Correlation between Beclin1 Protein Expression and Cervical Cancer Risk: A Meta-Analysis_Chinese Journal of Evidence-Based Medicine

Authors：

 WANGYu-juan ¹ , ZHANGJing ¹ , HUOJun-ling ¹ , LVJuan ¹ ,  WANGZan-hong ²

1. Shanxi Medical University, Taiyuan 030001, China;
2. Department of Gynecology, Shanxi Academy of Medical Sciences, Shanxi Da Yi Hospital, Taiyuan 030032, China;

Corresponding author：

WANGZan-hong, Email: wangzanhong@126.com

Keywords：

Beclin1; Cervical cancer; Systematic review; Meta-analysis; Case-control study

DOI：

10.7507/1672-2531.20140134

Video：

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Objective To systematically review the correlation between Beclin1 protein expression and cervical cancer as well as its different clinical pathologic features. Methods We electronically searched databases including The Cochrane Library (Issue 1, 2014), PubMed, EMbase, Ovid, CNKI, VIP, CBM and WanFang Data from inception to February 2014, to collect the correlation between Beclin1 protein expression and cervical cancer as well as its different clinical pathologic features. Two reviewers independently screened literature according to the inclusion and exclusion criteria, extracted data, and assessed the methodological quality of the included studies. Then meta-analysis was conducted using RevMan 5.2 software. Results A total of 5 case-control studies involving 637 patients were included, of which, 388 cases in the cervical cancer group, 130 cases in the cervical intraepithelial neoplasia (CIN) group, and 119 cases in the normal cervical tissue group. The results of meta-analysis showed that, a) as for Beclin1 expression, significant differences were found in cervical cancer vs. normal cervical tissues (OR=0.07, 95%CI 0.02 to 0.25, P < 0.000 1), cervical cancer vs. CIN (OR=0.37, 95%CI 0.23 to 0.59, P < 0.000 1), CIN vs. normal cervical tissues (OR=0.23, 95%CI 0.06 to 0.88, P=0.03), and cervical cancer tissues with vs. without lymph node metastasis (OR=0.29, 95%CI 0.17 to 0.49, P < 0.000 01). However, no significant difference was found in medium/low differentiation vs. well differentiation (OR=0.50, 95%CI 0.16 to 1.56, P=0.23), tumour diameter no less than vs. less than 4 cm (OR=0.72, 95%CI 0.44 to 1.18, P=0.20), myometrial invasion depth no less than vs. less than 1/2, and FIGO Ⅰ vs. Ⅱ (OR=0.70, 95%CI 0.44 to 1.10, P=0.12). Conclusion Beclin1 protein expression is notably associated to cervical cancer. Due to the limited quantity and quality of the included studies, the above conclusion still needs to be further verified by performing more high quality studies.

Citation： WANGYu-juan, ZHANGJing, HUOJun-ling, LVJuan, WANGZan-hong. Correlation between Beclin1 Protein Expression and Cervical Cancer Risk: A Meta-Analysis. Chinese Journal of Evidence-Based Medicine, 2014, 14(7): 798-805. doi: 10.7507/1672-2531.20140134 Copy

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12.	Wells G, Shea B, ÓConnell D, et al. Newcastle-Ottawa Quality Assessment Scale-Case Control Studies. 2012-06-15. Available at:http://www.ohri.ca/programs/clinical-epidemiology/oxford-asp.
13.	Stang A. Critical evaluation of the Newcastle-Ottawa scale for the assessment of the quality of nonrandomized studies in meta-analyses. Eur J epidemiol, 2010, 25(9):603-605.
14.	Shimizu S, Kanaseki T, Mizushima N. Role of Bcl-2 family proteins in a nonapoptotic programmed cell death dependent on autophagy genes. Nat Cell Biol, 2004, 6(12):1221-1228.
15.	Levine B, Yuan J. Autophagy in cell death:an innocent convict. Clin Invest, 2005, 11(5):2679-2688.
16.	Baehrecke EH. Autophagy:dual roles in life and death. Nat Rev Mol Cell Biol, 2005, 6(6):505-510.
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18.	Sun Y, Peng ZL. Autophagy, beclin1, and their relation with oncogenesis. Labmedicine, 2008, 39(5):287-290.
19.	Agarwal A, Balla J, Alam J. Induction of heme oxygenase in toxic renal injury:a protective role in cisplatin nephrotoxicity in the rat. Kidney Int, 1995, 48(4):1298-1307.
20.	程海燕, 张彦娜.自噬相关蛋白Beclin1、LC3在宫颈癌中的表达及临床意义.中山大学硕士论文, 2009.
21.	Jiang M, Wei Q, Pabla N. Effects of hydroxyl radical scavenging on cisplatin-induced p53 activation, tubular cell apoptosis an nephrotoxicity. Biochem Pharmacol, 2007, 73(9):1499-1510.
22.	Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J Virol, 1998, 72(11):8586-596.
23.	Aim VM, Liang XH, Murty VV, et al. Cloning and cnomic structure of beclin 1, a candidate tumor suppressor ene on chromosome 17q21. Genomics, 1999, 59(1):59-65.
24.	Oberstein A, Jeffrey PD, Shi Y. Crystal structure of the Bcl-XL-Beclin 1 peptide complex:Beclin 1 is a novel BH3-only protein. J Biol Chem, 2007, 282(17):13123-13132.
25.	钱学茜, 万小云.子宫内膜癌发生过程中自噬活性改变的初步探讨.现代妇产科进展, 2008, 17(7):517-519.

1. Parking DM, Bpray F, Ferlay J, et al. Global cancer statistics in 2002. CA Cancer J Clin, 2005, 55(2):74-108.
2. 石峰, 王明荣.细胞自噬及其与肿瘤关系的研究进展.中国细胞生物学学报, 2011, 33(12):1366-1373.
3. 孙阳, 刘佳华.妇科常见肿瘤中细胞自噬现象的研究进展.中华肿瘤防治杂志, 2011, 18(3):233-236.
4. Wang ZH, Xu L, Duan ZL, et al. Beclin 1-mediated macroautophagy involves regulation of caspase-9 expression in cervical cancer HeLa cells. Gynecol Oncol, 2007, 107(1):107-113.
5. Yue Z, Jin S, Yang C, et al. Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor. Proc Natl Acad Sci USA, 2003, 100(25):15077-15082.
6. Qu X, Yu J, Bhagat G, et al. Promotion of tumorigenesis by heterozygous disruption of the beclin1 autophagy gene. J Clin Invest, 2003, 112(12):1809-1820.
7. 王赞宏, 彭之兰.自噬基因Beclinl与宫颈癌相关性的实验研究.四川大学博士学位论文, 2007.
8. 吴瑞花, 郑署民. Beclin1、PTEN在子宫颈鳞癌中的表达、相关性及意义.山西医科大学硕士论文, 2011.
9. 潘小虹, 朱维培.自噬相关基因Beclin1和LC3II在早期宫颈癌组织中的表达及临床意义.兰州大学硕士论文, 2012.
10. Cheng HY, Zhang YN, et al. Expression of beclin 1, an autophagy-related protein, in human cervical carcinoma and its clinical significance. Eur J Gynaecol oncol, 2012, 33(1):15-20.
11. 李跃, 王渠源, 张颂婕, 等.自噬基因ARHI与Beclin1在宫颈癌中的表达及意义.中华临床医师杂志:电子版, 2013, 7(23):10648-10652.
12. Wells G, Shea B, ÓConnell D, et al. Newcastle-Ottawa Quality Assessment Scale-Case Control Studies. 2012-06-15. Available at:http://www.ohri.ca/programs/clinical-epidemiology/oxford-asp.
13. Stang A. Critical evaluation of the Newcastle-Ottawa scale for the assessment of the quality of nonrandomized studies in meta-analyses. Eur J epidemiol, 2010, 25(9):603-605.
14. Shimizu S, Kanaseki T, Mizushima N. Role of Bcl-2 family proteins in a nonapoptotic programmed cell death dependent on autophagy genes. Nat Cell Biol, 2004, 6(12):1221-1228.
15. Levine B, Yuan J. Autophagy in cell death:an innocent convict. Clin Invest, 2005, 11(5):2679-2688.
16. Baehrecke EH. Autophagy:dual roles in life and death. Nat Rev Mol Cell Biol, 2005, 6(6):505-510.
17. Boya P, Gonzalez-Polo RA, Casares N, et al. Inhibition of macroautophagy triggers apoptosis. Mol Cell Biol, 2005, 25(3):1025-1040.
18. Sun Y, Peng ZL. Autophagy, beclin1, and their relation with oncogenesis. Labmedicine, 2008, 39(5):287-290.
19. Agarwal A, Balla J, Alam J. Induction of heme oxygenase in toxic renal injury:a protective role in cisplatin nephrotoxicity in the rat. Kidney Int, 1995, 48(4):1298-1307.
20. 程海燕, 张彦娜.自噬相关蛋白Beclin1、LC3在宫颈癌中的表达及临床意义.中山大学硕士论文, 2009.
21. Jiang M, Wei Q, Pabla N. Effects of hydroxyl radical scavenging on cisplatin-induced p53 activation, tubular cell apoptosis an nephrotoxicity. Biochem Pharmacol, 2007, 73(9):1499-1510.
22. Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J Virol, 1998, 72(11):8586-596.
23. Aim VM, Liang XH, Murty VV, et al. Cloning and cnomic structure of beclin 1, a candidate tumor suppressor ene on chromosome 17q21. Genomics, 1999, 59(1):59-65.
24. Oberstein A, Jeffrey PD, Shi Y. Crystal structure of the Bcl-XL-Beclin 1 peptide complex:Beclin 1 is a novel BH3-only protein. J Biol Chem, 2007, 282(17):13123-13132.
25. 钱学茜, 万小云.子宫内膜癌发生过程中自噬活性改变的初步探讨.现代妇产科进展, 2008, 17(7):517-519.

Chinese Journal of Evidence-Based Medicine

Correlation between Beclin1 Protein Expression and Cervical Cancer Risk: A Meta-Analysis

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