• 1.Department of Hepatobiliary Surgery,Suining Central Hospital,Suining Sichuan 629000;2.The General Surgery Department,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;
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目的:探讨西南地区雌激素受体α(estrogen receptor α,ERα)基因多态性与原性肝癌关系。方法:选择西南地区100名原发性肝癌患者为实验组,100名非肝病人群作为对照组。应用分子生物学的方法分析PvuⅡ,XbaⅠ限制性片段长度多态性(restriction fragment length polymorphism,RFLP)。同时对人雌激素受体基因上游的短串联重复序列(short tandem repeat,STR)进行纯化、克隆和序列分析,观察ERα基因多态性基因型在实验组与对照组中的基因型分布。结果:PvuⅡ和XbaⅠ限制性片段长度多态性在两组中均呈多态性分布。病例组TA13等位基因频率高于对照组,差异有显著性,TA15等位基因频率低于对照组,差异有显著性。结论:ERα基因多态性与原性肝癌有关,X等位基因可能是其危险因素,P等位基因可能是其保护因素,TA13等位基因可能是其危险因素,TA15等位基因可能是其保护因素。

Citation: ZHANG Wei,SHI De,DU Chengyou,et al.. Study on the Relationship between PvuⅡ,XbaⅠ and STR Polymorphism of the Estrogen Receptor α Gene and Primary Liver Cancer. West China Medical Journal, 2009, 24(1): 70-73. doi: Copy