Growth Characteristics of Pancreatic Cancer Cells in Two-or Three-Dimensional Culture Systems_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

XU Qiang , HU Ya , LIAO Quan ,  ZHAO Yupei.

Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medial Sciences, Beijing 100730, China;

Corresponding author：

ZHAO Yupei., Email: zhao8028@263.net

Keywords：

Pancreatic cancer; Multicellular spheroid; Three-dimensional culture; Type Ⅰ collagen

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ObjectiveTo investigate the growth characteristics of pancreatic cancer cells in the twodimensional culture system (monolayer) and threedimensional culture system (type Ⅰ collagen and extracellular matrix gel). MethodsThree pancreatic cancer cell lines (SW1990, PCT, and ASPC1) were cultured in monolayer, type Ⅰ collagen, and extracellular matrix gel, respectively. The growth patterns were observed, growth curves were detected by CCK8 test, and the cell cycle distributions were analyzed by propidium iodide staining. Results In the twodimensional culture system, cells grew in monolayer. In the type Ⅰ collagen and the ECM gel threedimensional culture system, cells formed multicellular spheroids (MCS), of which the growth rates were slower than those of the cells in monolayer. The proportions of S phase of SW1990, PCT, and ASPC1 cells in twodimensional culture system were significantly more than those in the type Ⅰ collagen on 4 d and 8 d 〔(29.6±3.0)% vs. (18.2±5.1)%, (33.6±2.1)% vs. (14.5±3.2)%, (33.1±1.8)% vs. (24.7±2.6)%; P lt;0.05〕, while the difference of proportion of three cell lines in G2/M phase was not different between twodimensional culture system and type Ⅰ collagen (P gt;0.05). The proportions of G0/G1 phase of SW1990 and PCT cells cultured in the type Ⅰ collagen on 4 d and 8 d and ASPC1 cells cultured in the type Ⅰ collagen on 4 d were significant more than those cultured in twodimensional culture system (P lt;0.05). The proportions of S phase of ASPC1 cells and SW1990 cells cultured in the type Ⅰ collagen on 4 d were significant more than those cultured in the type Ⅰ collagen on 8 d (P lt;0.05). ConclusionsThe characteristics of pancreatic cancer cells in twodimensional and threedimensional culture systems are different. MCS culture system can better mimic the in vivo growth environment of cells in tumors.

Citation： XU Qiang,HU Ya,LIAO Quan,ZHAO Yupei.. Growth Characteristics of Pancreatic Cancer Cells in Two-or Three-Dimensional Culture Systems. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(1): 33-38. doi: Copy

1.	Abbott A. Cell culture: biolog’s new dimension [J]. Nature, 2003, 424(6951): 870872.
2.	Matsuura T. Bioreactors for 3dimensional highdensity culture of human cells [J]. Hum Cell, 2006, 19(1): 1116.
3.	Del Duca D, Werbowetski T, Del Maestro RF. Spheroid preparation from hanging drops: characterization of a model of brain tumor invasion [J]. J Neurooncol, 2004, 67(3): 295303.
4.	Lee GY, Kenny PA, Lee EH, et al. Threedimensional culture models of normal and malignant breast epithelial cells [J]. Nat Methods, 2007, 4(4): 359365.
5.	Lin RZ, Chang HY. Recent advances in threedimensional multicellular spheroid culture for biomedical research [J]. Biotechnol J, 2008, 3(910): 11721184.
6.	周涛, 胡亚, 廖泉, 等. 2种体外肿瘤细胞药敏检测方法的比较研究 [J]. 中国普外基础与临床杂志, 2008, 15(11): 825828.
7.	周涛, 胡亚, 廖泉, 等. 胰腺癌多细胞团簇的化疗药物敏感试验 [J]. 中华胰腺病杂志, 2008, 8(6): 361364.
8.	Mellor HR, Ferguson DJ, Callaghan R. A model of quiescent tumor microregions for evaluating multicellular resistance to chemotherapeutic drugs [J]. Br J Cancer, 2005, 93(3): 302309.
9.	KunzSchughart LA, Freyer JP, Hofstaedter F, et al. The use of 3D cultures for highthroughput screening: the multicellular spheroid model [J]. J Biomol Screen, 2004, 9(4): 273285.
10.	MuellerKlieser W. Tumor biology and experimental therapeutics [J]. Crit Rev Oncol Hematol, 2000, 36(23): 123139.
11.	Friedrich J, Ebner R, KunzSchughart LA. Experimental antitumor therapy in 3D: spheroids-old hat or new challenge? [J]. Int J Radiat Biol, 2007, 83(1112): 849871.
12.	周方正, 伍钢. 细胞伪足与肿瘤转移研究进展 [J]. 临床肿瘤学杂志, 2007, 12(1): 7779.
13.	张秀真, 吴泽志. 细胞伪足形成与微丝骨架研究进展 [J]. 国外医学临床生物化学与检验学分册, 2005, 26(4): 213216.
14.	徐强, 廖泉, 赵玉沛. 多细胞球样体的制备及其在肿瘤治疗研究中的应用 [J]. 基础医学与临床, 2010, 30(3): 320324.
15.	Santini MT, Rainaldi G, Indovina PL. Multicellular tumour spheroids in radiation biology [J]. Int J Radiat Biol, 1999, 75(7): 787799.
16.	Sminia P, Acker H, Eikesdal HP, et al. Oxygenation and response to irradiation of organotypic multicellular spheroids of human glioma [J]. Anticancer Res, 2003, 23(2B): 14611466.
17.	Acker H, Carlsson J, MuellerKlieser W, et al. Comparative pO2 measurements in cell spheroids cultured with different techniques [J]. Br J Cancer, 1987, 56(3): 325327.
18.	DomínguezMuoz JE著, 赵玉沛主译. 临床胰腺病学 [M]. 天津: 天津科技翻译出版公司, 2007: 292293.
19.	潘玉先, 郑振华. 人大隐静脉内皮细胞种植人工血管的实验研究 [J]. 中国普外基础与临床杂志, 2000, 7(1): 1315.
20.	施森, 何延政, 刘勇, 等. 外周血来源内皮祖细胞三维血管新生模型的建立及其特性分析 [J]. 中国普外基础与临床杂志, 2009, 16(2): 119123.
21.	Gen M, Castro Kreder N, Bartenvan Rijbroek A, et al. Enhancement of effects of irradiation by gemcitabine in a glioblastoma cell line and cell line spheroids [J]. J Cancer Res Clin Oncol, 2004, 130(1): 4551.
22.	Muir CP, Adams MA, Graham CH. Nitric oxide attenuates resistance to doxorubicin in threedimensional aggregates of human breast carcinoma cells [J]. Breast Cancer Res Treat, 2006, 96(2): 169176.
23.	Chua YJ, Zalcberg JR. Pancreatic cancer-is the wall crumbling? [J]. Ann Oncol, 2008, 19(7): 12241230.
24.	Vulfovich M, RochaLima C. Novel advances in pancreatic cancer treatment [J]. Expert Rev Anticancer Ther, 2008, 8(6): 9931002.
25.	Boeck S, Heinemann V. The role of secondline chemotherapy after gemcitabine failure in patients with advanced pancreatic cancer [J]. Future Oncol, 2008, 4(1): 4150.
26.	Tempero M, Plunkett W, Ruiz Van Haperen V, et al. Randomized Phase Ⅱ comparison of doseintense gemcitabine: thirtyminute infusion and fixed dose rate infusion in patients with pancreatic adenocarcinoma [J]. J Clin Oncol, 2003, 21(18): 34023408.
27.	Louvet C, Labianca R, Hammel P, et al. Gemcitabine in combination with oxaliplatin compared with gemcitabine alone in locally advanced or metastatic pancreatic cancer: results of a GERCOR and GISCAD phase Ⅲ trial [J]. J Clin Oncol, 2005, 23(15): 35093516.

1. Abbott A. Cell culture: biolog’s new dimension [J]. Nature, 2003, 424(6951): 870872.
2. Matsuura T. Bioreactors for 3dimensional highdensity culture of human cells [J]. Hum Cell, 2006, 19(1): 1116.
3. Del Duca D, Werbowetski T, Del Maestro RF. Spheroid preparation from hanging drops: characterization of a model of brain tumor invasion [J]. J Neurooncol, 2004, 67(3): 295303.
4. Lee GY, Kenny PA, Lee EH, et al. Threedimensional culture models of normal and malignant breast epithelial cells [J]. Nat Methods, 2007, 4(4): 359365.
5. Lin RZ, Chang HY. Recent advances in threedimensional multicellular spheroid culture for biomedical research [J]. Biotechnol J, 2008, 3(910): 11721184.
6. 周涛, 胡亚, 廖泉, 等. 2种体外肿瘤细胞药敏检测方法的比较研究 [J]. 中国普外基础与临床杂志, 2008, 15(11): 825828.
7. 周涛, 胡亚, 廖泉, 等. 胰腺癌多细胞团簇的化疗药物敏感试验 [J]. 中华胰腺病杂志, 2008, 8(6): 361364.
8. Mellor HR, Ferguson DJ, Callaghan R. A model of quiescent tumor microregions for evaluating multicellular resistance to chemotherapeutic drugs [J]. Br J Cancer, 2005, 93(3): 302309.
9. KunzSchughart LA, Freyer JP, Hofstaedter F, et al. The use of 3D cultures for highthroughput screening: the multicellular spheroid model [J]. J Biomol Screen, 2004, 9(4): 273285.
10. MuellerKlieser W. Tumor biology and experimental therapeutics [J]. Crit Rev Oncol Hematol, 2000, 36(23): 123139.
11. Friedrich J, Ebner R, KunzSchughart LA. Experimental antitumor therapy in 3D: spheroids-old hat or new challenge? [J]. Int J Radiat Biol, 2007, 83(1112): 849871.
12. 周方正, 伍钢. 细胞伪足与肿瘤转移研究进展 [J]. 临床肿瘤学杂志, 2007, 12(1): 7779.
13. 张秀真, 吴泽志. 细胞伪足形成与微丝骨架研究进展 [J]. 国外医学临床生物化学与检验学分册, 2005, 26(4): 213216.
14. 徐强, 廖泉, 赵玉沛. 多细胞球样体的制备及其在肿瘤治疗研究中的应用 [J]. 基础医学与临床, 2010, 30(3): 320324.
15. Santini MT, Rainaldi G, Indovina PL. Multicellular tumour spheroids in radiation biology [J]. Int J Radiat Biol, 1999, 75(7): 787799.
16. Sminia P, Acker H, Eikesdal HP, et al. Oxygenation and response to irradiation of organotypic multicellular spheroids of human glioma [J]. Anticancer Res, 2003, 23(2B): 14611466.
17. Acker H, Carlsson J, MuellerKlieser W, et al. Comparative pO2 measurements in cell spheroids cultured with different techniques [J]. Br J Cancer, 1987, 56(3): 325327.
18. DomínguezMuoz JE著, 赵玉沛主译. 临床胰腺病学 [M]. 天津: 天津科技翻译出版公司, 2007: 292293.
19. 潘玉先, 郑振华. 人大隐静脉内皮细胞种植人工血管的实验研究 [J]. 中国普外基础与临床杂志, 2000, 7(1): 1315.
20. 施森, 何延政, 刘勇, 等. 外周血来源内皮祖细胞三维血管新生模型的建立及其特性分析 [J]. 中国普外基础与临床杂志, 2009, 16(2): 119123.
21. Gen M, Castro Kreder N, Bartenvan Rijbroek A, et al. Enhancement of effects of irradiation by gemcitabine in a glioblastoma cell line and cell line spheroids [J]. J Cancer Res Clin Oncol, 2004, 130(1): 4551.
22. Muir CP, Adams MA, Graham CH. Nitric oxide attenuates resistance to doxorubicin in threedimensional aggregates of human breast carcinoma cells [J]. Breast Cancer Res Treat, 2006, 96(2): 169176.
23. Chua YJ, Zalcberg JR. Pancreatic cancer-is the wall crumbling? [J]. Ann Oncol, 2008, 19(7): 12241230.
24. Vulfovich M, RochaLima C. Novel advances in pancreatic cancer treatment [J]. Expert Rev Anticancer Ther, 2008, 8(6): 9931002.
25. Boeck S, Heinemann V. The role of secondline chemotherapy after gemcitabine failure in patients with advanced pancreatic cancer [J]. Future Oncol, 2008, 4(1): 4150.
26. Tempero M, Plunkett W, Ruiz Van Haperen V, et al. Randomized Phase Ⅱ comparison of doseintense gemcitabine: thirtyminute infusion and fixed dose rate infusion in patients with pancreatic adenocarcinoma [J]. J Clin Oncol, 2003, 21(18): 34023408.
27. Louvet C, Labianca R, Hammel P, et al. Gemcitabine in combination with oxaliplatin compared with gemcitabine alone in locally advanced or metastatic pancreatic cancer: results of a GERCOR and GISCAD phase Ⅲ trial [J]. J Clin Oncol, 2005, 23(15): 35093516.

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Growth Characteristics of Pancreatic Cancer Cells in Two-or Three-Dimensional Culture Systems

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