Analysis of iron regulated locus in <i>Klebsiella Pneumoniae</i> isolates from blood culture of liver abscess patients in Sichuan Provincial People’s Hospital _Chinese Journal of Evidence-Based Medicine

Authors：

ZHANG Jie ,  YU Hua

Department of Clinical Laboratory Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu, 610072, P.R.China;

Corresponding author：

YU Hua, Email: yvhua2002@163.com

Keywords：

Klebsiella pneumoniae; Iron regulated locus; Liver abscess; Risk factor

DOI：

10.7507/1672-2531.201708029

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the iron regulated locus in Klebsiella pneumoniae isolates from blood culture of liver abscess patients in Sichuan Provincial People’s Hospital. Methods From January to December of 2015, a total of 10 isolates of Klebsiella pneumoniae were collected from blood culture of liver abscess patients from Sichuan Provincial People’s Hospital. The genomic DNA was extracted to identify the genes of iroB, iroC, and iroD by PCR, and data was further analyzed by Graphpad Prism 5 software. Results Among the 10 Klebsiella pneumoniae clinical strains, 9 strains were iroB positive strains, 9 strains were iroC positive strains, and 10 strains were iroD positive strains, 9 strains were iroB/C/D triple positive. Conclusion The current study suggests that the frequency of triple positive of iroB/C/D in Klebsiella pneumoniae is high in isolates from liver abscess patients, the triple positive of iroB/C/D may contribute to liver abscess.

Citation： ZHANG Jie, YU Hua. Analysis of iron regulated locus in Klebsiella Pneumoniae isolates from blood culture of liver abscess patients in Sichuan Provincial People’s Hospital . Chinese Journal of Evidence-Based Medicine, 2017, 17(8): 907-909. doi: 10.7507/1672-2531.201708029 Copy

1.	Liu Y, Wang JY, Jiang W. An increasing prominent disease of Klebsiella pneumoniae liver abscess: etiology, diagnosis, and treatment. Gastroenterol Res Pract, 2013, 2013: 258514.
2.	Nairz M, Haschka D, Demetz E, et al. Iron at the interface of immunity and infection. Front Pharmacol, 2014, 5: 152.
3.	Cassat JE, Skaar EP. Iron in infection and immunity. Cell Host Microbe, 2013, 13(5): 509-519.
4.	Sorsa LJ, Dufke S, Heesemann J, et al. Characterization of an iroBCDEN gene cluster on a transmissible plasmid of uropathogenic Escherichia coli: evidence for horizontal transfer of a chromosomal virulence factor. Infect Immun, 2003, 71(6): 3285-3293.
5.	Bäumler AJ, Norris TL, Lasco T, et al. IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica. J Bacteriol, 1998, 180(6): 1446-1453.
6.	Caza M, Lépine F, Milot S, et al. Specific roles of the iroBCDEN genes in virulence of an avian pathogenic Escherichia coli O78 strain and in production of salmochelins. Infect Immun, 2008, 76(8): 3539-3549.
7.	Porcheron G, Garénaux A, Proulx J, et al. Iron, copper, zinc, and manganese transport and regulation in pathogenic enterobacteria: correlations between strains, site of infection and the relative importance of the different metal transport systems for virulence. Front Cell Infect Microbiol, 2013, 3: 90.
8.	Holden VI, Bachman MA. Diverging roles of bacterial siderophores during infection. Metallomics, 2015, 7(6): 986-995.
9.	Hsieh PF, Lin TL, Lee CZ, et al. Serum-induced iron-acquisition systems and TonB contribute to virulence in Klebsiella pneumoniae causing primary pyogenic liver abscess. J Infect Dis, 2008, 197(12): 1717-1727.
10.	Hsu CR, Lin TL, Chen YC, et al. The role of Klebsiella pneumoniae rmpA in capsular polysaccharide synthesis and virulence revisited. Microbiology, 2011, 157(Pt 12): 3446-3457.
11.	Ye M, Tu J, Jiang J, et al. Clinical and genomic analysis of liver abscess-causing klebsiella pneumoniae identifies new liver abscess-associated virulence genes. Front Cell Infect Microbiol, 2016, 6: 165.
12.	吴华, 李东冬, 王京, 等. 肺炎克雷伯杆菌肝脓肿的临床及微生物特征分析. 中华医学杂志, 2015, 95(40): 3259-3263.
13.	陈祥, 刘静, 高崧, 等. 禽病原性大肠杆菌 iro 和 tsh 基因缺失株的构建. 生物工程学报, 2008, 24(3): 401-408.
14.	Schmidt PJ. Regulation of iron metabolism by hepcidin under conditions of inflammation. J Biol Chem, 2015, 290(31): 18975-18983.
15.	刘强, 曹锋, 王亚军, 等. 细菌性肝脓肿经皮穿刺置管引流与细针抽吸疗效比较. 中国普外基础与临床杂志, 2010, 17(10): 1083-1086.

1. Liu Y, Wang JY, Jiang W. An increasing prominent disease of Klebsiella pneumoniae liver abscess: etiology, diagnosis, and treatment. Gastroenterol Res Pract, 2013, 2013: 258514.
2. Nairz M, Haschka D, Demetz E, et al. Iron at the interface of immunity and infection. Front Pharmacol, 2014, 5: 152.
3. Cassat JE, Skaar EP. Iron in infection and immunity. Cell Host Microbe, 2013, 13(5): 509-519.
4. Sorsa LJ, Dufke S, Heesemann J, et al. Characterization of an iroBCDEN gene cluster on a transmissible plasmid of uropathogenic Escherichia coli: evidence for horizontal transfer of a chromosomal virulence factor. Infect Immun, 2003, 71(6): 3285-3293.
5. Bäumler AJ, Norris TL, Lasco T, et al. IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica. J Bacteriol, 1998, 180(6): 1446-1453.
6. Caza M, Lépine F, Milot S, et al. Specific roles of the iroBCDEN genes in virulence of an avian pathogenic Escherichia coli O78 strain and in production of salmochelins. Infect Immun, 2008, 76(8): 3539-3549.
7. Porcheron G, Garénaux A, Proulx J, et al. Iron, copper, zinc, and manganese transport and regulation in pathogenic enterobacteria: correlations between strains, site of infection and the relative importance of the different metal transport systems for virulence. Front Cell Infect Microbiol, 2013, 3: 90.
8. Holden VI, Bachman MA. Diverging roles of bacterial siderophores during infection. Metallomics, 2015, 7(6): 986-995.
9. Hsieh PF, Lin TL, Lee CZ, et al. Serum-induced iron-acquisition systems and TonB contribute to virulence in Klebsiella pneumoniae causing primary pyogenic liver abscess. J Infect Dis, 2008, 197(12): 1717-1727.
10. Hsu CR, Lin TL, Chen YC, et al. The role of Klebsiella pneumoniae rmpA in capsular polysaccharide synthesis and virulence revisited. Microbiology, 2011, 157(Pt 12): 3446-3457.
11. Ye M, Tu J, Jiang J, et al. Clinical and genomic analysis of liver abscess-causing klebsiella pneumoniae identifies new liver abscess-associated virulence genes. Front Cell Infect Microbiol, 2016, 6: 165.
12. 吴华, 李东冬, 王京, 等. 肺炎克雷伯杆菌肝脓肿的临床及微生物特征分析. 中华医学杂志, 2015, 95(40): 3259-3263.
13. 陈祥, 刘静, 高崧, 等. 禽病原性大肠杆菌 iro 和 tsh 基因缺失株的构建. 生物工程学报, 2008, 24(3): 401-408.
14. Schmidt PJ. Regulation of iron metabolism by hepcidin under conditions of inflammation. J Biol Chem, 2015, 290(31): 18975-18983.
15. 刘强, 曹锋, 王亚军, 等. 细菌性肝脓肿经皮穿刺置管引流与细针抽吸疗效比较. 中国普外基础与临床杂志, 2010, 17(10): 1083-1086.

Previous Article
Pathogens causing bloodstream infection after general surgery in infant and young children patients
Next Article
Development the short version of elderly disability assessment scale

Chinese Journal of Evidence-Based Medicine

Analysis of iron regulated locus in Klebsiella Pneumoniae isolates from blood culture of liver abscess patients in Sichuan Provincial People’s Hospital

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content