Objective To study oxygen carrying capacity of red blood cell in children with congenital left-to-right shunt (CLRS) and discuss its clinical significance.
Methods A total of 62 children with CLRS were selected as a trial group and 40 healthy children who had accepted clinical physical examination as a control group. ELISA test was applied to determine 2,3 -DPG content of red blood cells. At the same time, pH, PaO2, PaCO2, Hb, P50O2 and relevant outcomes were tested for correlation analysis.
Results Red blood cell 2,3-DPG (2.76±0.98 μmol/mL) was higher than that of the control group. PaO2, pH, Hb were lower than those of the control group with significant differences. Among the types of CLRS, 2,3 -DPG of ventricular septal defect was the highest and that of patent ductus arteriosus ranked the second, the two of which had a significant difference (P=0.007). As for red blood cell of children with congenital heart diseases, there was linear correlation between 2,3- DPG and PaO2 as [2,3-DPG=12.007 8– (0.154 7×PaO2)], as well as between P50O2 and 2,3 -DPG as [P50O2=26.303 6+ (1.799 2×2,3-DPG)].
Conclusion Children with congenital left-to-right shunt tend to have low oxygen. Therefore, it is important to detect 2,3-DPG of red blood cell, blood gas analysis, and hemoglobin level change, in order to well understand the mechanism of congenital heart disease as well as to guide clinical practice.
Citation：
YANG Yinan,DONG Xiangyu,YU Mu,NI Qian,SHEN Yang,LE Gaozhong. Study on Oxygen Carrying Capacity of Red Blood Cell in Children with Congenital Left-to-Right Shunt. Chinese Journal of Evidence-Based Medicine, 2012, 12(9): 1052-1055. doi: 10.7507/1672-2531.20120164
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Copyright © the editorial department of Chinese Journal of Evidence-Based Medicine of West China Medical Publisher. All rights reserved
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- 1. 仇慧仙, 张园海, 陈其, 等. 先天性心脏病合并其他畸形病例分析. 中国当代儿科杂志, 2011, 13(8): 686-687.
- 2. Rosenthal A, William CM, Esther BE, et al. The role of red blood cell organic phosphates in adaption to congenital heart disease. American Academy of Pediatrics, 1971, 47(3): 537-547.
- 3. Eaton JW, Brewer GJ, Schultz JS. Variation in 2, 3-diphosphoglycerateand ATP levels in human erythrocytes and effects on oxygen transport. Exp Med Biol, 1970, 6(6): 21-38.
- 4. Dubey ML, Hegde R, Ganguly NK, et al. Decreased level of2, 3-diphosphoglycerate and alteration of structural integrity in erythrocytes infected with Plasmodium falciparum in vitro. Molecular and Cellular Biochemistry, 2003, 246(18): 137-141.
- 5. 何晖, 刘宝林, 华泽钊, 等. 甘油保护剂对冷冻干燥保存红细胞作用的实验研究. 上海理工大学学报, 2006, 28(5): 413-417.
- 6. 吴艳丽, 陆典瑞, 胡继红, 等. 影响冷冻红细胞质量的若干因素研究. 中华煤炭工业医学杂志, 2007, 10(12): 1412-1413.
- 7. 王凤明, 程彩虹, 王晓东. 5927例0-2岁儿童先天性心脏病患病调查分析. 疾病监测, 2008, 23(8): 508-509.
- 8. 金英华, 麻静. 先天性心脏病缺陷监测及干预对策. 中国妇幼保健, 2007, 22(29): 1420-1421.
- 9. Pradat P, Francannet C, Harris JA, et al. The epidemiology of cardiovascular defects, part I: a study based on data from three large registries of congenital malformations. Pediatr Cardio, 2003, 24(8): 195.
- 10. 陈秋红, 路霖, 祁国荣, 等. 高原地区先天性心脏病并发肺动脉高压的调查分析. 中华医学杂志, 2011, 91(44): 3120-3122.
- 11. 刘红, 刘淑文. 甘肃省5岁以下儿童先天异常死亡5年检测分析. 卫生职业教育, 2006, 24(17): 97-98.
- 12. Kavdia M, Pittman RN, Popel AS. Theoretical analysis of effects of blood substitute affinity and cooperativity on organ oxygen transport. J Appl Physiol, 2002, 93(3): 2122-2128.
