YANG Yang 1 , SUN Hongying 2 , Celemuge 1 , Cheliger 1
  • 1. Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014000, China;
  • 2. Department of Neurology, First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014000, China;
SUN Hongying, Email: sunhongying2004@sina.com
Kruppel-like factor 4; Epilepsy; Glutamate; Oxidative stress
10.7507/2096-0247.202411005
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

Objective Explore the mechanism of action of Kruppel-like factor 4 (KLF4) in the oxidative damage model of hippocampal neurons in mice induced by glutamate. Methods To clarify the role of KLF4 and glutamate in the oxidative toxicity of epilepsy, the mouse hippocampal neuron cell line (HT22) was adopted, and a neuronal death excitotoxicity cell model was formed by induction with glutamate as the in vitro epilepsy experimental model. The expression level of KLF4 was detected by Real-Time PCR. HT22 cells were transfected with KLF4-specific siRNA, and the experiments were grouped as follows: Ctrl group, Glu group, Glu + siKLF4-1 group, and Glu + siKLF4-2 group. The cell viability of each group was detected by the CCK8 method. Results KLF4 was significantly increased in the epilepsy model of HT22 cells induced by glutamate, while downregulation of KLF4 improved the proliferation and viability of neurons in the epilepsy model of HT22 cells induced by glutamate. Conclusion In the hippocampal neuron cells of epileptic mice, KLF4 is highly expressed. The downregulation of KLF4 improves the proliferation function and vitality of glutamate-induced HT22 cells, indicating that KLF4 may contribute to the occurrence and development of epilepsy by participating in the regulation of oxidative stress responses.

Citation： YANG Yang, SUN Hongying, Celemuge, Cheliger. The mechanism of Krüppel-like factor 4 in glutamate-induced hippocampal neurons of epileptic mice. Journal of Epilepsy, 2025, 11(1): 41-44. doi: 10.7507/2096-0247.202411005 Copy

Copyright © the editorial department of Journal of Epilepsy of West China Medical Publisher. All rights reserved

  • Previous Article

    Patients with epilepsy who had a first seizure while driving a motor vehicle: a report of five cases WANGYan,  WANG Pinchun,  MA Xinnan,  LU Yeting,  ZHU Xiangyi,  GAO Wei,  XU Min,  LI Yan,  HU Xiaowei

  • Next Article

    Application of graph theory-based brain network in developmental and epileptic encephalopathy SUNXinbin,  LIU Zhisheng,  SUN dan