脑衰反应调节蛋白2的表达与颞叶癫痫关系的研究_Journal of Epilepsy

Authors：

张文霞 , 余洋 , 林卫红 ,  孟红梅

吉林大学白求恩第一医院神经内科 (长春 130021);

Corresponding author：

孟红梅, Email: hongmeiyp@126.com

Keywords：

脑衰反应调节蛋白-2; 颞叶癫痫; 神经元微管; 轴突; 树突

DOI：

10.7507/2096-0247.20170020

Video：

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颞叶癫痫 (Temporal lobe epilepsy, TLE) 是最常见的癫痫类型，大部分为药物难治性癫痫，最主要的病理特征是海马苔藓纤维出芽和突触重塑，是目前研究的热点。国内外许多学者运用比较蛋白质组学技术，通过比较TLE患者或动物模型组与对照组的差异蛋白质，发现脑衰反应调节蛋白2(CRMP-2) 的表达下调最显著，表明CRMP-2与TLE密切相关。CRMP-2属于细胞质磷蛋白，高表达于中枢神经系统，尤其是神经元和少突胶质细胞。通过对CRMP-2表达信号通路作用机制的相关性研究，CRMP-2在经典通路中通过影响神经元微管的合成从而调节轴突和树突的生长，推测CRMP-2在TLE海马神经元损伤的作用是通过PTEK/P13K/Akt/GSK-3β/CRMP-2信号通路进行的，可揭示TLE的发病机制及为进一步的临床治疗提供新的作用靶点。

Citation： 张文霞, 余洋, 林卫红, 孟红梅. 脑衰反应调节蛋白2的表达与颞叶癫痫关系的研究. Journal of Epilepsy, 2017, 3(2): 137-140. doi: 10.7507/2096-0247.20170020 Copy

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2.	林华, 吴立文, 黄远桂, 等.海马苔藓纤维出芽及突触重组与颞叶癫痫的相关性研究.中华医学杂志, 2007, 87(5) : 341-344.
3.	Xue Y, Xie N, Cao L, et al. Diazoxide preconditioning against seizure-induced oxidative injury is via the PI3K/Akt pathway in epileptic rat. Neurosci Lett, 2011, 495(2): 130-134.
4.	Biagini G, Baldelli E, Longo D, et al. Proepileptic influence of a focal vascularlesion affecting entorhinal cortex-CA3 connections after status epilepticus. J Neuropathol Exp Neurol, 2008, 67(7): 687-701.
5.	Mizushima N, Komatsu M. Autophagy: renovation of cells and tissues. Cell, 2011, 147 (4): 728-741.
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10.	Rogawski MA, Tofighy A, White HS, et al. Current understanding of the mechanism of action of the antiepileptic drug lacosamide. Epilepsy Research, 2015, 110(2): 189-205.
11.	Wilson SM, Moutal A, Melemedjian OK, et al. The functionalized amino acid (S)-Lacosamide Subverts CRMP2-mediated tubulin polymerization to prevent constitutive and activity-dependent increase in neurite outgrowth. Front Cell Neurosci, 2014, 24(8): 196-198.
12.	Inagaki N, Chihara K, Arimura N, et al. CRMP-2 induces axons in cultured hippocampal neurons. Nat Neurosci, 2001, 4(8): 781-782.
13.	Arimura N, Menager C, Kawano Y, et al. Phosphorylation by rho kinase regulates CRMP-2 activity in growth cones. Mol Cell Biol, 2005, 25(22): 9973-9984.
14.	Akama K, Horikoshi T, Nakayama T, et al. Proteomic identification of differentially expressed genes in neural stem cells and neurons differentiated from embryonic stem cells of cynomolgus monkey (Macaca fascicularis) in vitro. Biochimica Biophysica Acta, 2011, 1814(2): 265-276.
15.	Soutar MP, Thornhill P, Cole AR. Increased CRMP2 phosphorylation is observed in Alzheimers disease: does this tell us anything about disease development. Curr Alz Res, 2009, 6(1): 269-278.
16.	Vchida Y, Ohshima T, Sasaki Y. Semaphorin 3A signaling is mediated via sequential Cdk5 and GSK3β phosphorylation of CRMP2: implication of common phosphorylating mechanism underling axon guidance and alzheimer′s desease. Genas Cells, 2005, 10(3): 165-179.
17.	Soutar MP, Thornhill P, Cole AR. Increased CRMP2 Phosphorylation is observed in Alzheimer′s disease:dose this tell us anything about disease development. Curr Alz Res, 2009, 6(1): 269-278.
18.	Yoshimura T, Kawano Y, Arimura N. GSK-3β regulates phosphorylation of CRMP-2 and neuronal polarity. Cell, 2005, 120(1): 137-149.
19.	Lykissas MG, Batistatou AK, Konstantinos A. The role of neurotrophins in axon guidance, growth and regeneration. Curr Neurovas Res, 2007, 4(1): 143-151.
20.	Arimura N, Kimura T, Nakamuta S. Anterograde transport of Trkβ in axons is mediated by direct interaction with slp1and rab2. Dev Cell, 2009, 15(7): 675-686.
21.	Kawano Y, Yoshimura T, Tsuboi D. CRMP-2 is involved in kinesin-1-development transport of the Swa-1/WAVE1 complex and axon formation. Mol Cell Biol, 2005, 25(3): 9920-9935.
22.	Rogemond V, Auger C, Giraudon P. Processing and nuclear localization of CRMP2 during brain development induce neurite outgrowth inhibition. J Biol Chem, 2008, 283(1): 14751-14761.
23.	Morita T, Sobue K. Specification of neuronal polarity mediated by mTOR-regulated local translation of CRMP2 and tau. J Biol Chem, 2009, 284(40): 27734-27745.
24.	Lee CY, Jaw T, Tseng HC. Lovastatin modulates glycogen synthase kinase-3β pathway and inhibits mossy fiber sprouting after pilocarpine-induced status epilepticus. PLoS ONE, 2012, 7(6): e38789.
25.	Garcia-Junco-Clemente P, Golshani P. PTEN: a master regulator of neuronal structure, function, and plasticity. Communicative & Integrative Biology, 2014, 7(1): e28358.
26.	Zhao J, Qu Y, Wu J, et al. PTEN inhibition prevents rat cortical neuron injury after hypoxia-ischemia. Neuroscience, 2013, 238 (15): 242-251.

1. Stern JM.Overview of evaluation and treatment guidelions for epilepsy. Curr Treat Options Neurol, 2009, 11(4): 273-284.
2. 林华, 吴立文, 黄远桂, 等.海马苔藓纤维出芽及突触重组与颞叶癫痫的相关性研究.中华医学杂志, 2007, 87(5) : 341-344.
3. Xue Y, Xie N, Cao L, et al. Diazoxide preconditioning against seizure-induced oxidative injury is via the PI3K/Akt pathway in epileptic rat. Neurosci Lett, 2011, 495(2): 130-134.
4. Biagini G, Baldelli E, Longo D, et al. Proepileptic influence of a focal vascularlesion affecting entorhinal cortex-CA3 connections after status epilepticus. J Neuropathol Exp Neurol, 2008, 67(7): 687-701.
5. Mizushima N, Komatsu M. Autophagy: renovation of cells and tissues. Cell, 2011, 147 (4): 728-741.
6. Wang Y, Qin ZH. Molecular and cellular mechanisms of excitotoxic neuronal death. Apoptosis, 2010, 7(5): 798-791.
7. Ip JP, Fu AK, Ip NY. CRMP2: functional roles in neural development and therapeutic potential in neurological diseases. Neuroscientist, 2014, 20(6): 589-598.
8. Tan F, Thiele CJ, Li Z. Collapsion response mediator proteins: potential diagnostic and prognostic biomarkers in cancers (revive). Oncol Lett, 2014, 7(1): 1333-1340.
9. Vincent P, Collette Y, Marignier R, et al. A role for the neuronal protein collapsin response mediator protein 2 in T lymphocyte polarization and migration. J Immunol, 2005, 175(11): 7650-7660.
10. Rogawski MA, Tofighy A, White HS, et al. Current understanding of the mechanism of action of the antiepileptic drug lacosamide. Epilepsy Research, 2015, 110(2): 189-205.
11. Wilson SM, Moutal A, Melemedjian OK, et al. The functionalized amino acid (S)-Lacosamide Subverts CRMP2-mediated tubulin polymerization to prevent constitutive and activity-dependent increase in neurite outgrowth. Front Cell Neurosci, 2014, 24(8): 196-198.
12. Inagaki N, Chihara K, Arimura N, et al. CRMP-2 induces axons in cultured hippocampal neurons. Nat Neurosci, 2001, 4(8): 781-782.
13. Arimura N, Menager C, Kawano Y, et al. Phosphorylation by rho kinase regulates CRMP-2 activity in growth cones. Mol Cell Biol, 2005, 25(22): 9973-9984.
14. Akama K, Horikoshi T, Nakayama T, et al. Proteomic identification of differentially expressed genes in neural stem cells and neurons differentiated from embryonic stem cells of cynomolgus monkey (Macaca fascicularis) in vitro. Biochimica Biophysica Acta, 2011, 1814(2): 265-276.
15. Soutar MP, Thornhill P, Cole AR. Increased CRMP2 phosphorylation is observed in Alzheimers disease: does this tell us anything about disease development. Curr Alz Res, 2009, 6(1): 269-278.
16. Vchida Y, Ohshima T, Sasaki Y. Semaphorin 3A signaling is mediated via sequential Cdk5 and GSK3β phosphorylation of CRMP2: implication of common phosphorylating mechanism underling axon guidance and alzheimer′s desease. Genas Cells, 2005, 10(3): 165-179.
17. Soutar MP, Thornhill P, Cole AR. Increased CRMP2 Phosphorylation is observed in Alzheimer′s disease:dose this tell us anything about disease development. Curr Alz Res, 2009, 6(1): 269-278.
18. Yoshimura T, Kawano Y, Arimura N. GSK-3β regulates phosphorylation of CRMP-2 and neuronal polarity. Cell, 2005, 120(1): 137-149.
19. Lykissas MG, Batistatou AK, Konstantinos A. The role of neurotrophins in axon guidance, growth and regeneration. Curr Neurovas Res, 2007, 4(1): 143-151.
20. Arimura N, Kimura T, Nakamuta S. Anterograde transport of Trkβ in axons is mediated by direct interaction with slp1and rab2. Dev Cell, 2009, 15(7): 675-686.
21. Kawano Y, Yoshimura T, Tsuboi D. CRMP-2 is involved in kinesin-1-development transport of the Swa-1/WAVE1 complex and axon formation. Mol Cell Biol, 2005, 25(3): 9920-9935.
22. Rogemond V, Auger C, Giraudon P. Processing and nuclear localization of CRMP2 during brain development induce neurite outgrowth inhibition. J Biol Chem, 2008, 283(1): 14751-14761.
23. Morita T, Sobue K. Specification of neuronal polarity mediated by mTOR-regulated local translation of CRMP2 and tau. J Biol Chem, 2009, 284(40): 27734-27745.
24. Lee CY, Jaw T, Tseng HC. Lovastatin modulates glycogen synthase kinase-3β pathway and inhibits mossy fiber sprouting after pilocarpine-induced status epilepticus. PLoS ONE, 2012, 7(6): e38789.
25. Garcia-Junco-Clemente P, Golshani P. PTEN: a master regulator of neuronal structure, function, and plasticity. Communicative & Integrative Biology, 2014, 7(1): e28358.
26. Zhao J, Qu Y, Wu J, et al. PTEN inhibition prevents rat cortical neuron injury after hypoxia-ischemia. Neuroscience, 2013, 238 (15): 242-251.

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