and drug-resistant tuberculosis. Biomedical, 2004, 24(Suppl 1): 149-162.
10.	Somoskovi A, Parsons LM, Salfinger M. The molecular basis of resistance to isoniazid, rifampin, and pyrazinamide in Mycobacterium tuberculosis. Respir Res, 2001, 2(3): 164-168.
11.	Ahmad S, Mokaddas E, Fares E. Characterization of rpo B mutationsin rifampin-resistant clinical Mycobacterium tuberculosis isolates from Kuwait and Dubai. Diagn Microbiol Infect Dis, 2002, 44: 245-252.
12.	Rossau R, Traore H, De Beenhouwer H, et al. Evaluation of the INNO-LiPA Rif. TB assay, areverse hybridization assay for the simultaneous detection of Mycobacterium tuberculosis complex and its resistance to rifampin. Antimicrob Agents Chemother, 1997, 41: 2093-2098.
13.	Watterson S, Wilson S, Yates M, et al. Comparison of three molecular assays for rapid detection of rifampin resistanc in Mycobacterium tuberculosis. J Clin Microbiol, 1998, 36: 1969-1973.
14.	Whiting P, Rutjes AW, Reitsma JB, et al. The development of QUADAS: a tool for the quality assessment of studies of diagnostic accuracy included in systematic reviews. BMC Med Res Methodol, 2003, 3: 25.
15.	Gamboa F, Cardona PJ, Manterola JM, et al. Evaluation of a commercial probe assay fordetection of rifampin resistance in Mycobacterium tuberculosis directly from respiratory and nonrespiratory clinical samples. Eur J Clin Microbiol Infect Dis, 1998, 17: 189-192.
16.	Johansen I, Lundgren B, Sosnovskaja A, et al. Direct detection of multidrug-resistant Mycobacterium tuberculosis in clinical specimens in low- and high-incidence countries by line probe assay. J Clin Microbiol, 2003, 41: 4454-4456.
17.	Jureen P, Werngren J, Hoffner S. Evaluation of the line probe assay (LiPA) for rapid detection of Rifampicin resistance in Mycobac- terium tuberculosis. Tuberculosis, 2004, 84: 311-316.
18.	Sintchenko V, Chew W, Jelfs P, et al. Mutations in rpo B gene and rifabutin susceptibility of multidrug-resistant Mycobacterium tuberculosis strains isolated in Australia. Pathology, 1999, 31: 257-260.
19.	Tracevska T, Jansone I, Broka L, et al. Mutations in the rpo B and katG genes leading to drug resistance in Mycobacterium tuberculosis in Latvia. J Clin Microbiol, 2002, 40: 3789-3792.
20.	Johansen I, Lundgren B, Sosnovskaja A, et al. Direct detection of multidrug-resistant Mycobacterium tuberculosis in clinical specimens in low- and high-incidence countries by line probe assay. J Clin Microbiol, 2003, 41: 4454-4456.
21.	Viveiros M, Leandro C, Rodrigues L, et al. Direct application of the INNO-LiPA Rif.TB line-probe assay for rapid identification of Mycobacterium tuberculosis complex strains and detection of rifampin resistance in 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis. J Clin Microbiol, 2005, 43(9): 4880-4884.
22.	De Oliveira M, Rocha A, Oelemann M, et al. Rapid detection of resistance against Rifampicin in isolates of Mycobacterium tuberculosis from Brazilian patients using a reverse-phase hybridization assay. J Microbiol Methods, 2003, 53: 335-342.
23.	Traore H, Fissette K, Bastian I, et al. Detection of Rifampicin resistance in Mycobacterium tuberculosis isolates from diverse countries by a commercial line probe assay as an initial indicator of multidrug resistance. Int J Tuberc Lung Dis, 2000, 4: 481-484.
24.	De Beenhouwer H, Lhiang Z, Jannes G, et al. Rapid detection of Rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay. Tuber Lung Dis, 1995, 76: 425-430.
25.	Lemus D, Martin A, Montoro E, et al. Rapid alternative methods for detection of Rifampicin resistance in Mycobacterium tuberculosis. J Antimicrob Chemother, 2004, 54: 130-133.
26.	Watterson S, Wilson S, Yates M, et al. Comparison of three molecular assays for rapid detection of rifampin resistance in Mycobacterium tuberculosis. J Clin Microbiol, 1998, 36: 1969-1973.
27.	Srivastava K, Das R, Jakhmola P, et al. Correlation of mutations detected by INNO-LiPA with levels of rifampicin resistance in Mycobacterium tuberculosis. Indian J Med Res, 2004, 120(2): 100-105.
28.	Traore H, van Deun A, Shamputa IC, et al. Direct detection of Mycobacterium tuberculosis complex DNA and rifampin resistance in clinical specimens from tuberculosis patients by line probe assay. J Clin Microbiol, 2006, 44(12): 4384-4388.
29.	Marttila HJ, Soini H, Vyshnevskaya E, et al. Line probe assay in the rapid detection of rifampin-resistant Mycobacterium tuberculosis directly from clinical specimens. Scand J Infect Dis, 1999, 31(3): 269-273.
30.	Kisa O, Albay A, Bedir O, et al. Evaluation of FASTPlaquetb-RIF for determination of rifampicin resistance in Mycobacterium tuberculosis complex isolates. Int J Tuberc Lung Dis, 2003, 7(3): 284-288.
31.	Albert H, Trollip AP, Mole RJ, et al. Rapid indication of multidrug-resistant tuberculosis from liquid cultures using FASTPlaqueTB-RIF, a manual phage-based test. Int J Tuberc Lung Dis, 2002, 6(6): 523-528.
32.	Butt T, Ahmad RN, Afzal RK, et al. Rapid detection of rifampicin susceptibility of Mycobacterium tuberculosis in sputum specimens by mycobacteriophage assay. J Pak Med Assoc, 2004, 54(7): 379-382.
33.	Krishnamurthy A, Rodrigues C, Mehta AP. Rapid detection of rifampicin resistance in M. tuberculosis by phage assay. Ind J Med Microbiol, 2002, 20(4): 211-214.
34.	Albert H, Heydenrych A, Mole R, et al. Evaluation of FASTPlaque TB-RIF, a rapid, manual test for the determination of rifampicin resistance from Mycobacterium tuberculosis cultures. Int J Tuberc Lung Dis, 2001, 5(10): 906-911.
35.	Oguz VA, Guneri S, Erdenizmenli M, et al. Investigation of rifampin resistance in Mycobacterium tuberculosis by two different methods. Toraks Dergisi, 2002, 3(2): 173-177.
36.	Albert H, Trollip A, Seaman T, et al. Simple, phage-based (FASTPplaque) technology to determine rifampicin resistance of Mycobacterium tuberculosis directly from sputum. Int J Tuberc Lung Dis, 2004, 8(9): 1114-1119.
37.	Gali N, Dominguez J, Blanco S, et al. Utility of an in-house mycobacteriophage-based assay for rapid detection of rifampin resistance in Mycobacterium tuberculosis clinical isolates. J Clin Microbiol, 2003, 41(6): 2647-2649.
38.	Hamidou Traore, Ogwang S, Mallard K, et al. Low-cost rapid detection of rifampicin resistant tuberculosis using bacteriophage in Kampala, Uganda. Ann Clin Microbiol Antimicrob, 2007, 6: 1.
39.	Simboli N, Takiff H, McNerney R, et al. In-house phage amplification assay is a sound alternative for detecting rifampin-resistant Mycobacterium tuberculosis in low-resource settings. Antimicrob Agents Chemother, 2005, 49(1): 425-427.
40.	McNerney R. TB: the return of the phage. A review of fifty years of mycobacteriophage research. Int J Tuberc Lung Dis, 1999, 3(3): 179-184.
41.	Mani C, Selvakumar N, Kumar V, et al. Comparison of DNA sequencing, PCR-SSCP and PhaB assays with indirect sensitivity testing for detection of rifampicin resistance in Mycobacterium tuberculosis. Int J Tuberc Lung Dis, 2003, 7(7): 652-659.
42.	Eltringham IJ, Drobniewski FA, Mangan JA, et al. Evaluation of reverse transcription-PCR and a bacteriophage- based assay far rapid phenotypic detection of rifampin resistance in clinical isolates of Mycobacterium tuberculosis. J Clin Microbiol, 1999, 37(11): 3524-3527.
43.	Wilson SM, Al-Suwaidi Z, McNerney R, et al. Evaluation of a new rapid bacteriophagebased method for the drug susceptibility testing of Mycobacterium tuberculosis. Nat Med, 1997, 3(4): 465-468.
44.	Banaiee N, Bobadilla-del-Valle M, Riska PF, et al. Rapid identification and susceptibility testing of Mycobacterium tuberculosis from MGIT cultures with luciferase reporter mycobacteriophages. J Med Microbiol, 2003, 52(Pt 7): 557-561.
45.	Banaiee N, Bodadilla-del-Valle M, Bardarov S, et al. Luciferase reporter mycobacteriophages for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis in Mexico. J Clin Microbiol, 2001, 39(11): 3883-3888.
46.	Banaiee N, January V, Barthus C, et al. Semi-automation and evaluation of luciferase reporter mycobacteriophages for susceptibility testing of Mycobacterium tuberculosis cultures in South Africa. American Society for Microbiology 104th general meeting 2004, New Orleans, LA 2004 [Abstract U-018].
47.	Hazbon MH. Recent advances in molecular methods for early diagnosis of tuberculosis and drug-resistant tuberculosis. Biomedical, 2004, 24(Suppl 1): 149-162.
48.	Hazbon MH, Guarin N, Ferro BE, et al. Photographic and luminometric detection of luciferase reporter phages for drug susceptibility testing of clinical Mycobacterium tuberculosis isolates. J Clin Microbiol, 2003, 41(10): 4865-4869.
49.	Riska PF, Su Y, Bardarov S, et al. Rapid film-based determination of antibiotic susceptibilities of Mycobacterium tuberculosis strains by using a luciferase reporter phage and the Bronx box. J Clin Microbiol, 1999, 37(4): 1144-1149.
50.	Lu B, Fu Z, Xu S. Rapid rifampicin susceptibility test by using recombinant mycobacteriophages. Zhonghua Jie He He Hu Xi Za Zhi, 2000, 23(8): 480-484.
51.	Kalantri SP. Bacteriophage-based tests for the detection of Mycobacterium tuberculosis in clinical specimens: a systematic review and meta-analysis. Master’s in Public Health (MPH) Thesis, University of California, Berkeley, 2005.
52.	Símboli N, Takiff H, McNerney R, et al. In-house phage amplification assay is a sound alternative for detecting rifampin-resistant Mycobacterium tuberculosis in low-resource settings. Antimicrob Agents Chemother, 2005, 49(1): 425-427.

1. The WHO/IUATLD Global Project on Anti-Tuberculosis Drug Resistance Surveillance: Anti-Tuberculosis drug resistance in the world, report number 2 Volume. Geneva; 2004.
2. 王巍. 耐多药结核病的现状及诊断、处理对策. 军医进修学院学报, 2006, 27(6): 471-471.
3. 全国结核病流行病学抽样调查技术指导组. 第三次全国全国结核病流行病学抽样调查报告. 中华结核与呼吸杂志, 1992, 15(2): 69271.
4. Fisher M. Diagnosis of MDR-TB: a developing world problem on a developed world budget. Expert Rev Mol Diagn, 2002, 2(2): 151-159.
5. Parsons LM, Somoskovi A, Urbanczik R, et al. Laboratory diagnostic aspects of drug resistant tuberculosis. Front Biosci, 2004, 9: 2086-2105.
6. Hirano K, Abe C, Takahashi M. Mutations in the rpo B gene of rifampin-resistant Mycobacterium tuberculosis strains isolated mostly in Asian countries and their rapid detection by line probe assay. J Clin Microbiol, 1999, 37: 2663-2666.
7. Heifets LB, Cangelosi GA. Drug susceptibility testing of Mycobacterium tuberculosis: a neglected problem at the turn of the century. Int J Tuberc Lung Dis, 1999, 3(7): 564–581.
8. Cavusoglu C, Hilmioglu S, Guneri S, et al. Characterization of rpo B mutations in Rifampin-resistant clinical isolates of Mycobacterium tuberculosis from Turkey by DNA sequencing and line probe assay. J Clin Microbiol, 2002, 40: 4435-4438.
9. Hazbon MH. Recent advances in molecular methods for early diagnosis of tuberculosis and drug-resistant tuberculosis. Biomedical, 2004, 24(Suppl 1): 149-162.
10. Somoskovi A, Parsons LM, Salfinger M. The molecular basis of resistance to isoniazid, rifampin, and pyrazinamide in Mycobacterium tuberculosis. Respir Res, 2001, 2(3): 164-168.
11. Ahmad S, Mokaddas E, Fares E. Characterization of rpo B mutationsin rifampin-resistant clinical Mycobacterium tuberculosis isolates from Kuwait and Dubai. Diagn Microbiol Infect Dis, 2002, 44: 245-252.
12. Rossau R, Traore H, De Beenhouwer H, et al. Evaluation of the INNO-LiPA Rif. TB assay, areverse hybridization assay for the simultaneous detection of Mycobacterium tuberculosis complex and its resistance to rifampin. Antimicrob Agents Chemother, 1997, 41: 2093-2098.
13. Watterson S, Wilson S, Yates M, et al. Comparison of three molecular assays for rapid detection of rifampin resistanc in Mycobacterium tuberculosis. J Clin Microbiol, 1998, 36: 1969-1973.
14. Whiting P, Rutjes AW, Reitsma JB, et al. The development of QUADAS: a tool for the quality assessment of studies of diagnostic accuracy included in systematic reviews. BMC Med Res Methodol, 2003, 3: 25.
15. Gamboa F, Cardona PJ, Manterola JM, et al. Evaluation of a commercial probe assay fordetection of rifampin resistance in Mycobacterium tuberculosis directly from respiratory and nonrespiratory clinical samples. Eur J Clin Microbiol Infect Dis, 1998, 17: 189-192.
16. Johansen I, Lundgren B, Sosnovskaja A, et al. Direct detection of multidrug-resistant Mycobacterium tuberculosis in clinical specimens in low- and high-incidence countries by line probe assay. J Clin Microbiol, 2003, 41: 4454-4456.
17. Jureen P, Werngren J, Hoffner S. Evaluation of the line probe assay (LiPA) for rapid detection of Rifampicin resistance in Mycobac- terium tuberculosis. Tuberculosis, 2004, 84: 311-316.
18. Sintchenko V, Chew W, Jelfs P, et al. Mutations in rpo B gene and rifabutin susceptibility of multidrug-resistant Mycobacterium tuberculosis strains isolated in Australia. Pathology, 1999, 31: 257-260.
19. Tracevska T, Jansone I, Broka L, et al. Mutations in the rpo B and katG genes leading to drug resistance in Mycobacterium tuberculosis in Latvia. J Clin Microbiol, 2002, 40: 3789-3792.
20. Johansen I, Lundgren B, Sosnovskaja A, et al. Direct detection of multidrug-resistant Mycobacterium tuberculosis in clinical specimens in low- and high-incidence countries by line probe assay. J Clin Microbiol, 2003, 41: 4454-4456.
21. Viveiros M, Leandro C, Rodrigues L, et al. Direct application of the INNO-LiPA Rif.TB line-probe assay for rapid identification of Mycobacterium tuberculosis complex strains and detection of rifampin resistance in 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis. J Clin Microbiol, 2005, 43(9): 4880-4884.
22. De Oliveira M, Rocha A, Oelemann M, et al. Rapid detection of resistance against Rifampicin in isolates of Mycobacterium tuberculosis from Brazilian patients using a reverse-phase hybridization assay. J Microbiol Methods, 2003, 53: 335-342.
23. Traore H, Fissette K, Bastian I, et al. Detection of Rifampicin resistance in Mycobacterium tuberculosis isolates from diverse countries by a commercial line probe assay as an initial indicator of multidrug resistance. Int J Tuberc Lung Dis, 2000, 4: 481-484.
24. De Beenhouwer H, Lhiang Z, Jannes G, et al. Rapid detection of Rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay. Tuber Lung Dis, 1995, 76: 425-430.
25. Lemus D, Martin A, Montoro E, et al. Rapid alternative methods for detection of Rifampicin resistance in Mycobacterium tuberculosis. J Antimicrob Chemother, 2004, 54: 130-133.
26. Watterson S, Wilson S, Yates M, et al. Comparison of three molecular assays for rapid detection of rifampin resistance in Mycobacterium tuberculosis. J Clin Microbiol, 1998, 36: 1969-1973.
27. Srivastava K, Das R, Jakhmola P, et al. Correlation of mutations detected by INNO-LiPA with levels of rifampicin resistance in Mycobacterium tuberculosis. Indian J Med Res, 2004, 120(2): 100-105.
28. Traore H, van Deun A, Shamputa IC, et al. Direct detection of Mycobacterium tuberculosis complex DNA and rifampin resistance in clinical specimens from tuberculosis patients by line probe assay. J Clin Microbiol, 2006, 44(12): 4384-4388.
29. Marttila HJ, Soini H, Vyshnevskaya E, et al. Line probe assay in the rapid detection of rifampin-resistant Mycobacterium tuberculosis directly from clinical specimens. Scand J Infect Dis, 1999, 31(3): 269-273.
30. Kisa O, Albay A, Bedir O, et al. Evaluation of FASTPlaquetb-RIF for determination of rifampicin resistance in Mycobacterium tuberculosis complex isolates. Int J Tuberc Lung Dis, 2003, 7(3): 284-288.
31. Albert H, Trollip AP, Mole RJ, et al. Rapid indication of multidrug-resistant tuberculosis from liquid cultures using FASTPlaqueTB-RIF, a manual phage-based test. Int J Tuberc Lung Dis, 2002, 6(6): 523-528.
32. Butt T, Ahmad RN, Afzal RK, et al. Rapid detection of rifampicin susceptibility of Mycobacterium tuberculosis in sputum specimens by mycobacteriophage assay. J Pak Med Assoc, 2004, 54(7): 379-382.
33. Krishnamurthy A, Rodrigues C, Mehta AP. Rapid detection of rifampicin resistance in M. tuberculosis by phage assay. Ind J Med Microbiol, 2002, 20(4): 211-214.
34. Albert H, Heydenrych A, Mole R, et al. Evaluation of FASTPlaque TB-RIF, a rapid, manual test for the determination of rifampicin resistance from Mycobacterium tuberculosis cultures. Int J Tuberc Lung Dis, 2001, 5(10): 906-911.
35. Oguz VA, Guneri S, Erdenizmenli M, et al. Investigation of rifampin resistance in Mycobacterium tuberculosis by two different methods. Toraks Dergisi, 2002, 3(2): 173-177.
36. Albert H, Trollip A, Seaman T, et al. Simple, phage-based (FASTPplaque) technology to determine rifampicin resistance of Mycobacterium tuberculosis directly from sputum. Int J Tuberc Lung Dis, 2004, 8(9): 1114-1119.
37. Gali N, Dominguez J, Blanco S, et al. Utility of an in-house mycobacteriophage-based assay for rapid detection of rifampin resistance in Mycobacterium tuberculosis clinical isolates. J Clin Microbiol, 2003, 41(6): 2647-2649.
38. Hamidou Traore, Ogwang S, Mallard K, et al. Low-cost rapid detection of rifampicin resistant tuberculosis using bacteriophage in Kampala, Uganda. Ann Clin Microbiol Antimicrob, 2007, 6: 1.
39. Simboli N, Takiff H, McNerney R, et al. In-house phage amplification assay is a sound alternative for detecting rifampin-resistant Mycobacterium tuberculosis in low-resource settings. Antimicrob Agents Chemother, 2005, 49(1): 425-427.
40. McNerney R. TB: the return of the phage. A review of fifty years of mycobacteriophage research. Int J Tuberc Lung Dis, 1999, 3(3): 179-184.
41. Mani C, Selvakumar N, Kumar V, et al. Comparison of DNA sequencing, PCR-SSCP and PhaB assays with indirect sensitivity testing for detection of rifampicin resistance in Mycobacterium tuberculosis. Int J Tuberc Lung Dis, 2003, 7(7): 652-659.
42. Eltringham IJ, Drobniewski FA, Mangan JA, et al. Evaluation of reverse transcription-PCR and a bacteriophage- based assay far rapid phenotypic detection of rifampin resistance in clinical isolates of Mycobacterium tuberculosis. J Clin Microbiol, 1999, 37(11): 3524-3527.
43. Wilson SM, Al-Suwaidi Z, McNerney R, et al. Evaluation of a new rapid bacteriophagebased method for the drug susceptibility testing of Mycobacterium tuberculosis. Nat Med, 1997, 3(4): 465-468.
44. Banaiee N, Bobadilla-del-Valle M, Riska PF, et al. Rapid identification and susceptibility testing of Mycobacterium tuberculosis from MGIT cultures with luciferase reporter mycobacteriophages. J Med Microbiol, 2003, 52(Pt 7): 557-561.
45. Banaiee N, Bodadilla-del-Valle M, Bardarov S, et al. Luciferase reporter mycobacteriophages for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis in Mexico. J Clin Microbiol, 2001, 39(11): 3883-3888.
46. Banaiee N, January V, Barthus C, et al. Semi-automation and evaluation of luciferase reporter mycobacteriophages for susceptibility testing of Mycobacterium tuberculosis cultures in South Africa. American Society for Microbiology 104th general meeting 2004, New Orleans, LA 2004 [Abstract U-018].
47. Hazbon MH. Recent advances in molecular methods for early diagnosis of tuberculosis and drug-resistant tuberculosis. Biomedical, 2004, 24(Suppl 1): 149-162.
48. Hazbon MH, Guarin N, Ferro BE, et al. Photographic and luminometric detection of luciferase reporter phages for drug susceptibility testing of clinical Mycobacterium tuberculosis isolates. J Clin Microbiol, 2003, 41(10): 4865-4869.
49. Riska PF, Su Y, Bardarov S, et al. Rapid film-based determination of antibiotic susceptibilities of Mycobacterium tuberculosis strains by using a luciferase reporter phage and the Bronx box. J Clin Microbiol, 1999, 37(4): 1144-1149.
50. Lu B, Fu Z, Xu S. Rapid rifampicin susceptibility test by using recombinant mycobacteriophages. Zhonghua Jie He He Hu Xi Za Zhi, 2000, 23(8): 480-484.
51. Kalantri SP. Bacteriophage-based tests for the detection of Mycobacterium tuberculosis in clinical specimens: a systematic review and meta-analysis. Master’s in Public Health (MPH) Thesis, University of California, Berkeley, 2005.
52. Símboli N, Takiff H, McNerney R, et al. In-house phage amplification assay is a sound alternative for detecting rifampin-resistant Mycobacterium tuberculosis in low-resource settings. Antimicrob Agents Chemother, 2005, 49(1): 425-427.

Previous Article
Analyses of Length of Hospital Stay and Expenses Associated with Hysterectomy
Next Article
Local versus Systemic Application of Opioids for Labor Analgesia: A Systematic Review

Chinese Journal of Evidence-Based Medicine

Diagnostic Value of LiPA and Phage-Based Assays for Rifampicin Resistance in Mycobacterium Tuberculosis: A Systematic Review

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content