Animal Experiment Study for The Efficacy of Xiaochengqi-Mixture on Promoting Healing of Colonic Stoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SU Dan ,  LAN Ping , REN Donglin , CHEN Jingbao , LIN Hongcheng.

中山大学附属第六医院结直肠肛门外科（广东广州 510655）;

Corresponding author：

LAN Ping, Email: imysms2222002@yahoo.com.cn

Keywords：

小承气合剂; 吻合口漏; 组织愈合; 羟脯氨酸; Masson染色; 胶原纤维

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo evaluate the efficacy of XiaochengqiMixture (XM) on promoting healing of colonic stoma. MethodsForty Wistar rats were divided into two groups randomly after colonectomy: experimental group (n=20) and control group (n=20). In early postoperatively stage rats were given gastric administration of XM in the experimental group and pure water in the control group. On day 3, 7, and 14 after establishment of animal models, laparotomy was performed in two groups of rats, respectively. Anastomotic stoma and surrounding tissues were harvested to detect the context of hydroxyproline and collagen fiber proportion by Masson dying. ResultsOn day 3 after establishment of animal models, hyperplastic collagen with small fiber was observed while no fasciculus was found. Hydroxyproline context and collagen fiber proportion of rats were higher in experimental group than those in control group (P lt;0.05). On day 7 after operation, many fasciculuses were found in two groups of rats, hydroxyproline context and collagen fiber proportion of rats were higher in experimental group than those in control group (P lt;0.01). On day 14 after operation, fasciculuses became bigger and more regular in arrangement, but there was no significant difference between the two groups (P gt;0.05). ConclusionXM is capable of promoting healing of colonic stoma and might prevent the occurrence of anastomotic fistula.

Citation： SU Dan,LAN Ping,REN Donglin,CHEN Jingbao,LIN Hongcheng.. Animal Experiment Study for The Efficacy of Xiaochengqi-Mixture on Promoting Healing of Colonic Stoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(1): 39-42. doi: Copy

1.	苏丹, 任东林, 林宏城, 等. 术后早期灌服小承气合剂对大鼠结肠吻合术后吻合口愈合的影响 [J]. 广州中医药大学学报, 2010, 27(2): 133136, 201203.
2.	陈经宝, 任东林, 蔡北源, 等. 小承气合剂早期灌服对结肠吻合术后胃肠动力恢复及安全性的作用 [J]. 广东医学, 2007, 28(3): 473475.
3.	李康, 朱佐江. 机能实验学 [M]. 北京: 人民军医出版社, 2000: 6465.
4.	涂小煌，张再重，宋京翔, 等. 大口径肛管预防直肠癌术后吻合口漏的对比研究 [J]. 中国普外基础与临床杂志, 2009, 16(10): 830833.
5.	黄从云，张小龙，朱剑华, 等. 小肠浆肌层吻合与二层吻合的临床随机对照研究 [J]. 中国普外基础与临床杂志, 2009, 16(5): 397399.
6.	李幼生, 鲍扬, 江涛, 等. 生长激素、纤维蛋白胶促进腹腔感染大鼠肠吻合口愈合的动态研究 [J]. 肠外与肠内营养, 2004, 11(3): 147150.
7.	鲍扬, 李幼生, 黎介寿. 早期肠内营养联合生长激素及生物蛋白胶对腹腔感染大鼠肠吻合口愈合的影响 [J]. 肠外与肠内营养, 2004, 11(5): 270273.
8.	Dinc S, Gulcelik MA, Kuru B, et al. Effects of locally applied recombinant human granulocytemacrophage colonystimulating factor on ischemic bowel anastomoses in rat [J]. Eur Surg Res, 2004, 36(1): 5963.
9.	Dinc S, Alagol H, Gulcelik MA, et al. Locally applied granulocytemacrophage colonystimulating factor improves the impaired bowel anastomoses in rats with longterm corticosteroid treatment [J]. World J Surg, 2002, 26(10): 12081213.
10.	潘立群, 陈荣明, 郭强. 丹参对家兔小肠吻合口肉芽组织修复作用的观察 [J]. 江苏中医, 2000, 21(1): 3839.
11.	金惠铭. 丹参制剂的临床应用及其活血化瘀原理的研究 [J]. 中华医学杂志, 1978, 58(3): 180182.

1. 苏丹, 任东林, 林宏城, 等. 术后早期灌服小承气合剂对大鼠结肠吻合术后吻合口愈合的影响 [J]. 广州中医药大学学报, 2010, 27(2): 133136, 201203.
2. 陈经宝, 任东林, 蔡北源, 等. 小承气合剂早期灌服对结肠吻合术后胃肠动力恢复及安全性的作用 [J]. 广东医学, 2007, 28(3): 473475.
3. 李康, 朱佐江. 机能实验学 [M]. 北京: 人民军医出版社, 2000: 6465.
4. 涂小煌，张再重，宋京翔, 等. 大口径肛管预防直肠癌术后吻合口漏的对比研究 [J]. 中国普外基础与临床杂志, 2009, 16(10): 830833.
5. 黄从云，张小龙，朱剑华, 等. 小肠浆肌层吻合与二层吻合的临床随机对照研究 [J]. 中国普外基础与临床杂志, 2009, 16(5): 397399.
6. 李幼生, 鲍扬, 江涛, 等. 生长激素、纤维蛋白胶促进腹腔感染大鼠肠吻合口愈合的动态研究 [J]. 肠外与肠内营养, 2004, 11(3): 147150.
7. 鲍扬, 李幼生, 黎介寿. 早期肠内营养联合生长激素及生物蛋白胶对腹腔感染大鼠肠吻合口愈合的影响 [J]. 肠外与肠内营养, 2004, 11(5): 270273.
8. Dinc S, Gulcelik MA, Kuru B, et al. Effects of locally applied recombinant human granulocytemacrophage colonystimulating factor on ischemic bowel anastomoses in rat [J]. Eur Surg Res, 2004, 36(1): 5963.
9. Dinc S, Alagol H, Gulcelik MA, et al. Locally applied granulocytemacrophage colonystimulating factor improves the impaired bowel anastomoses in rats with longterm corticosteroid treatment [J]. World J Surg, 2002, 26(10): 12081213.
10. 潘立群, 陈荣明, 郭强. 丹参对家兔小肠吻合口肉芽组织修复作用的观察 [J]. 江苏中医, 2000, 21(1): 3839.
11. 金惠铭. 丹参制剂的临床应用及其活血化瘀原理的研究 [J]. 中华医学杂志, 1978, 58(3): 180182.

Previous Article
重症肌无力危象长期持续状态成功脱离呼吸机一例

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Animal Experiment Study for The Efficacy of Xiaochengqi-Mixture on Promoting Healing of Colonic Stoma

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Format

Content