• 1Department of Pathophysiology,Chengdu Medical College,Cheng du 610083; 2Reproductive Center of Mianyang Central Hospital,Mianyang 621000,China; 3Department of Physiology,West China School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 61004,China; 4Department of Biochemistry,Mdical School of Qinghai University,Xining 810001,China;
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目的:探讨雌激素影响人精子顶体反应的可能机制。方法:应用异硫氰酸荧光素标记豌豆凝集素荧光染色法(FITC-PSA)分析精子顶体反应(AR)、以分光光度比色法测定顶体酶(ACE)活性。结果:17β-雌二醇(17β-E2)可促进精子发生AR,并增强精子顶体酶的活性;去除培养液中的Ca2+后,17β-E2不能诱导精子发生AR;PKC抑制剂能明显降低17β-E2所诱导的AR;E2-BSA亦能够促进精子发生AR,其作用与17β-E2无显著差异。结论:雌激素对人精子顶体反应有一定的促进作用,增强精子顶体酶活性可能是其作用途径之一,此过程涉及了胞外Ca2+、PKC及精子膜上的ER或雌激素结合位点的参与。

Citation: HE Yanfang,ZHENG Jie,YUE Limin,et al.. Effect and Mechanism of 17β-estradiol on Acrosome Reaction in Human Spermatozoa. West China Medical Journal, 2008, 23(1): 34-35. doi: Copy