Diagnostic Value of ELISA Using Antigen B in Cystic Echinococcosis: A Meta-Analysis_Chinese Journal of Evidence-Based Medicine

Authors：

LI Yanhong , MENG Cunren ,  ZHANG Zhaoxiao

Department of Laboratory Medicine, The First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China;

Corresponding author：

ZHANG Zhaoxiao, Email: Xia0513@yahoo.com.cn

Keywords：

Cystic echinococcosis; Antigen B; ELISA; Meta-analysis; Diagnostic test

DOI：

10.7507/1672-2531.20130057

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective 　To evaluate the diagnostic value of ELISA using AgB in cystic echinococcosis (CE).
Methods 　Such databases as PubMed, EMbase, The Cochrane Library, EBSCO, CBM, CNKI, WanFang Data and MedaLink were retrieved on computer, and the relevant journals were also manually searched to collect the trials on ELISA using AgB in diagnosis of CE. The retrieval time was from inception to July 5th, 2012. Two reviewers independently screened the literature, extracted the data and assessed the quality according to QUADAS. Then the meta-analysis was conducted by using Meta-Disc 1.4 software.
Results 　A total of 8 studies were included, and there were 562 CE patients diagnosed by gold standard, 434 suspected cases and 303 healthy people. There were no threshold effects among those 8 studies (the spearman’s correlation coefficient of log sensitivity to log 1-specificity was 0.527, P=0.400 1). The meta-analysis of DerSimonia-Laird showed that, the pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio and diagnostic odds ratio was 0.76 (95%CI 0.73 to 0.79), 0.84 (95%CI 0.82 to 0.86), 5.20 (95%CI 3.59 to 7.55), 0.26 (95%CI 0.18 to 0.35), 23.93 (95%CI 12.35 to 46.39), respectively. And the AUC of SROC was 0.889 7 (Q=0.820 4).
Conclusion 　ELISA using natural AgB and rAgB has greater diagnostic value in detecting CE.

Citation： LI Yanhong,MENG Cunren,ZHANG Zhaoxiao. Diagnostic Value of ELISA Using Antigen B in Cystic Echinococcosis: A Meta-Analysis. Chinese Journal of Evidence-Based Medicine, 2013, 13(3): 332-338. doi: 10.7507/1672-2531.20130057 Copy

1. 刘洪波, 谢妍, 王宁. 包虫病. 中国实用乡村医生杂志, 2006, 13(11): 10-11.

2. Musiani P, Piantelli M, Lauriola L, et al. Echinococcus granulosus: specific quantification of the two most immunoreactive antigens in hydatid fluids. J Clin Pathol, 1978, 31(5): 475-478.

3. HaAg KL, Zanotto PM, Alves-Junior L, et al. Searching for antigen B genes and their adaptive sites in distinct strains and species of the helminth Echinococcus. Infect Genet Evol, 2006, 6(4): 251-261.

4. HaAg KL, Gott stein B, Muller N, et al. Redundancy and recombination in the Echinococcus AgB multigene family: is there any similarity with protozoan contingency genes? Parasitol, 2006, 133(4): 411-419.

5. Mamuti W, Sako Y, Bart JM, et al. Molecular characterization of anovelgeneencodingan8kDasubunit of antigen B from Echinococcus granulosus genotypes 1 and 6. Parasitol Int, 2007, 56(2): 313-316.

6. Parija SC. A review of some simple immunoassays in the serodiAgnosis of cystic hydatid disease. Acta Trop, 1998, 70(1): 17-24.

7. Paul M, Stefaniak J. Comparison of the dot immunobinding assay and two enzyme-linked immunosorbent assay kits for the diAgnosis of liver cystic echinococcosis. Hepatol Res, 2001, 21(1): 14-26.

8. Whiting P, Rutjes AW, Reitsma JB, 等. QUADAS的制定: 用于系统评价中评价诊断性研究质量的工具. 中国循证医学杂志, 2007, 7(4): 296-306.

9. Whiting P, Weswood ME, Rutjes AW, 等. QUADAS评价: 一种用于诊断性研究的质量评价工具(修订版). 中国循证医学杂志, 2007, 7(7): 531-536.

10. Sbihi Y, Rmiqui A, Rodriguez-Cabezas MN, et al. Comparative sensitivity of six serological tests and diAgnostic value of ELISA using purified antigen in hydatidosis. J Clin Lab Anal, 2001, 15(1): 14-18.

11. Sadjjadi SM, Abidi H, Sarkari B, et al. Evaluation of enzyme linked immunosorbent assay, utilizing native antigen B for serodiAgnosis of human hydatidosis. Iran J Immunol, 2007, 4(3): 167-172.

12. González-Sapienza G, Lorenzo C, Nieto A. Improved immunodiAgnosis of cystic hydatid disease by using a synthetic peptide with higher diAgnostic value than that of its parent protein, Echinococcus granulosus antigen B. J Clin Microbiol, 2000, 38(11): 3979-3983.

13. Kalantari E, Bandehpour M, Pazoki R, et al. Application of recombinant Echinococcus granulosus antigen B to ELISA kits for diAgnosing hydatidosis. Parasitol Res, 2010, 106(4): 847-851.

14. 江莉, 冯正, 许学年. 细粒棘球蚴AgB亚单位基因的联合表达和血清学评价. 热带医学杂志, 2009, 9(1): 25-41.

15. Jiang L, Zhang YG, Liu MX, et al. Analysis on the reactivity of five subunits of antigen B family in serodiAgnosis of echinococcosis. Exp Parasitol, 2012, 131(1): 85-91.

16. 高春花, 崔刚, 王俊云, 等. 我国细粒棘球绦虫新疆分离柱AgB8/3亚基克隆表达及其检测囊型包虫病价值分析. 中国人兽共患病学报, 2011, 27(11): 966-969.

17. 马秀敏, 乌拉木·马木提, 马海梅, 等. 细粒棘球蚴重组AgB8-kDa 亚单位1对囊型包虫病的血清学诊断价值. 中国人兽共患病学报, 2009, 25(8), 741-744.

18. Hadgu A. The discrepancy in discrepant analysis. Lancet, 1996, 348(9027): 592-593.

19. Kato I, Santamaria M, De Ruiz PA, et al. Inter-observer variation in cytological and histological diAgnoses of cervical neoplasia and its epidemiologic implication. J Clin Epidemiol, 1995, 48(9): 1167-1174.

20. Tackmann R, Schuetz G, Hamm B, et al. Quality of the reporting of diAgnostic accuracy studies: STARD (Standards for the Reporting of DiAgnostic accuracy studies). Rofo, 2010, 182(8): 655-659.

21. 秦亮, 移康, 马彬, 等. 抗角蛋白抗体对类风湿性关节炎诊断价值的系统评价. 中国循证医学杂志, 2011, 11(3): 265 -272.

1. 刘洪波, 谢妍, 王宁. 包虫病. 中国实用乡村医生杂志, 2006, 13(11): 10-11.

2. Musiani P, Piantelli M, Lauriola L, et al. Echinococcus granulosus: specific quantification of the two most immunoreactive antigens in hydatid fluids. J Clin Pathol, 1978, 31(5): 475-478.

3. HaAg KL, Zanotto PM, Alves-Junior L, et al. Searching for antigen B genes and their adaptive sites in distinct strains and species of the helminth Echinococcus. Infect Genet Evol, 2006, 6(4): 251-261.

4. HaAg KL, Gott stein B, Muller N, et al. Redundancy and recombination in the Echinococcus AgB multigene family: is there any similarity with protozoan contingency genes? Parasitol, 2006, 133(4): 411-419.

5. Mamuti W, Sako Y, Bart JM, et al. Molecular characterization of anovelgeneencodingan8kDasubunit of antigen B from Echinococcus granulosus genotypes 1 and 6. Parasitol Int, 2007, 56(2): 313-316.

6. Parija SC. A review of some simple immunoassays in the serodiAgnosis of cystic hydatid disease. Acta Trop, 1998, 70(1): 17-24.

7. Paul M, Stefaniak J. Comparison of the dot immunobinding assay and two enzyme-linked immunosorbent assay kits for the diAgnosis of liver cystic echinococcosis. Hepatol Res, 2001, 21(1): 14-26.

8. Whiting P, Rutjes AW, Reitsma JB, 等. QUADAS的制定: 用于系统评价中评价诊断性研究质量的工具. 中国循证医学杂志, 2007, 7(4): 296-306.

9. Whiting P, Weswood ME, Rutjes AW, 等. QUADAS评价: 一种用于诊断性研究的质量评价工具(修订版). 中国循证医学杂志, 2007, 7(7): 531-536.

10. Sbihi Y, Rmiqui A, Rodriguez-Cabezas MN, et al. Comparative sensitivity of six serological tests and diAgnostic value of ELISA using purified antigen in hydatidosis. J Clin Lab Anal, 2001, 15(1): 14-18.

11. Sadjjadi SM, Abidi H, Sarkari B, et al. Evaluation of enzyme linked immunosorbent assay, utilizing native antigen B for serodiAgnosis of human hydatidosis. Iran J Immunol, 2007, 4(3): 167-172.

12. González-Sapienza G, Lorenzo C, Nieto A. Improved immunodiAgnosis of cystic hydatid disease by using a synthetic peptide with higher diAgnostic value than that of its parent protein, Echinococcus granulosus antigen B. J Clin Microbiol, 2000, 38(11): 3979-3983.

13. Kalantari E, Bandehpour M, Pazoki R, et al. Application of recombinant Echinococcus granulosus antigen B to ELISA kits for diAgnosing hydatidosis. Parasitol Res, 2010, 106(4): 847-851.

14. 江莉, 冯正, 许学年. 细粒棘球蚴AgB亚单位基因的联合表达和血清学评价. 热带医学杂志, 2009, 9(1): 25-41.

15. Jiang L, Zhang YG, Liu MX, et al. Analysis on the reactivity of five subunits of antigen B family in serodiAgnosis of echinococcosis. Exp Parasitol, 2012, 131(1): 85-91.

16. 高春花, 崔刚, 王俊云, 等. 我国细粒棘球绦虫新疆分离柱AgB8/3亚基克隆表达及其检测囊型包虫病价值分析. 中国人兽共患病学报, 2011, 27(11): 966-969.

17. 马秀敏, 乌拉木·马木提, 马海梅, 等. 细粒棘球蚴重组AgB8-kDa 亚单位1对囊型包虫病的血清学诊断价值. 中国人兽共患病学报, 2009, 25(8), 741-744.

18. Hadgu A. The discrepancy in discrepant analysis. Lancet, 1996, 348(9027): 592-593.

19. Kato I, Santamaria M, De Ruiz PA, et al. Inter-observer variation in cytological and histological diAgnoses of cervical neoplasia and its epidemiologic implication. J Clin Epidemiol, 1995, 48(9): 1167-1174.

20. Tackmann R, Schuetz G, Hamm B, et al. Quality of the reporting of diAgnostic accuracy studies: STARD (Standards for the Reporting of DiAgnostic accuracy studies). Rofo, 2010, 182(8): 655-659.

21. 秦亮, 移康, 马彬, 等. 抗角蛋白抗体对类风湿性关节炎诊断价值的系统评价. 中国循证医学杂志, 2011, 11(3): 265 -272.

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Diagnostic Value of ELISA Using Antigen B in Cystic Echinococcosis: A Meta-Analysis

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1.	刘洪波, 谢妍, 王宁. 包虫病. 中国实用乡村医生杂志, 2006, 13(11): 10-11.
2.	Musiani P, Piantelli M, Lauriola L, et al. Echinococcus granulosus: specific quantification of the two most immunoreactive antigens in hydatid fluids. J Clin Pathol, 1978, 31(5): 475-478.
3.	HaAg KL, Zanotto PM, Alves-Junior L, et al. Searching for antigen B genes and their adaptive sites in distinct strains and species of the helminth Echinococcus. Infect Genet Evol, 2006, 6(4): 251-261.
4.	HaAg KL, Gott stein B, Muller N, et al. Redundancy and recombination in the Echinococcus AgB multigene family: is there any similarity with protozoan contingency genes? Parasitol, 2006, 133(4): 411-419.
5.	Mamuti W, Sako Y, Bart JM, et al. Molecular characterization of anovelgeneencodingan8kDasubunit of antigen B from Echinococcus granulosus genotypes 1 and 6. Parasitol Int, 2007, 56(2): 313-316.
6.	Parija SC. A review of some simple immunoassays in the serodiAgnosis of cystic hydatid disease. Acta Trop, 1998, 70(1): 17-24.
7.	Paul M, Stefaniak J. Comparison of the dot immunobinding assay and two enzyme-linked immunosorbent assay kits for the diAgnosis of liver cystic echinococcosis. Hepatol Res, 2001, 21(1): 14-26.
8.	Whiting P, Rutjes AW, Reitsma JB, 等. QUADAS的制定: 用于系统评价中评价诊断性研究质量的工具. 中国循证医学杂志, 2007, 7(4): 296-306.
9.	Whiting P, Weswood ME, Rutjes AW, 等. QUADAS评价: 一种用于诊断性研究的质量评价工具(修订版). 中国循证医学杂志, 2007, 7(7): 531-536.
10.	Sbihi Y, Rmiqui A, Rodriguez-Cabezas MN, et al. Comparative sensitivity of six serological tests and diAgnostic value of ELISA using purified antigen in hydatidosis. J Clin Lab Anal, 2001, 15(1): 14-18.
11.	Sadjjadi SM, Abidi H, Sarkari B, et al. Evaluation of enzyme linked immunosorbent assay, utilizing native antigen B for serodiAgnosis of human hydatidosis. Iran J Immunol, 2007, 4(3): 167-172.
12.	González-Sapienza G, Lorenzo C, Nieto A. Improved immunodiAgnosis of cystic hydatid disease by using a synthetic peptide with higher diAgnostic value than that of its parent protein, Echinococcus granulosus antigen B. J Clin Microbiol, 2000, 38(11): 3979-3983.
13.	Kalantari E, Bandehpour M, Pazoki R, et al. Application of recombinant Echinococcus granulosus antigen B to ELISA kits for diAgnosing hydatidosis. Parasitol Res, 2010, 106(4): 847-851.
14.	江莉, 冯正, 许学年. 细粒棘球蚴AgB亚单位基因的联合表达和血清学评价. 热带医学杂志, 2009, 9(1): 25-41.
15.	Jiang L, Zhang YG, Liu MX, et al. Analysis on the reactivity of five subunits of antigen B family in serodiAgnosis of echinococcosis. Exp Parasitol, 2012, 131(1): 85-91.
16.	高春花, 崔刚, 王俊云, 等. 我国细粒棘球绦虫新疆分离柱AgB8/3亚基克隆表达及其检测囊型包虫病价值分析. 中国人兽共患病学报, 2011, 27(11): 966-969.
17.	马秀敏, 乌拉木·马木提, 马海梅, 等. 细粒棘球蚴重组AgB8-kDa 亚单位1对囊型包虫病的血清学诊断价值. 中国人兽共患病学报, 2009, 25(8), 741-744.
18.	Hadgu A. The discrepancy in discrepant analysis. Lancet, 1996, 348(9027): 592-593.
19.	Kato I, Santamaria M, De Ruiz PA, et al. Inter-observer variation in cytological and histological diAgnoses of cervical neoplasia and its epidemiologic implication. J Clin Epidemiol, 1995, 48(9): 1167-1174.
20.	Tackmann R, Schuetz G, Hamm B, et al. Quality of the reporting of diAgnostic accuracy studies: STARD (Standards for the Reporting of DiAgnostic accuracy studies). Rofo, 2010, 182(8): 655-659.
21.	秦亮, 移康, 马彬, 等. 抗角蛋白抗体对类风湿性关节炎诊断价值的系统评价. 中国循证医学杂志, 2011, 11(3): 265 -272.