Effects of Short-term Fertilization versus Conventional Fertilization in Vitro Fertilization Cycles: A Meta-Analysis_Chinese Journal of Evidence-Based Medicine

Authors：

 JIANQi-liang ¹ ,  WANGFang ¹ , DINGNan ² , DUHong ¹ , HUANGShan ¹

1. Department of Reproductive Medicine, Second Hospital of Lanzhou University, Lanzhou 730030, China;
2. Second Medical College of Lanzhou University, Lanzhou 730030, China;

Corresponding author：

WANGFang, Email: wangfang4323@126.com

Keywords：

In vitro fertilization-embryo transfer; Short-term fertilization; Conventional fertilization; Systematic review; Meta-analysis; Randomized controlled trial

DOI：

10.7507/1672-2531.20140240

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To systematically review the clinical effects of short-term and conventional fertilization for vitro fertilization-embryo transfer (IVF-ET). Methods Randomized controlled trials (RCTs) about the clinical effects of short-term fertilization versus conventional fertilization for IVF-ET were searched in PubMed, The Cochrane Library (Issue 8, 2014), CBM, CNKI, WanFang Data and VIP from inception to August 2014. Two reviewers independently screened literature according to the inclusion and exclusion criteria, extracted data, and assessed methodological quality of included studies. Then meta-analysis was performed using RevMan 5.2 software. Results A total of six RCTs involving 1 373 patients were finally included. The results of meta-analysis indicated that:short-term fertilization was superior to conventional fertilization in increasing high quality embryo rates (OR=1.42, 95%CI 1.18 to 1.70, P=0.000 2) as well as clinical pregnancy rates (OR=1.67, 95%CI 1.33 to 2.09, P < 0.000 01). However, the two groups were alike in fertilization rates, polyspermy rates, and miscarriage rates. Conclusion Current evidence indicates that short-term fertilization is superior to conventional fertilization in increasing high quality embryo rates as well as clinical pregnancy rates. Due to limited quality and quantity of the included studies, the above conclusion should be verified by conducting more large-scale, high quality RCTs with long-term follow-up.

Citation： JIANQi-liang, WANGFang, DINGNan, DUHong, HUANGShan. Effects of Short-term Fertilization versus Conventional Fertilization in Vitro Fertilization Cycles: A Meta-Analysis. Chinese Journal of Evidence-Based Medicine, 2014, 14(12): 1504-1509. doi: 10.7507/1672-2531.20140240 Copy

1.	李力.实用生殖医学.北京:人民卫生出版社, 2012:209.
2.	李晓红, 王蒿, 张晓, 等.短时受精与卵胞浆内单精子注射的临床应用研究.中华泌尿外科杂志, 2004, 25(7):485-487.
3.	黄国宁, 韩伟, 国玉蕊.短时受精与"搭桥受精".生殖医学杂志, 2009, 18(3):180-183.
4.	Dirnfeld M, Bider D, Koifman M, et al. Shortened exposure of oocytes to spermatozoa improve in vitro fertilization outcome:a Prospective randomized controlled study. Hum Reprod, 1999, 14(11):2562-2564.
5.	Barraud-Lange V, Sifer C, Pocate K, et al. Short gamete coincubation duringinvitro fertilization decreases the fertilization rateand does not imProveembryo quality:aprospectiveauto controlled study. J Assist Reprod Genet, 2008, 25(7):305-310.
6.	张宁媛, 孙海翔, 胡娅莉, 等.短时受精联合早期补救性卵细胞胞质内单精子注射在完全受精失败周期的临床应用, 中华男科学杂志, 2009, 15(6):538-541.
7.	李豫峰, 镇春, 何巧花, 等.体外受精-胚胎移植患者妊娠率与年龄及多精受精关系的研究.中国优生与遗传杂志, 2008, 16(1):102-103.
8.	李媛.人类辅助生殖实验技术.北京:科学出版社, 2008:100.
9.	Higgins JP, Green S. Cochrane Handbook for Systematic Reviews of Interventions (Version 5.1.0).[cited 2012 Jan 5]. Available at: http://www.cochrane-handbook.org.
10.	Gianaroli L, Cristina Magli M, Magli MC, et al. Reducing the time of sperm-oocyte interaction in human in·vitro fertilization improves the implantation rate. Hum ReProd, 1996, 11(1):166-171.
11.	Kattera S, Chen C. Short coincubation of gametes in in-vitro fertilization improves implantation and Pregnancyrates:a prospective, randomized, controlled study. Fertil Steril, 2003, 80(4):1017-1021.
12.	刁英, 杨智敏, 谭兵兵, 等.短时受精在体外受精-胚胎移植治疗周期中的应用价值.中国妇幼保健, 2010, 25(15):2104-2106.
13.	李瑞岐, 王文军, 黄丽丽, 等.短时受精对体外受精妊娠结局的影响.中山大学学报, 2012, 33(5):683-686.
14.	张若鹏, 邵华, 张丽蓉, 等.短时受精对体外受精-胚胎移植胚胎质量与妊娠率的影响.生殖医学杂志, 2004, 13(1):44-46.
15.	Harraway C, Berger NG, Dubin NH, et al. Semen PH in patients with normal versus abnormal sperm characteristics. Am J Obstet Gyneco, 2000, 182:1045-1047.
16.	罗丽兰.不孕与不育.北京:人民卫生出版社, 1999:418-423.
17.	Lundqvist M, Johansson U, Lundkvist O, et al. Reducing the time of co-incubation of gametes in human in vitro fertilization has no beneficial effect. Reprod Biomed Online, 2001, 3(1):21-24.
18.	Enkhmaa D, Kasai T, Hoshi K. Long-time exposure of mouse embryos to the sperm produces high levels of reactive oxygen species in culture medium and relates to poor embryo development. Reprod Domest Anim, 2009, 44(4):634-637.
19.	Navarro C, LoPez E, Stone B, et al. Short coincubation of sperm with oocytes is associated with an increased incidence of Polyspermy in human in vitro fertilization (IVF). Fertil Steril, 2004, 82(Suppl 2):S325-S325.
20.	乐杰, 主编.妇产科学.第5版, 北京:人民卫生出版社, 2002:34-35.
21.	李蕾, 李尚为, 靳松, 等.应用颗粒细胞部分剥除法提高行体外受精-胚胎移植后胚胎质量及临床妊娠率的研究.中华妇产科杂志, 2006, 41(3):201-202.
22.	Bedaiwy MA, Falcone T, Mohamed MS, et al. Differential growth of human embryos in vitro:role of reactive oxygen species. Fertil Steril, 2004, 82(3):593-600.
23.	MenezoY, Barak Y. Comparison between day 2 embryos obtained either from ICSI or Resulting from short insemination IVF:influence of maternal age. Hum Reprod, 2000, 15(9):1776-1780.
24.	Shau PL, Robertkuo KL, Jin TS, et al. The effects of brief gamete coincubation in human in vitro fertilization. J Assist Reprod Gen, 2000, 17(6):344-348.
25.	Swenson K, Check JH, Summers D, et al. A randomized study comparing the effect of standard verse short incubation of sperm and occyte on subsequent pregnancy and implantation rates following in vitro fertilization embryo transfer. Arch Androl, 2000, 45(1):73-76.
26.	柴三明, 刘珍, 倪亚莉, 等.体外受精中精卵孵育时间对结局的影响.中国优生与遗传杂志, 2006, 14(10):83-84.
27.	Dirnfeld M, Shiloh H, Brder E. et al. A prospective randomized controlled study of the effect of short coincubation of gametes during insemination on Zona pellucida thickness. Gynecol Endocrinol, 2003, 17(5):397-403.
28.	肖劲松, 陈双陨, 张春莲, 等.促性腺激素释放激素拮抗剂在体外受精-胚胎移植中有效性的系统性评价.中国循证医学杂志, 2010, 10(12):1432-1438.

1. 李力.实用生殖医学.北京:人民卫生出版社, 2012:209.
2. 李晓红, 王蒿, 张晓, 等.短时受精与卵胞浆内单精子注射的临床应用研究.中华泌尿外科杂志, 2004, 25(7):485-487.
3. 黄国宁, 韩伟, 国玉蕊.短时受精与"搭桥受精".生殖医学杂志, 2009, 18(3):180-183.
4. Dirnfeld M, Bider D, Koifman M, et al. Shortened exposure of oocytes to spermatozoa improve in vitro fertilization outcome:a Prospective randomized controlled study. Hum Reprod, 1999, 14(11):2562-2564.
5. Barraud-Lange V, Sifer C, Pocate K, et al. Short gamete coincubation duringinvitro fertilization decreases the fertilization rateand does not imProveembryo quality:aprospectiveauto controlled study. J Assist Reprod Genet, 2008, 25(7):305-310.
6. 张宁媛, 孙海翔, 胡娅莉, 等.短时受精联合早期补救性卵细胞胞质内单精子注射在完全受精失败周期的临床应用, 中华男科学杂志, 2009, 15(6):538-541.
7. 李豫峰, 镇春, 何巧花, 等.体外受精-胚胎移植患者妊娠率与年龄及多精受精关系的研究.中国优生与遗传杂志, 2008, 16(1):102-103.
8. 李媛.人类辅助生殖实验技术.北京:科学出版社, 2008:100.
9. Higgins JP, Green S. Cochrane Handbook for Systematic Reviews of Interventions (Version 5.1.0).[cited 2012 Jan 5]. Available at: http://www.cochrane-handbook.org.
10. Gianaroli L, Cristina Magli M, Magli MC, et al. Reducing the time of sperm-oocyte interaction in human in·vitro fertilization improves the implantation rate. Hum ReProd, 1996, 11(1):166-171.
11. Kattera S, Chen C. Short coincubation of gametes in in-vitro fertilization improves implantation and Pregnancyrates:a prospective, randomized, controlled study. Fertil Steril, 2003, 80(4):1017-1021.
12. 刁英, 杨智敏, 谭兵兵, 等.短时受精在体外受精-胚胎移植治疗周期中的应用价值.中国妇幼保健, 2010, 25(15):2104-2106.
13. 李瑞岐, 王文军, 黄丽丽, 等.短时受精对体外受精妊娠结局的影响.中山大学学报, 2012, 33(5):683-686.
14. 张若鹏, 邵华, 张丽蓉, 等.短时受精对体外受精-胚胎移植胚胎质量与妊娠率的影响.生殖医学杂志, 2004, 13(1):44-46.
15. Harraway C, Berger NG, Dubin NH, et al. Semen PH in patients with normal versus abnormal sperm characteristics. Am J Obstet Gyneco, 2000, 182:1045-1047.
16. 罗丽兰.不孕与不育.北京:人民卫生出版社, 1999:418-423.
17. Lundqvist M, Johansson U, Lundkvist O, et al. Reducing the time of co-incubation of gametes in human in vitro fertilization has no beneficial effect. Reprod Biomed Online, 2001, 3(1):21-24.
18. Enkhmaa D, Kasai T, Hoshi K. Long-time exposure of mouse embryos to the sperm produces high levels of reactive oxygen species in culture medium and relates to poor embryo development. Reprod Domest Anim, 2009, 44(4):634-637.
19. Navarro C, LoPez E, Stone B, et al. Short coincubation of sperm with oocytes is associated with an increased incidence of Polyspermy in human in vitro fertilization (IVF). Fertil Steril, 2004, 82(Suppl 2):S325-S325.
20. 乐杰, 主编.妇产科学.第5版, 北京:人民卫生出版社, 2002:34-35.
21. 李蕾, 李尚为, 靳松, 等.应用颗粒细胞部分剥除法提高行体外受精-胚胎移植后胚胎质量及临床妊娠率的研究.中华妇产科杂志, 2006, 41(3):201-202.
22. Bedaiwy MA, Falcone T, Mohamed MS, et al. Differential growth of human embryos in vitro:role of reactive oxygen species. Fertil Steril, 2004, 82(3):593-600.
23. MenezoY, Barak Y. Comparison between day 2 embryos obtained either from ICSI or Resulting from short insemination IVF:influence of maternal age. Hum Reprod, 2000, 15(9):1776-1780.
24. Shau PL, Robertkuo KL, Jin TS, et al. The effects of brief gamete coincubation in human in vitro fertilization. J Assist Reprod Gen, 2000, 17(6):344-348.
25. Swenson K, Check JH, Summers D, et al. A randomized study comparing the effect of standard verse short incubation of sperm and occyte on subsequent pregnancy and implantation rates following in vitro fertilization embryo transfer. Arch Androl, 2000, 45(1):73-76.
26. 柴三明, 刘珍, 倪亚莉, 等.体外受精中精卵孵育时间对结局的影响.中国优生与遗传杂志, 2006, 14(10):83-84.
27. Dirnfeld M, Shiloh H, Brder E. et al. A prospective randomized controlled study of the effect of short coincubation of gametes during insemination on Zona pellucida thickness. Gynecol Endocrinol, 2003, 17(5):397-403.
28. 肖劲松, 陈双陨, 张春莲, 等.促性腺激素释放激素拮抗剂在体外受精-胚胎移植中有效性的系统性评价.中国循证医学杂志, 2010, 10(12):1432-1438.

Chinese Journal of Evidence-Based Medicine

Effects of Short-term Fertilization versus Conventional Fertilization in Vitro Fertilization Cycles: A Meta-Analysis

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content