目的：研究中药当归芍药散对外周血T淋巴细胞转录因子GATA-3和T-bet mRNA表达的影响，初步探讨其用于治疗不明原因反复自然流产的可能性。方法：体外分离提取11例志愿者外周血单核细胞，在含有或不含当归芍药散的培养液中培养24h，用实时定量PCR技术检测GATA-3和T-bet mRNA的表达。结果：用10 μg/mL浓度当归芍药散处理后,单核细胞中GATA-3 mRNA的含量与对照组比较显著增高（P＜0.05)。当归芍药散处理细胞后，T-bet mRNA的表达水平呈降低趋势，当浓度为100 μg/mL时，与对照组比较有显著性差异（P＜0.05)。结论：当归芍药散可上调转录因子GATA-3 mRNA的表达或下调T-bet mRNA的表达,从而可能通过调节Th2/Th1平衡向Th2偏移、对于Th1反应异常增强的不明原因反复自然流产有一定的治疗潜能。
Citation：
HUANG Zhongying,LI Shangwei,MA Qianhong. Effect of Danggui-shaoyao-san on Expression of GATA-3 and T-bet mRNA in PBMCs. West China Medical Journal, 2008, 23(1): 27-28. doi:
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- 3. 刘春丽.当归芍药散加味治疗先兆流产30例[J].安徽中医学院学报,2000，19(4):33.
- 4. Zhu J,Yamane H,Cote-Sierra J,et al.GATA-3 promotes Th2 responses throughthree different mechanisms:induction of Th2 cytokine production,selective growth of Th2 cells and inhibition of Th1 cell-specific factors[J].Cell Res,2006,16(1):3-10.
- 5. Zheng W-P,Flavell RA.The transcription factor GATA-3 is necessary and sufficient for Th2 cytokinegene expression in CD4 T-cells[J].Cell,1997,89(4):587-596.
- 6. Carp H,Torchinsky A,Tein A,et al.Hormones,cytokines and fetal anomalies in habitual abortion[J].Endocrinol,2001,15(6:)472-483.
- 7. Usuki S,Higa TN,Soreya K.The improvement of luteal insufficiency in fecund women by tokishakuyakusan treatment[J].Am J Chin Med,2002,30(2-3):327-338.
