Effect of Decorin on Proliferation of Tenon’s CapsuleFibroblasts Stimulated by TGF-β1_West China Medical Journal

Authors：

PAN Rugang , CHEN Xiaoming , LIU Dongjing , et al.

The Department of Ophthalmology,West China Hospital,Sichuan University,Chengdu 610041,China;

Corresponding author：

Keywords：

转化生长因子β1; 核心蛋白多糖; Tenon囊成纤维细胞

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目的：研究转化生长因子β1（TGF-β1）促进Tenon囊成纤维细胞的增殖作用及核心蛋白多糖（decorin）对TGF-β1促Tenon囊成纤维细胞增殖的影响。方法：（1）取青光眼患者的Tenon囊组织，采用组织块贴壁培养法进行原代培养细胞培养，细胞经过冷冻与复苏后，观察细胞形态结构与生物学特性。（2）用不同浓度(0.01、0.1、1、5、10ng/mL)TGF-β1作用于Tenon囊成纤维细胞48 h。（3）终浓度为5 ng/mL的TGF-β1 和不同浓度(0.01、0.1、1、2、5μg/mL)的decorin共同作用于Tenon囊成纤维细胞48h。MTT比色法检测Tenon囊成纤维细胞增殖。结果：（1）体外成功培养Tenon囊成纤维细胞，细胞生长活跃，形状稳定，细胞经过冷冻与复苏后，仍能保持其原来的细胞形态结构与稳定的生物学特性。（2）1～10ng/mL TGF-β1能促进Tenon囊成纤维细胞的增殖，并与浓度成正比。（3）2～5μg/mL decorin 能抑制TGF-β1促Tenon囊成纤维细胞增殖作用，其抑制作用与浓度成正比。结论： TGF-β1可刺激Tenon囊成纤维细胞增殖，Decorin能抑制TGF-β1促进细胞增殖的作用，其机理可能通过与TGF-β1结合抑制青光眼滤过手术后瘢痕形成。

Citation： PAN Rugang,CHEN Xiaoming,LIU Dongjing,et al.. Effect of Decorin on Proliferation of Tenon’s CapsuleFibroblasts Stimulated by TGF-β1. West China Medical Journal, 2008, 23(1): 101-102. doi: Copy

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1. Brogliatti B,Rigault R,Palanza L,et al.Intraocular pressure and progression of visual field damage[J].Acta Ophthalmol Scand Suppl,2002,236:26-27．.
2. Francis BA,Du LT,Najafi K,et al.Histopathologic features of conjunctival filtering blebs [J].Arch Ophthalmol,2005,123:166-170．.
3. Nomu Y.Structural change in decorin with skin aging[Ｊ].Connect Tissue Res,2006,47(5):249-255．.
4. Nash MA,Loercher AE,Freedman RS.In vitrogrowth inhibition of ovarian cancer cells by decorin:synergism of action between decorin and carboplatin.Cancer Res，1999,59:6192-6196..
5. Choi WH,Ji KA,Jeon SB,et al.Anti-inflammatory roles of retinoic acid in rat brain astrocytes:Suppression of interferon-γ-induced JAK/STATphosphorylation[Ｊ].Biochem Biophys Res Commun,2005,329(1):125-131..
6. Corderio MF.Beyond mitomycin:TGF-β and wound healing[J].Prog Reyin Eye Res,2002,21:75-89．.
7. Tripathi RC,Li J,Chalam KV,et al.Expression of growth factor mRNAs by human Tenon’s capsule fibroblasts[Ｊ].Exp Eye Res,1996,63:339-346.
8. Moustakas A,Souchelnytskyi S,Heldin CH.Smad regulation in TGF-beta singal transduction[Ｊ].J Cell Sci，2001，114(Pt24):4359-4369..
9. Kolb M,Margetts PJ,Sime PJ,et al.Proteoglycans decorin and biglycan differentially modulate TGF-β-mediated fibrotic responses in the lung[Ｊ].Am J Physiol Lung Cell Mol Physiol，2001，280:L1327-Ｌ1334.

West China Medical Journal

Effect of Decorin on Proliferation of Tenon’s CapsuleFibroblasts Stimulated by TGF-β1

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