Dynamic changes of microvessels in the hippocampal CA3 area in a mouse model of temporal lobe epilepsy_Journal of Epilepsy

Authors：

 XIEYuanyuan ¹ ,  SHUYi ² , LONGHongyu ¹ , XIAOBo ¹

1. Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410008, China;
2. Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, 410011, China;

Corresponding author：

SHUYi, Email: shuyi_1986@aliyun.com

Keywords：

Temporal lobe epilepsy; Mice; Hippocampus; Microvessel; Pilocarpine

DOI：

10.7507/2096-0247.20160058

Video：

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Objective To explore the dynamic changes of microvessels in the hippocampal CA3 area in mice model of temporal lobe epilepsy (TLE) induced by pilocarpine. Methods Eighteen health SPF male C57BL/6 mice were randomly divided into control group and status epilepticus (SE) group. The SE group was subdivided into three groups:SE-7 days, SE-28 days and SE-56 days. SE was induced by intraperitoneal injection of pilocarpine. And immunohistochemical staining was used to detected the localization of platelet endothelial cell adhesion molecule-1 (PECAM-1). Results In the control group, PECAM-1 labeled microvessels arranged in a layered structure, and the microvessel of the orient layer was most prominent. After SE, the microvessels started to form an unorganized vascular plexus and appeared fibrous and fragmented, which was prominent at SE-28 days. Furthermore, the microvessels density increased the top at SE-28 days compared to the control (P < 0.001). Conclusion The angiogenesis exists during the hippocampus formation in the mice model of TLE induced by pilocarpine, which could direct a new explanation for TLE formation and development.

Citation： XIEYuanyuan, SHUYi, LONGHongyu, XIAOBo. Dynamic changes of microvessels in the hippocampal CA3 area in a mouse model of temporal lobe epilepsy. Journal of Epilepsy, 2016, 2(4): 329-332. doi: 10.7507/2096-0247.20160058 Copy

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8.	Duijvestijn AM, van Goor H, Klatter F, et al. Antibodies defining rat endothelial cells:RECA-1, a pan-endothelial cell-specific monoclonal antibody. Lab Invest, 1992, 66(4):459-466.
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11.	Kastanauskaite A, Alonso-Nanclares L, Blazquez-Llorca L, et al. Alterations of the microvascular network in sclerotic hippocampi from patients with epilepsy. J Neuropathol Exp Neurol, 2009, 68(8):939-950.
12.	Alonso-Nanclares L, Defelipe J. Alterations of the microvascular network in the sclerotic hippocampus of patients with temporal lobe epilepsy. Epilepsy Behav, 2014, 38(5):48-52.

1. Pitkänen A, Lukasiuk K. Molecular and cellular basis of epileptogenesis in symptomatic epilepsy. Epilepsy Behav, 2009, 14(Suppl 1):16-25.
2. Morin-Brureau M, Rigau V, Lerner-Natoli M. Why and how to target angiogenesis in focal epilepsy. Epilepsia, 2012, 53(Suppl 6):64-68.
3. Hunsberger JG, Bennett AH, Selvanayagam E, et al. Gene profiling the response to kainic acid induced seizures. Brain Res Mol Brain Res, 2005, 141(1):95-112.
4. Wang D, Song X, Wang Y, et al. Gene expression profile analysis in epilepsy by using the partial least squares method. Scientific World Journal, 2014, 20(5):731091.
5. Morin-Brureau M, Lebrun A, Rousset MC, et al. Epileptiform activity induces vascular remodeling and zonula occludens 1 downregulation in organotypic hippocampal cultures:role of VEGF signaling pathways. J Neurosci, 2011, 31(29):10677-10688.
6. Ndode-Ekane XE, Hayward N, Grohn O, et al. Vascular changes in epilepsy:functional consequences and association with network plasticity in pilocarpine-induced experimental epilepsy. Neuroscience, 2010, 166(1):312-332.
7. Maolood N, Meister B. Protein components of the blood-brain barrier (BBB) in the brainstem area postrema-nucleus tractus solitarius region. J Chem Neuroanat, 2009, 37(3):182-195.
8. Duijvestijn AM, van Goor H, Klatter F, et al. Antibodies defining rat endothelial cells:RECA-1, a pan-endothelial cell-specific monoclonal antibody. Lab Invest, 1992, 66(4):459-466.
9. Rigau V, Morin M, Rousset MC, et al. Angiogenesis is associated with blood-brain barrier permeability in temporal lobe epilepsy. Brain, 2007, 130(Pt 7):1942-1956.
10. Nicoletti JN, Shah SK, McCloskey DP, et al. Vascular endothelial growth factor is up-regulated after status epilepticus and protects against seizure-induced neuronal loss in hippocampus. Neuroscience, 2008, 151(1):232-241.
11. Kastanauskaite A, Alonso-Nanclares L, Blazquez-Llorca L, et al. Alterations of the microvascular network in sclerotic hippocampi from patients with epilepsy. J Neuropathol Exp Neurol, 2009, 68(8):939-950.
12. Alonso-Nanclares L, Defelipe J. Alterations of the microvascular network in the sclerotic hippocampus of patients with temporal lobe epilepsy. Epilepsy Behav, 2014, 38(5):48-52.

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Dynamic changes of microvessels in the hippocampal CA3 area in a mouse model of temporal lobe epilepsy

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