Experimental Study of Fe 3+ -Modified Carboxymethyl Cellulose Preventing Postoperative Peritoneal Adhesion and Inhibiting The Expressions of TNF-α and FGF in Injured Part of Postoperative Peritoneum_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YU Jinhai , SUO Jian , LI Youzhu , LIU Guohui , CHEN Yan , WANG Quan

* Department of General Surgery, The First Hospital of Jilin University, Changchun 130021, ChinaCorresponding Author: SUO Jian， E-mail： suojian0066@yahoo.com.cn;

Corresponding author：

Keywords：

Fe 3+ -modified carboxy methyl cellulose; Tumor necrosis factor-α; Fibroblast growth factor; Peritoneal adhesion

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Objective To study the effect of Fe 3+ -modified carborymethyl celluiose (Fe 3+ -CMC ) on preventing postoperative adhesion and inhibiting the expressions of tumor necrosis factor-α (TNF-α) and fibroblast growth factor (FGF) in the injured parts of postoperative peritoneum. Methods Fourty Wistar mice were divided into 2 groups randomly, and abdominal adhesion models were made, then 0.9% NaCl (control group) and Fe 3+ -CMC (experimental group) were sprayed into the wound surface of abdominal cavity. All mice were killed to observe the adhesion condition on day 14 after operation. Another 120 Wistar mice were divided into 2 groups randomly, and abdominal adhesion models were made as mentioned above. Ten mice were killed which were chosen randomly from 2 groups on day 1, 3, 5, 7, 14 and 60, respectively. The expressions of TNF-α and FGF in the peritoneal injured and adhesion tissues were observed by immunohistochemistry technique. Results The adhesion grade in experimental group was much lower than that in control group ( P ＜ 0.01). The expression of TNF-α (day 3-7 after operation) and FGF (day 5-7 after operation) in experimental group were lower than those in control group ( P ＜ 0.05).
Conclusion Fe 3+ -CMC can decrease postoperative adhesion grade and prevent the expressions of TNF-α and FGF in injured parts of postoperative peritoneum.

Citation： YU Jinhai,SUO Jian,LI Youzhu,LIU Guohui,CHEN Yan,WANG Quan,.. Experimental Study of Fe 3+ -Modified Carboxymethyl Cellulose Preventing Postoperative Peritoneal Adhesion and Inhibiting The Expressions of TNF-α and FGF in Injured Part of Postoperative Peritoneum. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(1): 52-55. doi: Copy

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1. 1 吴立格，于金海，葛岩. 羧甲基纤维素、几丁糖预防腹膜粘连的实验研究 [J] . 中国普外基础与临床杂志, 2005; 12（6）：前插 2 田易军, 胡森, 包呈梅, 等. 卡巴胆碱预防术后腹腔粘连的实验研究 [J] . 中国普外基础与临床杂志, 2008; 15(6)：435.
2. 翁永强. β-转移生长因子与腹膜粘连 [J] . 国外医学·外科学分册， 2000; 27（2）：94.
3. 刘国辉, 李有柱，赵军. 羧甲基纤维素钠在预防术后腹膜粘连中的作用 [J] . 吉林大学学报（医学版）, 2002; 28（2）：163.
4. 胡建敏, 黄绍贤, 候忠民, 等. 人体脂肪、α-糜蛋白酶联合腹腔灌注预防粘连的实验及临床研究 [J] . 中华实验外科杂志, 1989; 6(3)：101.
5. Ghellai AM, Stucchi AF, Chegini N, et al . Role of transforming growth factor beta-1 in peritonitis-induced adhesions [J] . J Gastrointest Surg, 2000; 4(3)：316.
6. Selman M, Thannickal VJ, Pardo A, et al. Idiopathic pulmonary fibrosis: pathogenesis and therapeutic approaches [J] . Drugs, 2004; 64(4)：405.
7. Wright TJ, Hatch EP, Karabagli H, et al . Expression of mouse fibroblast growth factor and fibroblast growth factor receptor genes during early inner ear development [J] . Dev Dyn, 2003; 228(2)：267.
8. Mirastschijski U, Johannesson K, Jeppsson B， et al. Effect of a matrix metalloproteinase activity and TNF-alpha converting enzyme inhibitor on intra-abdominal adhesions [J] . Eur Surg Res, 2005; 37(1)：68.
9. Roy S, Clark CJ, Mohebali K, et al . Reactive oxygen species and EGR-1 gene expression in surgical postoperative peritoneal adhesions [J] . World J Surg, 2004; 28(3)：316.
10. Aubé C, Pessaux P, Tuech JJ, et al . Detection of peritoneal adhesions using ultrasound examination for the evaluation of an innovative intraperitoneal mesh [J] . Surg Endosc, 2004; 18 (1)： 131.
11. 张平，杜晓宏，杨斌. 聚己内酯隔离膜防止大鼠术后腹腔粘连作用的研究 [J] . 中国普外基础与临床杂志, 2006; 13 （2）： 194.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Study of Fe 3+ -Modified Carboxymethyl Cellulose Preventing Postoperative Peritoneal Adhesion and Inhibiting The Expressions of TNF-α and FGF in Injured Part of Postoperative Peritoneum

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