Effect of Substance P on Intracellular Free Calcium Concentration in Cultured Gastric Cancer Cell in Vit ro_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

GUAN Chen , ZHANG Hongwei , WAN G Weizhong , SONG Junfeng , FENG Fan , JI Gang

Department of Gastrointestinal Surgery , Xijing Hospital , The Fourth Military Medical Universi t y , Xi’an 710032 , China;

Corresponding author：

Keywords：

Substance P，Gast ric cancer cell，Calcium，Laser scanning confocal microscope

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective 　To study the effect of substance P ( SP) on int racellular f ree calcium concent ration in human poorly-differentiated gast ric cancer cell in vitro. Methods 　Human gast ric cancer cell line MKN45 was cultured in RPMI 1640. Then the cells were loaded with specific calcium fluorescent probe Furu23/ AM. ASN21377642 (NK21 receptor antagonist) , Nicardipine (calcium channel blocker) and different concent rations of SP were used to treat gast ric cancer cells. The concent ration changes of int racellular free calcium were detected by laser scanning confocal microscope. Results 　It was found that 10 , 50 and 100 nmol/ L SP could significantly increase the int racellular free calcium concent ration of gast ric cancer cells in Hanks solutions , which contain ext racellular calcium ( P lt;0. 05) , and the change was in a dose-dependent manner ( P lt; 0. 05) . When there was ext racellular calcium existed ,the increasing amplitude of intracellular f ree calcium concent ration was significantly higher than that when there was no extracellular calcium ( P lt; 0. 05) . And when Hanks solutions were pretreated with ASN21377642 and Nicardipine , the effects of 100 nmol/ L SP were partly inhibited , and the concent rations of int racellular f ree calcium were significantly lower than those in group s without pret reatment s ( P lt; 0. 05) . Conclusion 　SP can significantly increase free calcium concent ration in the gastric cancer cells. Releasing of stored calcium in the cells and influx of extracelluar calcium may contribute to the elevation of int racellular free calcium concentration.

Citation： GUAN Chen,ZHANG Hongwei,WAN G Weizhong,SONG Junfeng,FENG Fan,JI Gang. Effect of Substance P on Intracellular Free Calcium Concentration in Cultured Gastric Cancer Cell in Vit ro. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(1): 8-11. doi: Copy

1.	Castagliuolo I,Valenick L,Liu J,et al.Epidermal growth factor receptor transactivation mediates substance P-induced mitogenic responses in U-373 MG cells[J].J Biol Chem,2000; 275(34)∶26545.
2.	Corbett EF,Michalak M.Calcium,a signaling molecule in the endoplasmic reticulum?[J].Trends Biochem Sci,2000; 25(7)∶307.
3.	Teruel MN,Chen W,Persechini A,et al.Differential codes for free Ca(2+)-calmodulin signals in nucleus and cytosol[J].Curr Biol,2000; 10(2)∶86.
4.	刘君,李宁,宋少柏.胃癌的分子生物学研究进展[J].中国普外基础与临床杂志,2000; 7(3)∶197.
5.	张多强,李玉民,何晓东,等.黏着斑激酶的高表达与人胃癌侵袭和转移的关系[J].中国普外基础与临床杂志,2006; 13(1)∶43.
6.	张洪伟,王为忠,宋俊峰,等.SP免疫阳性神经纤维在胃癌组织中分布的实验研究人[J].中国现代医学杂志,2005; 15(24)∶3692.
7.	张洪伟,王为忠,宋俊峰,等.人胃癌组织中神经纤维发生可塑性改变的研究[J].中国癌症杂志,2007; 17(3)∶261.
8.	张洪伟,王为忠,宋俊峰,等.P物质对胃癌细胞增殖的作用的研究[J].中华实验外科杂志,2007; 24(6)∶150.
9.	薛少白,张鸿卿,王端顺,等译.细胞增殖的生物学[M].北京:北京大学出版社,1985∶99～123.
10.	Oh EJ,Gover TD,Cordoba-Rodriguez R,et al.Substance P evokes cation currents through TRP channels in HEK293 cells[J].J Neurophysiol,2003; 90(3)∶2069.
11.	Ito K,Rome C,Bouleau Y,et al.Substance P mobilizes intracellular calcium and activates a nonselective cation conductance in rat spiral ganglion neurons[J].Eur J Neurosci,2002; 16(11)∶2095.
12.	Sun W,Ding DL,Wang P,et al.Substance P inhibits potassium and calcium currents in inner ear spiral ganglion neurons[J].Brain Res,2004; 1012(1-2)∶82.
13.	史娟,李继硕.TRP离子通道[J].神经解剖学杂志,2004; 20(2)∶197.
14.	Galiano M,Gasparre G,Lippe C,et al.Calcium response after stimulation by substance P of U373 MG cells:inhibition of store-operated calcium entry by protein kinase C[J].Cell Calcium,2004; 35(2)∶123.

1. Castagliuolo I,Valenick L,Liu J,et al.Epidermal growth factor receptor transactivation mediates substance P-induced mitogenic responses in U-373 MG cells[J].J Biol Chem,2000; 275(34)∶26545.
2. Corbett EF,Michalak M.Calcium,a signaling molecule in the endoplasmic reticulum?[J].Trends Biochem Sci,2000; 25(7)∶307.
3. Teruel MN,Chen W,Persechini A,et al.Differential codes for free Ca(2+)-calmodulin signals in nucleus and cytosol[J].Curr Biol,2000; 10(2)∶86.
4. 刘君,李宁,宋少柏.胃癌的分子生物学研究进展[J].中国普外基础与临床杂志,2000; 7(3)∶197.
5. 张多强,李玉民,何晓东,等.黏着斑激酶的高表达与人胃癌侵袭和转移的关系[J].中国普外基础与临床杂志,2006; 13(1)∶43.
6. 张洪伟,王为忠,宋俊峰,等.SP免疫阳性神经纤维在胃癌组织中分布的实验研究人[J].中国现代医学杂志,2005; 15(24)∶3692.
7. 张洪伟,王为忠,宋俊峰,等.人胃癌组织中神经纤维发生可塑性改变的研究[J].中国癌症杂志,2007; 17(3)∶261.
8. 张洪伟,王为忠,宋俊峰,等.P物质对胃癌细胞增殖的作用的研究[J].中华实验外科杂志,2007; 24(6)∶150.
9. 薛少白,张鸿卿,王端顺,等译.细胞增殖的生物学[M].北京:北京大学出版社,1985∶99～123.
10. Oh EJ,Gover TD,Cordoba-Rodriguez R,et al.Substance P evokes cation currents through TRP channels in HEK293 cells[J].J Neurophysiol,2003; 90(3)∶2069.
11. Ito K,Rome C,Bouleau Y,et al.Substance P mobilizes intracellular calcium and activates a nonselective cation conductance in rat spiral ganglion neurons[J].Eur J Neurosci,2002; 16(11)∶2095.
12. Sun W,Ding DL,Wang P,et al.Substance P inhibits potassium and calcium currents in inner ear spiral ganglion neurons[J].Brain Res,2004; 1012(1-2)∶82.
13. 史娟,李继硕.TRP离子通道[J].神经解剖学杂志,2004; 20(2)∶197.
14. Galiano M,Gasparre G,Lippe C,et al.Calcium response after stimulation by substance P of U373 MG cells:inhibition of store-operated calcium entry by protein kinase C[J].Cell Calcium,2004; 35(2)∶123.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Substance P on Intracellular Free Calcium Concentration in Cultured Gastric Cancer Cell in Vit ro

Abstract Full text Figures/Tables Video References Cited by

Format

Content