The dynamic expression of Robo3 in the hippocampus of the lithium-pilocarpine induced rat model_Journal of Epilepsy

Authors：

 ZHOUYi , LUOZhaohui , HEXinghui ,  XIAOBo

Department of Neurology, Xiang Ya Hospital of Central South University, Changsha 410000, Hunan, China;

Corresponding author：

XIAOBo, Email: xiaobo_xy@126.com

Keywords：

Temporal lobe epilepsy; Robo3; Axon guidance molecule; Rats

DOI：

10.7507/2096-0247.20160007

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To characterize the dynamic expression of Robo3 in the rat model of temporal lobe epilepsy(TLE), and assess the potential contribution of Robo3 to epileptogenesis. Methods Male Sprague-Dawley (SD) rats were randomly divided into the control group (n=6) and the experimental groups (n=30, 6 per group). The experimental groups were injected intraperitoneally (i.p.) with an aqueous solution of lithium-pilocarpine, and sacrificed at different time points (1, 7, 14, 30 and 60 days) following the seizure. The control group was i.p. with 0.9% sodium chloride instead of pilocarpine. Quantitative real-time PCR were used to detected the mRNA expression of Robo3 and Western bolt were used to detected the protein expression of Robo3. Results Quantitative real-time PCR showed that the expression of Robo3 were significantly lower in the rat temporal lobe tissues of the latent and the chronic period group as compared with the controls(P < 0.05), but no significant differences were identified between the acute period group and the controls(P > 0.05). Western blot showed that the protein expression of Robo3 were significantly lower in the rat temporal lobe tissues of the latent and the chronic period group as compared with the controls(P < 0.05), no significant differences were identified between the acute period group and the controls(P > 0.05). Conclusions Robo3 may be involved in the pathogenesis of temporal lobe epilepsy.

Citation： ZHOUYi, LUOZhaohui, HEXinghui, XIAOBo. The dynamic expression of Robo3 in the hippocampus of the lithium-pilocarpine induced rat model. Journal of Epilepsy, 2016, 2(1): 34-37. doi: 10.7507/2096-0247.20160007 Copy

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6.	Wong K, Ren XR, Huang YZ, et al. Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway. Cell, 2001, 107(2): 209-221.
7.	Camurri L, Mambetisaeva E, Davies D, et al. Evidence for the existence of two Robo3 isoforms with divergent biochemical properties. Molecular and Cellular Neurosciences, 2005, 30(4): 485-493.
8.	路蝉伊, 刘立雄, 张云峰.癫痫发病机制的研究进展.解放军医学院学报, 2014, 35(8): 876-881.
9.	Fang M, Xi ZQ, Wu Y, et al. A new hypothesis of drug refractory epilepsy: neural network hypothesis. Medical Hypotheses, 2011, 76(6): 871-876.
10.	Yuan SS, Cox LA, Dasika GK, et al. Cloning and functional studies of a novel gene aberrantly expressed in RB-deficient embryos. Developmental Biology, 1999, 207(1): 62-75.
11.	Chen Z, Gore BB, Long H, et al. Alternative splicing of the Robo3 axon guidance receptor governs the midline switch from attraction to repulsion. Neuron, 2008, 58(3): 325-332.
12.	Rajagopalan S, Vivancos V, Nicolas E, et al. Selecting a longitudinal pathway: Robo receptors specify the lateral position of axons in the Drosophila CNS. Cell, 2000, 103(7): 1033-1045.
13.	Rajagopalan S, Nicolas E, Vivancos V, et al. Crossing the midline: roles and regulation of Robo receptors. Neuron, 2000, 28(3): 767-777.
14.	Barber M, Di Meglio T, Andrews WD, et al. The role of Robo3 in the development of cortical interneurons. Cerebral Cortex, 2009, 19(Suppl 1): 22-31.
15.	Li X, Chen Y, Liu Y, et al. Structural basis of Robo proline-rich motif recognition by the srGAP1 Src homology 3 domain in the Slit-Robo signaling pathway. The Journal of Biological Chemistry, 2006, 281(38): 28430-28437.
16.	Major DL, Brady-Kalnay SM. Rho GTPases regulate PTPmu-mediated nasal neurite outgrowth and temporal repulsion of retinal ganglion cell neurons. Molecular and Cellular Neurosciences, 2007, 34(3): 453-467.

1. Thurman DJ, Beghi E, Begley CE, et al. Standards for epidemiologic studies and surveillance of epilepsy. Epilepsia, 2011, 52(Suppl 7): 2-26.
2. Morimoto K, Fahnestock M, Racine RJ. Kindling and status epilepticus models of epilepsy: rewiring the brain. Progress in Neurobiology, 2004, 73(1): 1-60.
3. Fang M, Liu GW, Pan YM, et al. Abnormal expression and spatiotemporal change of Slit2 in neurons and astrocytes in temporal lobe epileptic foci: a study of epileptic patients and experimental animals. Brain Research, 2010, 1324(4):14-23.
4. Dickson BJ. Rho GTPases in growth cone guidance. Current Opinion in Neurobiology, 2001, 11(1): 103-110.
5. Ghose A, Van Vactor D. GAPs in Slit-Robo signaling. Bio Essays, 2002, 24(5): 401-404.
6. Wong K, Ren XR, Huang YZ, et al. Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway. Cell, 2001, 107(2): 209-221.
7. Camurri L, Mambetisaeva E, Davies D, et al. Evidence for the existence of two Robo3 isoforms with divergent biochemical properties. Molecular and Cellular Neurosciences, 2005, 30(4): 485-493.
8. 路蝉伊, 刘立雄, 张云峰.癫痫发病机制的研究进展.解放军医学院学报, 2014, 35(8): 876-881.
9. Fang M, Xi ZQ, Wu Y, et al. A new hypothesis of drug refractory epilepsy: neural network hypothesis. Medical Hypotheses, 2011, 76(6): 871-876.
10. Yuan SS, Cox LA, Dasika GK, et al. Cloning and functional studies of a novel gene aberrantly expressed in RB-deficient embryos. Developmental Biology, 1999, 207(1): 62-75.
11. Chen Z, Gore BB, Long H, et al. Alternative splicing of the Robo3 axon guidance receptor governs the midline switch from attraction to repulsion. Neuron, 2008, 58(3): 325-332.
12. Rajagopalan S, Vivancos V, Nicolas E, et al. Selecting a longitudinal pathway: Robo receptors specify the lateral position of axons in the Drosophila CNS. Cell, 2000, 103(7): 1033-1045.
13. Rajagopalan S, Nicolas E, Vivancos V, et al. Crossing the midline: roles and regulation of Robo receptors. Neuron, 2000, 28(3): 767-777.
14. Barber M, Di Meglio T, Andrews WD, et al. The role of Robo3 in the development of cortical interneurons. Cerebral Cortex, 2009, 19(Suppl 1): 22-31.
15. Li X, Chen Y, Liu Y, et al. Structural basis of Robo proline-rich motif recognition by the srGAP1 Src homology 3 domain in the Slit-Robo signaling pathway. The Journal of Biological Chemistry, 2006, 281(38): 28430-28437.
16. Major DL, Brady-Kalnay SM. Rho GTPases regulate PTPmu-mediated nasal neurite outgrowth and temporal repulsion of retinal ganglion cell neurons. Molecular and Cellular Neurosciences, 2007, 34(3): 453-467.

Journal of Epilepsy

The dynamic expression of Robo3 in the hippocampus of the lithium-pilocarpine induced rat model

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