Discussion and Experiences in Establishing Rat Models of Cavernous Transformation of Portal Vein_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Jianyao ¹ ,  LIU Lei ² , WANG Bin ² , CHEN Fang ² , MAO Jianxiong ² , YAO Jun ³ , CAO Juan ²

1.Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China;;
2.Department of General Surgery, Shenzhen Children’s Hospital, Shenzhen 518026, Guangdong Province, China;;
3.The Second Affiliated Hospital of Jinan University, Shenzhen 518026, Guangdong Province, China;

Corresponding author：

LIU Lei, Email: liulei3322@yahoo.com.cn

Keywords：

Cavernous transformation of portal vein; Sprague-Dawley rat; Animal model

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To explore adaptive condition of preparation of animal model and afford reliable and stable model animal for further research on clinical diagnosis and treatment of cavernous transformation of portal vein (CTPV) by establishment of animal model by partial portal vein stenosis.
Methods According to different straight blunttip needles used, 80 healthy Sprague-Dawley rats were randomly averagely divided into 4 groups: sham operation group, gauge 19 (19G) group, gauge 21 (21G) group and gauge 23 (23G) group. Six weeks after model making, pressure measurement and angiography of portal vein and pathological examination of portal vein and its surrounding tissues were used to evaluate portal hypertension and CTPV.
Results Six weeks after model making no rat died in sham operation group, while the numbers of died rats in 19G group, 21G group and 23G group were 2, 4 and 16, respectively. No portal hypertension was displayed in sham operation group and 19G group 6 weeks after model making. Portography showed that the portal vein seemed smooth without variceal and dilatation in sham operation group and 19G group. Pathological examination demonstrated that the portal vein walls were not enlarged, endothelial cells were smooth. The smooth muscles of middle membrane were not thickened and adventitia was intact. Portal pressure increased and CTPV formed in 21G group and 23G group 6 weeks after model making. Portography showed that collateral circulation formed around portal vein in 21G group and 23G group. The vessel lumens with different size and irregular shapes were displayed by pathological examination. Within the narrow fibrous septum between there were the lumens the fat cells, scattered lymphocytes and mast cells, etc. The portal vein walls were enlarged notably, endothelial cells were damaged, the smooth muscle of middle membrane were thickened, thrombosis were formed.
Conclusion Establishment of CTPV animal models by partial portal vein stenosis is a reliable method. 21G blund-tip needles fits well in the preparation of CTPV, which is reliable and stable with lower mortality.

Citation： WANG Jianyao ,LIU Lei,WANG Bin,CHEN Fang,MAO Jianxiong,YAO Jun,CAO Juan. Discussion and Experiences in Establishing Rat Models of Cavernous Transformation of Portal Vein. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(1): 46-51. doi: Copy

1.	王欣博, 童中勋, 刘威, 等. 门静脉海绵样变性外科治疗的研究进展 [Ｊ]. 中国普外基础与临床杂志, 2008; 15(12): 877879.
2.	Omokawa S, Arai Y, Saito H, et al. A simple experimental model of total hepatectomy, hepatic ischemia and extrahepatic portal obstruction in rats using splenic transposition [Ｊ]. Jpn J Surg, 1991; 21(1): 5056.
3.	Aller MA, Vara E, García C, et al. Prehepatic portal hypertension worsens the enterohepatic redox balance in thioacetamidecirrhotic rats [Ｊ]. Pathophysiology, 2008; 15(4): 233242.
4.	段进东, 管洪庚, 陈易人, 等. 一期门静脉缩窄法复制大鼠门静脉高压性胃病模型 [Ｊ]. 中华实验外科杂志, 2000; 17(4): 356357.
5.	周岩冰, 冷希圣, 齐桂英, 等. 肝前型门静脉高压大鼠门体分流及循环动力学的实验研究 [Ｊ]. 中华外科杂志, 1997; 35(4): 244247.
6.	马秀现, 李晶义. 干细胞移植联合血管内皮生长因子对门静脉高压症大鼠腹膜后交通支形成的影响 [Ｊ]. 中国组织工程研究与临床康复, 2008; 12(38): 74517454.
7.	SánchezPatán F, Anchuelo R, Aller MA, et al. Chronic prehepatic portal hypertension in the rat: is it a type of metabolic inflammatory syndrome? [Ｊ]. Lipids Health Dis, 2008; 13; 7: 4.
8.	Ishikawa T, Sarfeh IJ, Tarnawski A, et al. Epidermal growth factor protects portal hypertensive gastric mucosa in ischemia/reperfusion: the role of capillary endothelia and prostaglandins [Ｊ]. Surgery, 1992, 112(2): 341346.
9.	赵莉, 李振东, 于增文, 等. 肝前型门静脉高压症动物(犬)模型制作体会 [Ｊ]. 中华小儿外科杂志, 2001; 22(1): 4244.
10.	郭美祥, 高丽华, 刘力波, 等. 大鼠门静脉分支结扎后肝再生的实验研究 [Ｊ]. 中国普外基础与临床杂志, 2006; 13(5): 550554.
11.	徐辉, 陈虹彬, 蒋明德, 等. 合金支架治疗食管静脉曲张出血的实验研究 [Ｊ]. 西南国防医药, 2003; 13(4): 358360.
12.	Zhuang ZW, Hoopes PJ, Koutras PC. Transjugular intrahepatic portosystemic shunt with an autologous veincovered stent： results in a swine model [Ｊ]. J Vascular Interv Radiol, 2001; 12(11): 13331342.
13.	王继见, 高根五, 刘长安, 等. 用Wistar大鼠门静脉部分结扎制作门脉高压动物模型 [Ｊ].重庆医科大学学报, 2001; 26(3): 302304.
14.	Soma J, Saito T, Sato H, et al. Membranoproliferative glomerulonephritis induced by portosystemic shunt surgery for noncirrhotic portal hypertension [Ｊ]. Clin Nephrol, 1997; 48(5): 274281.
15.	Bayraktar Y, Tuncer ZS, Kabuku A, et al. Pregnancy complicated by congenital hepatic fibrosis with cavernous transformation of the portal vein: a case report [Ｊ]. Am J Obstet Gynecol, 1997; 177(2): 459461.

1. 王欣博, 童中勋, 刘威, 等. 门静脉海绵样变性外科治疗的研究进展 [Ｊ]. 中国普外基础与临床杂志, 2008; 15(12): 877879.
2. Omokawa S, Arai Y, Saito H, et al. A simple experimental model of total hepatectomy, hepatic ischemia and extrahepatic portal obstruction in rats using splenic transposition [Ｊ]. Jpn J Surg, 1991; 21(1): 5056.
3. Aller MA, Vara E, García C, et al. Prehepatic portal hypertension worsens the enterohepatic redox balance in thioacetamidecirrhotic rats [Ｊ]. Pathophysiology, 2008; 15(4): 233242.
4. 段进东, 管洪庚, 陈易人, 等. 一期门静脉缩窄法复制大鼠门静脉高压性胃病模型 [Ｊ]. 中华实验外科杂志, 2000; 17(4): 356357.
5. 周岩冰, 冷希圣, 齐桂英, 等. 肝前型门静脉高压大鼠门体分流及循环动力学的实验研究 [Ｊ]. 中华外科杂志, 1997; 35(4): 244247.
6. 马秀现, 李晶义. 干细胞移植联合血管内皮生长因子对门静脉高压症大鼠腹膜后交通支形成的影响 [Ｊ]. 中国组织工程研究与临床康复, 2008; 12(38): 74517454.
7. SánchezPatán F, Anchuelo R, Aller MA, et al. Chronic prehepatic portal hypertension in the rat: is it a type of metabolic inflammatory syndrome? [Ｊ]. Lipids Health Dis, 2008; 13; 7: 4.
8. Ishikawa T, Sarfeh IJ, Tarnawski A, et al. Epidermal growth factor protects portal hypertensive gastric mucosa in ischemia/reperfusion: the role of capillary endothelia and prostaglandins [Ｊ]. Surgery, 1992, 112(2): 341346.
9. 赵莉, 李振东, 于增文, 等. 肝前型门静脉高压症动物(犬)模型制作体会 [Ｊ]. 中华小儿外科杂志, 2001; 22(1): 4244.
10. 郭美祥, 高丽华, 刘力波, 等. 大鼠门静脉分支结扎后肝再生的实验研究 [Ｊ]. 中国普外基础与临床杂志, 2006; 13(5): 550554.
11. 徐辉, 陈虹彬, 蒋明德, 等. 合金支架治疗食管静脉曲张出血的实验研究 [Ｊ]. 西南国防医药, 2003; 13(4): 358360.
12. Zhuang ZW, Hoopes PJ, Koutras PC. Transjugular intrahepatic portosystemic shunt with an autologous veincovered stent： results in a swine model [Ｊ]. J Vascular Interv Radiol, 2001; 12(11): 13331342.
13. 王继见, 高根五, 刘长安, 等. 用Wistar大鼠门静脉部分结扎制作门脉高压动物模型 [Ｊ].重庆医科大学学报, 2001; 26(3): 302304.
14. Soma J, Saito T, Sato H, et al. Membranoproliferative glomerulonephritis induced by portosystemic shunt surgery for noncirrhotic portal hypertension [Ｊ]. Clin Nephrol, 1997; 48(5): 274281.
15. Bayraktar Y, Tuncer ZS, Kabuku A, et al. Pregnancy complicated by congenital hepatic fibrosis with cavernous transformation of the portal vein: a case report [Ｊ]. Am J Obstet Gynecol, 1997; 177(2): 459461.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Discussion and Experiences in Establishing Rat Models of Cavernous Transformation of Portal Vein

Abstract Full text Figures/Tables Video References Cited by

Format

Content