Effects of IL-6 on Apoptosis Induced by Hydrogen Peroxide in Alveolar Epithelial Cells_West China Medical Journal

Authors：

1Department of Respiratory Medicine;2Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu 610041,China;

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Keywords：

IL-6; 凋亡; 过氧化氢; caspase-3; caspase-9

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研究白介素6(IL-6)在过氧化氢诱导的肺泡上皮细胞凋亡中的作用及其机制。方法：以人Ⅱ型肺泡上皮细胞A549细胞株为实验对象，MTT法检测IL-6对A549细胞增殖活性的影响，TUNEL检测法检测IL-6对A549细胞凋亡率的影响，Western blot观察caspase-3和caspase-9蛋白量的变化。结果：IL-6能够增加A549细胞的增殖活性，减少其凋亡。与模型组相比，IL-6干预组的caspase-3和caspase-9的蛋白表达量明显降低,有统计学意义。结论：IL-6能够抑制过氧化氢诱导的肺泡上皮细胞凋亡，并且与caspase-3和caspase-9蛋白的表达量下降有关。提示IL-6对肺泡上皮细胞的保护作用可能是通过抑制细胞凋亡相关蛋白来实现的。

Citation： YANG Dongmei,DING Yu,YING Binwu,et al.. Effects of IL-6 on Apoptosis Induced by Hydrogen Peroxide in Alveolar Epithelial Cells. West China Medical Journal, 2008, 23(1): 98-100. doi: Copy

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1. Simpson RJ,Hammacher A,Smith DK,et al.Interleukin-6:structure-function relationships[Ｊ].Protein Sci，1997,6（５）:925-955..
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3. 汪家政，范明.蛋白质技术手册[Ｊ]．第1版.北京：科学出版社，2001：51-54．.
4. Jin XL,Zimmers TA,Perez EA,et al.Paradoxical effects of short-and long-term interleukin-6 exposure on liver injury and repair[J].Hepatology,2006,43(3):474-484..
5. Yamashita T,Sawamoto S，Suzuki S,et al.Blockade of interleukin-6 signaling aggravates ischemic cerebral damage in mice:possible involvement of Stat3 activation in the protection of neurons[J].J Neurochem，2005,94(2):459-468.
6. Hengartner MO.The biochemistry of apoptosis[Ｊ].Nature,2000,407:770-776..

West China Medical Journal

Effects of IL-6 on Apoptosis Induced by Hydrogen Peroxide in Alveolar Epithelial Cells

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