目的:了解人乳腺癌细胞系MCF-7中的不同亚群细胞的体外增殖特性。方法:采用流式细胞技术从MCF-7细胞中分选出不同的细胞亚群,并测定它们在体外的增殖、克隆能力。结果:和CD44+CD24+细胞相比,MCF-7中的CD44+CD24-细胞具有更强的体外克隆能力,而两者的体外增殖能力相近。结论:MCF7中存在乳腺癌干细胞,而CD44+CD24+细胞可能是乳腺癌祖细胞。
Citation:
LI Zhi,LIU Chunping,HE Yanli,et al. Observation on Culture of Different Cells of MCF-7 in Vitro. West China Medical Journal, 2009, 24(1): 141-143. doi:
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- 1. Suling L,Gabriela D,Max SW.Mammary stem cells,selfrenewal pathways,and carcinogenesis[J].Breast Cancer Res,2005,7:86-95.[9]Tannishtha R,Hans C.Wnt signalling in stem cells and cancer[J].Nature,2005,434:843-850.