Effects of Tianjiang Niuhuang powder on the epileptic model of pilocarpine rats_Journal of Epilepsy

Authors：

YUAN Bin ¹ ,  BU Meiling ¹ , TIAN Jiayu ² , ZHANG Lei ¹ , SUN Yongan ²

1. Department of Neurology, HeZe Medical College affiliated hospital, Heze 274000, China;
2. Department of Neurology, The First Hospital of Beijing University, Beijing 100000, China;

Corresponding author：

BU Meiling, Email: 18653088558@163.com

Keywords：

Tianjiang Niuhuang Powder; Pilocarpine; Epileptic mode; Cognitive ability; Rats

DOI：

10.7507/2096-0247.20190033

Video：

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Objective To observe the effects of Tianjiang Niuhuang Powder on cognition, seizure and hippocampal formation in epileptic model of pilocarpine rats.Methods Fouty SD rats were selected in this study. They were randomly divided into four groups (each group n=10), including a blank control group (group A) and three pilocarpine epilepsy model groups. The model groups were divided into Tianjiang Niuhuang Powder treatment Group (group B), Sodium valproate (group C) and model control group (group D) according to the different processing methods. The seizures number of discharges in the EEG monitor, cognitive ability were observed on the 7th, 14th and 21st day, The pathological sections of the brain and the neuronal damage in the hippocampal CA1 area were observed after the rats were sacrificed.Results There was no significant difference in the number of spontaneous episodes, duration, and average number of discharges per minute between group B and group C (P>0.05). Compared with groups B and C, group D increased the number of episodes and increased duration. The number of discharges increased, the difference was statistically significant (P<0.05). Group A had no seizures. There were no significant differences in cognitive ability between groups A, B and C (P>0.05). Compared with group A, B and C, group D had poor cognitive ability (P<0.05). The number of surviving cells in the hippocampus CA1 region was significantly improved compared with the D group (P<0.05). Compared with the A group, the viable cells in the hippocampal CA1 region of the three experimental groups were significantly lower (P<0.05).Conclusions Tianjiang Niuhuang Powder can reduce seizures, improve cognition and effectively alleviate neuronal damage in hippocampal CA1 neurons in rats with epilepsy.

Citation： YUAN Bin, BU Meiling, TIAN Jiayu, ZHANG Lei, SUN Yongan. Effects of Tianjiang Niuhuang powder on the epileptic model of pilocarpine rats. Journal of Epilepsy, 2019, 5(3): 187-191. doi: 10.7507/2096-0247.20190033 Copy

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1. 姚俐. 川芎嗪抗小鼠实验性癫痫的研究. 广州: 南方科技大学, 硕士学位论文, 2013.
2. 洪震. 丙戊酸钠--癫痫治疗一线用药. 癫痫杂志, 2017, 3(3): 278-279.
3. Peng WF, Ding J, Li X, et al. N-methyl-d-aspartate receptor NR2B subunit involved in depression-like behaviours in lithium chloride-pilocarpine chronic rat epilepsy model. Epilepsy Res, 2016, 119: 77-85.
4. Pfammatter JA, Bergstorm RA, Wallace EP, et al. A predictive epilepsy index based on probabilistic classification of interictal spike waveforms. PLoS One, 2018, 13(11): e0207158.
5. Loddenkemper T, Talos DM, Cleary RT, et al. Subunit composition of glutamate and gamma-aminobutyric acid receptors in status epilepticus. Epilepsy Res, 2014, 108(4): 605-615.
6. Ghadiri T, Sharifzadeh M, Khodagholi M, et al. A novel traumatic brain injury model for induction of mild brain injury in rats. J Neurosci Methods, 2014, 233: 18-27.
7. Wan J, Wan H, Yang R, et al. Protective effect of Dandong Injection combined with Naoxintong Capsule on cerebral ishemia-reperfusion injury in rats. J Ethnopharmacol, 2018, 211: 348-357.
8. Tuor UI, Zhao Z, Barber PA, et al. Recurrent mild cerebral ischemia: enhanced brain injury following acute compared to subacute recurrence in the rat. BWC Neurosci, 2016, 17(1): 28.
9. Shih JJ, Whitlock JB, Chimato N, et al. Epilepsy treatment in adults and adolescents: Expert opinion, 2016. Epilepsy Behav, 69: 186-222.
10. Brodie MJ, Besag F, Ettinger AB, et al. Epilepsy, antiepileptic drugs, and aggression: an evidence-based review. Pharmacological Reviews, 2016, 68(3): 563-602.
11. Löscher W. Animal models of seizures and epilepsy: past, present, and future role for the discovery of antiseizure drugs. Neurochem Res, 2017, 42(7): 1873-1888.
12. Butler KM, Moody OA, Elisabeth S, et al. De novo variants in GABRA2 and GABRA5 alter receptor function and contribute to early-onset epilepsy. Brain, 2018, 141(8): 2392-2405.
13. Murase S. A new model for developmental neuronal death and excitatory/inhibitory balance in hippocampus. Mol Neurobiol, 2014, 49(1): 316-325.
14. Tse MT, Piantadosi PT, Floresco SB. Prefrontal cortical gamma-aminobutyric acid transmission and cognitive function: drawing links to schizophrenia from preclinical research. Biol Psychiatry, 2015, 77(11): 929-939.
15. Kang JQ. Defects at the crossroads of GABAergic signaling in generalized genetic epilepsies. Epilepsy Res, 2017, 137: 9-18.
16. Uchida T, Lossin C, Ihara Y, et al. Abnormal γ-aminobutyric acid neurotransmission in a Kcnq2 model of early onset epilepsy. Epilepsia, 2017, 58(8): 1430-1439.

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Effects of Tianjiang Niuhuang powder on the epileptic model of pilocarpine rats

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