Effects of Artesunate on Apoptosis of Rat Glomerular Mesangial Cells_West China Medical Journal

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Department of Nephrology,West China Hospital of Sichuan University,Chengdu 610041,China;

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Keywords：

青蒿琥酯; 肾小球系膜细胞; 凋亡

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目的：观察青蒿琥酯对脂多糖诱导的大鼠肾小球系膜细胞(glomerular mesangial cells,GMC)凋亡的影响。方法：用不同浓度的青蒿琥酯刺激脂多糖诱导增殖的大鼠肾小球系膜细胞，HE染色观察凋亡细胞形态，DAPI荧光染色观察细胞核凋亡情况，用流式细胞仪检测细胞凋亡率。结果：青蒿琥酯对系膜细胞凋亡有明显诱导作用，呈一定剂量依赖性。结论：青蒿琥酯能够剂量依赖性地诱导大鼠系膜细胞凋亡。

Citation： MA Xingyi,YANG Min,MI Xuhua,et al.. Effects of Artesunate on Apoptosis of Rat Glomerular Mesangial Cells. West China Medical Journal, 2008, 23(1): 92-93. doi: Copy

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1. Harrison DJ.Cell death in the diseased glomerulus[J].Histopathology，1988，12(6)：679-683..
2. Baker AJ,Mooney A,Hughe-s J,et al.Mesangial cell apoptosis:the major mechanism for resolution of glomerular hypercellularity inexperimental mesangial proliferative nephritis[J].J Clin Invest,1994,94(5):2015-2116..
3. 谢彤，吴平,唐德炎，等.环磷酰胺在体外对大鼠肾小球系膜细胞增殖及凋亡的影响[J].中国病理生理杂志,2003,19(3):391-393..
4. Amore A,Emancipator SN,Cirina P,et al.Nitric oxide mediates cyclosporine-induced apoptosis in cultured renal cell[J].Kidney Int,2000,57(4):1549-1559..
5. 孙田美，梅长林，葛守一，等.洛伐他汀对系膜细胞凋亡及Bcl-2和Bax基因表达的影响[J].中华肾脏病杂志，2003,19(1):38-42..
6. Chen HH,Zhou HJ,Fang X.Inhibition of human cancer cell line growth and human umbilical vein endothelial cell angiogenesis by artemisinin derivatives in vitro[J].Pharmacol Res,2003,48(3):231-236..
7. Posner GH,Northrop J,Paik IH,et al.New chemical and biological aspects of artemisinin－derived trioxanedimers[J].Bioorg Med Chem,2002,10(1)：227-232..
8. Efferth T,Olbrich A,Bauer R，et al.mRNA expression profiles for the response of human tumor cell lines to the antimalarial drugs artesunate,arteether,and artemether[J].Biochem Pharmacol,2002,64(4):617-623..
9. Singh NP,Lai H.Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells[J].Life Sci,2001,70(1)：49-56..
10. 方步武,来丽娜,林雅军,等.青蒿琥酯抗四氯化碳致大鼠肝纤维化的作用[J].中国药理学通报，2005，21（6）：762-763.

West China Medical Journal

Effects of Artesunate on Apoptosis of Rat Glomerular Mesangial Cells

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