Study of recombinant human erythropoietin on hippocampal proteomics in pentylenetetrazole-induced epileptic rats_Journal of Epilepsy

Authors：

ZHANG Wenjuan ¹ , WANG Guangwen ² ,  YANG Jianzhong ¹

1. Department of neurology, Shanxi Provincial Peoples, Hospital, Taiyuan 030012, China;
2. Department of Neurology, General Hospital of Taiyuan Iron & Steel CO., LTD. (TISCO), Taiyuan 030003, China;

Corresponding author：

YANG Jianzhong, Email: 1595021181@qq.com

Keywords：

Recombinant human erythropoietin; SD rats; Hippocampus; Proteomics

DOI：

10.7507/2096-0247.202207006

Video：

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Objective To study the differentially expressed proteins of recombinant human erythropoietin (r-HuEPO) in hippocampus of Pentetrazol (PTZ) -induced epileptic rats, and to provide a basis for exploring the pathogenesis of epilepsy and seeking new therapeutic targets. Methods Twelve 6~8-week-old Sprague Dawley rats that weighted 230~250 g were randomly divided into two groups: PTZ group, PTZ+ EPO group. The differential proteins of recombinant human EPO in hippocampus of pentylenetetrazole-induced epileptic rats were analyzed and identified by TMT technique based on mass spectrometry.Results 139 differentially expressed protein sites were detected in hippocampal tissues of epileptic rats, of which 55 were up-regulated and 84 down-regulated. Conclusion Recombinant human erythropoietin can inhibit many differentially expressed proteins in the hippocampus of pentaerythraze-induced eclampsia rats by upregulation of Isocitrate dehydrogenase (NADP), Reduced nicotinamide purine dinucleotide phosphate (NADPH), Thioredoxin reductase 2 mitochondrial (TrxR), reduce nerve cell damage.

Citation： ZHANG Wenjuan, WANG Guangwen, YANG Jianzhong. Study of recombinant human erythropoietin on hippocampal proteomics in pentylenetetrazole-induced epileptic rats. Journal of Epilepsy, 2022, 8(6): 529-534. doi: 10.7507/2096-0247.202207006 Copy

1.	Roland D Thijs, Rainer Surges, Terence J O'Brien, et al. Epilepsy in adults. Lancet, 2019, 393(10172): 689-701.
2.	张淑琴, 林卫红, 赵丽曼. 实验性癫痫不同脑区细胞凋亡的观察及意义. 中风与神经疾病杂志, 2002, 19(4): 205-207.
3.	Beng zon J, Mohapel P, Ekdahl CT, et al. Neuronal apoptosis after brief and prolonged seizures. Prog Brain Res, 2002, 135: 111-119.
4.	Stadtman ER. Protein oxidation in aging and age-related diseases. Ann N. Y. Acad. Sci, 2001, 928: 22-38.
5.	Wong-ekkabut J, Xu Z, Triampo W, et al. Effect of lipid peroxidation on the properties of lipid bilayers: a molecular dynamics study. Biophys J, 2007, 93: 4225-4236.
6.	Henshall DC, Murphy BM. Modulators of neuronal cell death in epilepsy. Curr Opin Pharmacol, 2008, 8(1): 75-81.
7.	宋修云, 胡金凤, 陈乃宏. 神经细胞凋亡与脑缺血疾病. 中国药理学通报, 2012, 28(3): 307-310. .
8.	王维平, 史志勤, 于江华等. 重组人红细胞生成素对癫痫持续状态大鼠海马p-Akt和caspase-9的影响及作用机制. 南方医科大学学报, 2010, 30(1): 64-69.
9.	史志勤, 王维平, 于江华, 等. 重组人红细胞生成素经PI3K/Akt途径对大鼠癫痫持续状态诱导的神经元凋亡的保护作用. 中国组织化学与细胞化学杂志, 2009, 18(1): 94-102.
10.	张思远, 蔡昕, 卢晓桦, 等. 新型H2S供体激活PI3K/Akt对癫痫大鼠脑内Bcl-2/Bax蛋白表达的影响. 华中科技大学学报(医学版), 2020, 49(3): 296-300.
11.	Singh A, Kukreti R, Saso L. Oxidative stress: a key modulator in neurodegenerative disease. Molecules, 2019, 24(8): 1583.
12.	Waldbaum S, Patel M. Mitochondrial oxidative stress in temporal lobe epilepsy. Epilepsy Res, 2010, 88(1): 23-45.
13.	荣佳, 崔亚洲, 周小艳, 等. 颞叶癫痫大鼠模型海马蛋白质组学的研究. 山东大学学报(医学版), 2011, 49(2): 39-43,53.
14.	孙飞, 周强军, 孙吉, 等. 线粒体呼吸链膜蛋白复合体的结构. 生命科学, 2008, 20(4): 566-578.
15.	陈海浪, 陈喜文, 陈德富. NAD+-依赖型异柠檬酸脱氢酶的结构和功能研究进展. 生物技术通讯, 2003, 14(4): 304-307,311.
16.	Lu J, Holmgren A. The thioredoxin antioxidant system. Free Radic Biol Med, 2014, 66: 75-87.
17.	周润梅. 硫氧还蛋白还原酶的生理学功能研究进展. 现代医药卫生, 2012, 28(16): 2487-2488.
18.	Lee S, Kim SM, LEE RT. Thioredoxin and thioredoxin target proteins: from molecular mechanisms to functional significance. Antioxid Redox Signal, 2013, 18(10): 1165-1207.
19.	Nilanjana Maulik, Dipak K Das. Emerging potential of thioredoxin and thioredoxin interacting proteins in various disease conditions. Biochim Biophys Acta, 2008, 1780(11): 1368-1382.
20.	L. Tao E, Gao NS, Bryan T, et al Cardioprotective effects ofthioredoxin in myocardial ischemia and reperfusion: roleof S-nitrosation [corrected]. Proc Natl Acad Sci USA, 2004, 101: 11471-11476.
21.	魏学文, 张晓伟. 癫痫大鼠海马硫氧还蛋白还原酶活性及cleaved caspase-3表达检测. 检验医学与临床, 2011, 8(22): 2695-2696.

1. Roland D Thijs, Rainer Surges, Terence J O'Brien, et al. Epilepsy in adults. Lancet, 2019, 393(10172): 689-701.
2. 张淑琴, 林卫红, 赵丽曼. 实验性癫痫不同脑区细胞凋亡的观察及意义. 中风与神经疾病杂志, 2002, 19(4): 205-207.
3. Beng zon J, Mohapel P, Ekdahl CT, et al. Neuronal apoptosis after brief and prolonged seizures. Prog Brain Res, 2002, 135: 111-119.
4. Stadtman ER. Protein oxidation in aging and age-related diseases. Ann N. Y. Acad. Sci, 2001, 928: 22-38.
5. Wong-ekkabut J, Xu Z, Triampo W, et al. Effect of lipid peroxidation on the properties of lipid bilayers: a molecular dynamics study. Biophys J, 2007, 93: 4225-4236.
6. Henshall DC, Murphy BM. Modulators of neuronal cell death in epilepsy. Curr Opin Pharmacol, 2008, 8(1): 75-81.
7. 宋修云, 胡金凤, 陈乃宏. 神经细胞凋亡与脑缺血疾病. 中国药理学通报, 2012, 28(3): 307-310. .
8. 王维平, 史志勤, 于江华等. 重组人红细胞生成素对癫痫持续状态大鼠海马p-Akt和caspase-9的影响及作用机制. 南方医科大学学报, 2010, 30(1): 64-69.
9. 史志勤, 王维平, 于江华, 等. 重组人红细胞生成素经PI3K/Akt途径对大鼠癫痫持续状态诱导的神经元凋亡的保护作用. 中国组织化学与细胞化学杂志, 2009, 18(1): 94-102.
10. 张思远, 蔡昕, 卢晓桦, 等. 新型H2S供体激活PI3K/Akt对癫痫大鼠脑内Bcl-2/Bax蛋白表达的影响. 华中科技大学学报(医学版), 2020, 49(3): 296-300.
11. Singh A, Kukreti R, Saso L. Oxidative stress: a key modulator in neurodegenerative disease. Molecules, 2019, 24(8): 1583.
12. Waldbaum S, Patel M. Mitochondrial oxidative stress in temporal lobe epilepsy. Epilepsy Res, 2010, 88(1): 23-45.
13. 荣佳, 崔亚洲, 周小艳, 等. 颞叶癫痫大鼠模型海马蛋白质组学的研究. 山东大学学报(医学版), 2011, 49(2): 39-43,53.
14. 孙飞, 周强军, 孙吉, 等. 线粒体呼吸链膜蛋白复合体的结构. 生命科学, 2008, 20(4): 566-578.
15. 陈海浪, 陈喜文, 陈德富. NAD+-依赖型异柠檬酸脱氢酶的结构和功能研究进展. 生物技术通讯, 2003, 14(4): 304-307,311.
16. Lu J, Holmgren A. The thioredoxin antioxidant system. Free Radic Biol Med, 2014, 66: 75-87.
17. 周润梅. 硫氧还蛋白还原酶的生理学功能研究进展. 现代医药卫生, 2012, 28(16): 2487-2488.
18. Lee S, Kim SM, LEE RT. Thioredoxin and thioredoxin target proteins: from molecular mechanisms to functional significance. Antioxid Redox Signal, 2013, 18(10): 1165-1207.
19. Nilanjana Maulik, Dipak K Das. Emerging potential of thioredoxin and thioredoxin interacting proteins in various disease conditions. Biochim Biophys Acta, 2008, 1780(11): 1368-1382.
20. L. Tao E, Gao NS, Bryan T, et al Cardioprotective effects ofthioredoxin in myocardial ischemia and reperfusion: roleof S-nitrosation [corrected]. Proc Natl Acad Sci USA, 2004, 101: 11471-11476.
21. 魏学文, 张晓伟. 癫痫大鼠海马硫氧还蛋白还原酶活性及cleaved caspase-3表达检测. 检验医学与临床, 2011, 8(22): 2695-2696.

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Study of recombinant human erythropoietin on hippocampal proteomics in pentylenetetrazole-induced epileptic rats

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