Clinical Significance of Screening Serum Protein Fingerprint in Gastric Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Chun ¹ , XU Liang ² , HAN Haibin ³ , YANG Jianbi ¹ , LUO Bing ¹ , PENG Chao. ¹

1.Department of General Surgery, The Second Peoples Hospital of Neijiang of Sichuan Province， Neijiang 641000, China;;
2.Department of Gastrointestinal Surgery, The Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China;;
3.Department of General Surgery, Chang’an Hospital of Chongqing City, Chongqing 400023, ChinaCorresponding Author: XU Liang, E-mail: XuL1104@163.com;

Corresponding author：

Keywords：

Gastric cancer; Proteomic; Serum marker; Surface-enhanced laser desorption-ionization time-of-flight mass spectrometry; Diagnosis

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Objective To detect the serum protein fingerprint in gastric cancer patients by using the surface-enhanced laser desorption-ionization time-of-flight mass spectrometry (SELDI-TOF-MS) and protein chip array technology, screen biomarker candites, build diagnostic models and evaluate its clinical significance. Methods The serum proteomic patterns were detected in 40 patients with gastric cancer, 20 patients with gastric ulcer and 20 healthy blood donors. The diagnostic models were developed and valited by discriminant analysis. Results The peak intensity of differential expression proteins was not found in healthy blood donors, and 1 case was found in patient with gastric ulcer (m/z: 5 910,4 095). The peak intensity of 5 329, 4 095, 5 910, 8 691 and 3 300 (m/z) proteins were significantly higher in 40 gastric cancer patients than those in 20 gastric ulcer patients and 20 healthy blood donors ( P ＜0.05）. Three differential expression proteins were set up a diagnostic model together to diagnose gastric cancer. The diagnostic model made up of the differential expression proteins of 4 095, 5 910 and 8 691 had a sensitivity of 92.5% and a specificity of 97.5% . Conclusion Using SELDI-TOF-MS shows great potential to detect, and screen novel and better biomarkers for gastric cancer.

Citation： LIU Chun,XU Liang,HAN Haibin,YANG Jianbi,LUO Bing,PENG Chao.. Clinical Significance of Screening Serum Protein Fingerprint in Gastric Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(1): 23-27. doi: Copy

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19.	肖雪媛，卫秀平，何大澄. 应用蛋白质芯片技术从血清中筛选肺癌标志蛋白 [Ｊ] . 中国科学C辑， 2003； 33(4)：323.

1. 詹友庆，李威，孙晓卫，等. 胃癌外科治疗的远期疗效研究 [Ｊ] . 中华外科杂志， 2005； 43（17）： 1109.
2. ３ Adam BL, Qu Y, Davis JW, et al . Serum protein fingerprinting coupled with a pattern-matching algorithm distinguishes prostate cancer from benign prostate hyperplasia and healthy men [Ｊ] . Cancer Res, 2002; 62(13)： 3609.
3. ４张建中，郑燕华，冯凯，等. SELDI蛋白指纹技术在肿瘤早期诊断中的应用 [Ｊ] . 世界华人消化杂志， 2004； 12(12)： 2773.
4. ５陈军周，周宁新，刘博，等. 应用SELDI-TOF-MS技术分析胆管恶性肿瘤患者外周血清蛋白的差异表达 [Ｊ] . 中国普外基础与临床杂志， 2008； 15(2)： 88.
5. ６高春芳，赵光，宋国英，等．用飞行质谱技术仪筛选结直肠癌患者中特异性生物标记物的临床意义 [Ｊ] . 中华检验医学杂志， 2003； 26(11)： 658.
6. ７徐文鸿，陈益定，胡跃，等. 激光解吸电离-飞行时间质谱技术和CM10蛋白质芯片检测术前大肠癌分期的意义 [Ｊ] . 中华肿瘤杂志， 2006; 28(10)： 753.
7. ８徐顺福，张国新，施瑞华，等. 胃溃疡内镜随访价值分析 [Ｊ] . 胃肠病学和肝病学杂志， 2007； 16(5)： 444.
8. ９ Farinati F, Cardin F, Di Mario F, et al . Early and advanced gastric cancer during follow-up of apparently benign gastric ulcer： significance of the presence of epithelial dysplasia [Ｊ] . J Surg Oncol, 1987; 36(4)：263.
9. 张雪峰，蒋会勇. 胃癌与肿瘤标记物 [Ｊ] . 中国普外基础与临床杂志， 2002； 9(3)： 143.
10. Suzuki K, Kashimura H, Ohkawa J, et al . Expression of human telomerase catalytic subunit gene in cancerous and precancerous gastric conditions [Ｊ] . J Gastroenterol Hepatol, 2000; 15(7)： 744.
11. van Wijk KJ. Challenges and prospects of plant proteomics [Ｊ] . Plant Physiol, 2001; 126(2)： 501.
12. 金锋，王舒宝，徐惠绵，等．血清CEA术前术后水平与胃癌生物学行为及预后关系的研究 [Ｊ] ．中国肿瘤临床， 1999； 26(8)： 596.
13. 王海燕，姚桂芹，高佩增，等. CA242， CEA， CA199在胃癌及癌前病变组织中表达的相关研究 [Ｊ] . 临床消化病杂志， 2005； 17(6)： 279.
14. Weinberger SR, Dalmasso EA, Fung ET. Current achievements using ProteinChip Array technology [Ｊ] . Curr Opin Chem Biol, 2002; 6(1)： 86.
15. Wu W, Hu W, Kavanagh JJ. Proteomics in cancer research [Ｊ] . Int J Gynecol Cancer, 2002; 12(5)： 409.
16. 郑顺贞，邹声泉. 比较蛋白质组学在肿瘤标志物研究中的应用 [Ｊ] . 中国普外基础与临床杂志， 2008； 15(2)： 147.
17. Grizzle WE, Semmes OJ, Basler J, et al . The early detection research network surface-enhanced laser desorption and ionization prostate cancer detection study： A study in biomarker validation in genitourinary oncology [Ｊ] . Urol Oncol, 2004; 22(4)： 337.
18. Zhu XD, Zhang WH, Li CL, et al . New serum biomarkers for detection of HBV-induced liver cirrhosis using SELDI protein chip technology [Ｊ] . World J Gastroenterol， 2004; 10(16)：2327.
19. 肖雪媛，卫秀平，何大澄. 应用蛋白质芯片技术从血清中筛选肺癌标志蛋白 [Ｊ] . 中国科学C辑， 2003； 33(4)：323.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Clinical Significance of Screening Serum Protein Fingerprint in Gastric Cancer

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