Expression and Significance of Livin in Human Cholangiocarcinoma Tissues and Cell Line_West China Medical Journal

Authors：

TANG Zhihua ^1,2 , CHEN Jisheng ¹ , YE Lin ² , YU Jiexiong ²

1.Department of Hepatobiliary Surgery，Second Affiliated Hospital of Sun Yatsen University，Guangzhou 510120，2.Base of Postdoctoral Research，Affiliated Jiangmen Central Hospital of Sun Yat-sen University，Jiangmen Guangdong 529030，China;

Corresponding author：

Keywords：

Livin基因; 胆管癌; 基因表达

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目的:探讨Livin基因在人胆管癌组织及胆管癌细胞系中的表达情况及其与胆管癌发生发展之间的关系。方法:采用免疫组织化学技术（SP法）检测Livin基因蛋白在45例人胆管癌标本及及40例癌旁胆管组织、20例正常胆管组织标本中的表达；同时采用RTPCR法及SP 法检测了Livin基因mRNA和蛋白在人胆管癌细胞系QBC939及非肿瘤细胞系HT1080中表达。结果:Livin在胆管癌组织中表达阳性率为57.8%，而癌旁胆管组织、非癌胆管组织中未能检测到Livin表达。Livin表达与性别、年龄、肿瘤大小及肿瘤分化程度无关。在有淋巴结转组中，Livin阳性表达率（70.4%）明显高于无淋巴结转移组（38.9%）。在人胆管癌细胞QBC939 中，Livin mRNA及蛋白均特异性表达，而非肿瘤细胞系HT1080未见Livin表达。结论:Livin 基因在人类胆管癌组织和细胞系中选择性高表达，其可能与胆管癌发生、发展及预后密切相关。

Citation： TANG Zhihua,CHEN Jisheng,YE Lin,YU Jiexiong. Expression and Significance of Livin in Human Cholangiocarcinoma Tissues and Cell Line. West China Medical Journal, 2009, 24(1): 67-69. doi: Copy

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6.	Vucic D，Deshayes K，Ackerly H，et al .SMAC negatively regulates the antiapoptotic activity of melanoma inhibitor of apoptosis （MLIAP）[J].J Biol Chem，2002，277 （14）:12275-12279..
7.	Kim D K，Alvarado C S，Abramowsky C R，et al.Expression of inhibitorofapoptosis protein （IAP） Livin by neuroblastoma cells:correlation with prognostic factors and outcome[J].Pediatr Dev Pathol，2005，8（6）:621-629.

1. Kasof GM，Gomes BC.Livin，a novel inhibitor of apoptosis protein family member[J].J Biol Chem，2001，276（5）:3238-3246..
2. Gazzaniga P，Gradilone A，Giuliani L，et al.Expression and prognostic significance of livin，survivin and other apoptosis related genes in the progression of superficial bladder cancer[J].Ann Oncol，2003，14（1）:85-90..
3. Yagihashi A，Ohmura T，Asanuma K，et al.Detection of autoantibodies to survivin and livin in sera frompatients with breast cancer[J].Clin Chim Acta，2005，362（12）:125-130..
4. Burke EC，Jamagin WR，Hoehwald SN，et al.Hilar cholangiocarcinoma:patterns of spread，the importance of hepatic resection for curative operation，and a presurgical clinical staging system[J].Ann Surg，1998，228:385一394..
5. Isa T，Tomita S，Nakaehi A，et al.Analysis of microsatellite instability，Kras gene mutation and P53 protein over expression intrahepatic cholangiocarcinoma[J]. Hepatogastroenterology，2002，49:604-608..
6. Vucic D，Deshayes K，Ackerly H，et al .SMAC negatively regulates the antiapoptotic activity of melanoma inhibitor of apoptosis （MLIAP）[J].J Biol Chem，2002，277 （14）:12275-12279..
7. Kim D K，Alvarado C S，Abramowsky C R，et al.Expression of inhibitorofapoptosis protein （IAP） Livin by neuroblastoma cells:correlation with prognostic factors and outcome[J].Pediatr Dev Pathol，2005，8（6）:621-629.

West China Medical Journal

Expression and Significance of Livin in Human Cholangiocarcinoma Tissues and Cell Line

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