Expressions and Significance of Nerve Growth Factor and Its High Affinity Receptor in Connective Tissue of Bile Duct_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Lei , DENG Xiaoqiang , SHU Yi , ZHENG Shunzhen , ZOU Shengquan

*Department of Biliary and Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaCorresponding Author: ZOU Sheng-quan, E-mail: sqzou@tjh.tjmu.edu.cn;

Corresponding author：

Keywords：

Biliary obstruction; Immunohistochemistry; Nerve growth factor; Jaundice; Connective tissue

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the effects and significance of nerve growth factor (NGF) and its high affinity receptor of tyrosine kinase A (TrkA) expressions on proliferative connective tissue of bile duct in rats after bile duct ligation (BDL). Methods Forty-six female Sprague-Dawley rats were randomly divided into two groups: control group ( n =6) and BDL group ( n =40). The model of obstructive jaundice in rat was made by bile duct ligation, then duodenohepatic ligament was taken and treated with anti-NGF and anti-TrkA receptor antibody. Expressions of NGF and TrkA receptor in connective tissue of bile duct were investigated by immunohistochemistry, blood specimens were collected from left ventricle to detect serum total bilirubin (TB) and alanine aminotransferase (ALT). Results After BDL, TB level obviously elevated in the third day, and continued until the fourteenth day, then descended. By day 21 and 28, it returned to normal level. Compared with normal bile duct, due to bile stasis, an increased thickness of the bile duct wall was observed by microscope which correlated with the proliferation and differentiation of connective tissue cell. NGF and TrkA were expressed by the cell membrane and the cytoplasm of connective tissue cell and inflammatory infiltration cell after BDL. The trend between their expressions and bilirubin levels was similar. Conclusion NGF and its receptor TrkA regulate the proliferate and differentiation of connective cell in bile duct. They may play a key role in the formation of bile duct scar, which seems to be hardly reversed by relief of bile stasis in a short time.

Citation： WANG Lei,DENG Xiaoqiang,SHU Yi,ZHENG Shunzhen,ZOU Shengquan,.. Expressions and Significance of Nerve Growth Factor and Its High Affinity Receptor in Connective Tissue of Bile Duct. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(1): 39-43. doi: Copy

1.	Guyot C, Combe C, Desmoulière A. The common bile duct ligation in rat: A relevant in vivo model to study the role of mechanical stress on cell and matrix behaviour [Ｊ] . Histochem Cell Biol, 2006; 126(4)：517.
2.	Gigliozzi A, Alpini G, Baroni GS, et al . Nerve growth factor modulates the proliferative capacity of the intrahepatic biliary epithelium in experimental cholestasis [Ｊ] . Gastroenterology, 2004; 127(4)：1198.
3.	Aloe L, Bracci-Laudiero L, Bonini S, et al . The expanding role of nerve growth factor: from neurotrophic activity to immunologic diseases [Ｊ] . Allergy, 1997; 52(9)：883.
4.	Freund-Michel V, Frossard N. The nerve growth factor and its receptors in airway inflammatory diseases [Ｊ] . Pharmacol Ther, 2008; 117(1)：52.
5.	Alvaro D, Mancino MG, Onori P, et al . Estrogens and the pathophysiology of the biliary tree [Ｊ] . World J Gastroenterol, 2006; 12(22)：3537.
6.	Takahashi H, Uno S, Watanabe Y, et al . Expression of nerve growth factor-induced type 1 plasminogen activator inhibitor (PAI-1) mRNA is inhibited by genistein and wortmannin [Ｊ] . Neuroreport, 2000; 11(5)：1111.
7.	Olgart Hglund C, de Blay F, Oster JP, et al . Nerve growth factor levels and localisation in human asthmatic bronchi [Ｊ] . Eur Respir J, 2002; 20(5)：1110.
8.	Freund-Michel V, Bertrand C, Frossard N. TrkA signalling pathways in human airway smooth muscle cell proliferation [Ｊ] . Cell Signal, 2006; 18(5)：621.
9.	Levi-Montalcini R. The nerve growth factor 35 years later [Ｊ] . Science, 1987; 237(4819)：1154.
10.	尹兰宁，侯治军，宋爱琳，等. 良性梗阻性黄疸患者血清中IGF-1变化的意义 [Ｊ] . 中国普外基础与临床杂志， 2005; 12(4)：349.
11.	刘双海，蔡景修，顾红光，等. 炎性狭窄胆管壁中成纤维细胞表型改变及增殖的意义 [Ｊ] . 中国普外基础与临床杂志， 2000; 7(2)：69.
12.	Kountouras J, Scheuer PJ, Billing BH. Effect of prolonged bile duct obstruction in the rat on hepatic transport of bilirubin [Ｊ] . Clin Sci (Lond), 1985; 68(3)：341.
13.	尹珊珊，王宝恩，贾继东，等. 胆管阻塞性肝纤维化模型的建立和改进 [Ｊ] . 中华肝脏病杂志， 2002； 10(5)：385.
14.	Nithya M, Suguna L, Rose C. The effect of nerve growth factor on the early responses during the process of wound healing [Ｊ] . Biochim Biophys Acta, 2003; 1620(1-3)：25.
15.	Micera A, Lambiase A, Stampachiacchiere B, et al . Nerve growth factor and tissue repair remodeling: trkA(NGFR) and p75(NTR), two receptors one fate [Ｊ] . Cytokine Growth Factor Rev, 2007; 18(3-4)：245.

1. Guyot C, Combe C, Desmoulière A. The common bile duct ligation in rat: A relevant in vivo model to study the role of mechanical stress on cell and matrix behaviour [Ｊ] . Histochem Cell Biol, 2006; 126(4)：517.
2. Gigliozzi A, Alpini G, Baroni GS, et al . Nerve growth factor modulates the proliferative capacity of the intrahepatic biliary epithelium in experimental cholestasis [Ｊ] . Gastroenterology, 2004; 127(4)：1198.
3. Aloe L, Bracci-Laudiero L, Bonini S, et al . The expanding role of nerve growth factor: from neurotrophic activity to immunologic diseases [Ｊ] . Allergy, 1997; 52(9)：883.
4. Freund-Michel V, Frossard N. The nerve growth factor and its receptors in airway inflammatory diseases [Ｊ] . Pharmacol Ther, 2008; 117(1)：52.
5. Alvaro D, Mancino MG, Onori P, et al . Estrogens and the pathophysiology of the biliary tree [Ｊ] . World J Gastroenterol, 2006; 12(22)：3537.
6. Takahashi H, Uno S, Watanabe Y, et al . Expression of nerve growth factor-induced type 1 plasminogen activator inhibitor (PAI-1) mRNA is inhibited by genistein and wortmannin [Ｊ] . Neuroreport, 2000; 11(5)：1111.
7. Olgart Hglund C, de Blay F, Oster JP, et al . Nerve growth factor levels and localisation in human asthmatic bronchi [Ｊ] . Eur Respir J, 2002; 20(5)：1110.
8. Freund-Michel V, Bertrand C, Frossard N. TrkA signalling pathways in human airway smooth muscle cell proliferation [Ｊ] . Cell Signal, 2006; 18(5)：621.
9. Levi-Montalcini R. The nerve growth factor 35 years later [Ｊ] . Science, 1987; 237(4819)：1154.
10. 尹兰宁，侯治军，宋爱琳，等. 良性梗阻性黄疸患者血清中IGF-1变化的意义 [Ｊ] . 中国普外基础与临床杂志， 2005; 12(4)：349.
11. 刘双海，蔡景修，顾红光，等. 炎性狭窄胆管壁中成纤维细胞表型改变及增殖的意义 [Ｊ] . 中国普外基础与临床杂志， 2000; 7(2)：69.
12. Kountouras J, Scheuer PJ, Billing BH. Effect of prolonged bile duct obstruction in the rat on hepatic transport of bilirubin [Ｊ] . Clin Sci (Lond), 1985; 68(3)：341.
13. 尹珊珊，王宝恩，贾继东，等. 胆管阻塞性肝纤维化模型的建立和改进 [Ｊ] . 中华肝脏病杂志， 2002； 10(5)：385.
14. Nithya M, Suguna L, Rose C. The effect of nerve growth factor on the early responses during the process of wound healing [Ｊ] . Biochim Biophys Acta, 2003; 1620(1-3)：25.
15. Micera A, Lambiase A, Stampachiacchiere B, et al . Nerve growth factor and tissue repair remodeling: trkA(NGFR) and p75(NTR), two receptors one fate [Ｊ] . Cytokine Growth Factor Rev, 2007; 18(3-4)：245.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expressions and Significance of Nerve Growth Factor and Its High Affinity Receptor in Connective Tissue of Bile Duct

Abstract Full text Figures/Tables Video References Cited by

Format

Content