Effects of Tiam 1 Antisense Oligonucleotides on Morphological Remodeling of Gastric Cancer Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHU Jinming ¹ , YU Peiw u ¹ , LI Cuifang ² , WU Miao ³ , Z HAO Yongliang ¹

1. Department of General Surgery , Southwest Hospital of The Third Military Medical University , Chongqing 400038 , China;;
2. Department of Internal Medicine ,The Fifth People’s Hospital of Xingtai City , Hebei Province , Xingtai 054027 , China;;
3. Department of General Surgery , The 180th Hospital of Nanjing Military Area of PL A , Quanzhou 362000 , China;

Corresponding author：

Keywords：

Gastric cancer,Tlymphoma invasion and metastasis inducing factor,Antisense oligodeoxynucleotides,Transfection 　　Morphology

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Objective 　To investigate the effect s of T lymphoma invasion and metastasis inducing factor 1 ( Tiam 1) antisense oligonucleotides (ASODN) on morphological remodeling of gast ric cancer cells. Methods 　The high-invasive and metastastic subgroup (MH ) was separated f rom human gast ric cancer cell line MKN245 (M0 ) by laminin adhesion method in vi t ro. And they were divided into four group s according to different further t reatment s : no t ransfection group (cont rol group ) , liposome t ransfection group , sense oligonucleotides2liposome t ransfection group ( SODN t ransfection with liposome group ) and antisense oligonucleotides2liposome t ransfection group (ASODN t ransfection with liposome group) . Then the expressions of Tiam 1 mRNA and protein were detected by RT-PCR and flowcytomet ry , respectively. The morphology changes between Tima 1 ASODN t ransfected MH cells and no t ransfected cells were observed by using HE stain , cytoskeletal protein stain and scanning elect ronic microscope (SEM) . Results 　Compared with the other group s , the expressions of Tiam 1 mRNA and protein in MH cells were significantly decreased af ter the cells were t ransfected with 0. 43 μmol/ L Tiam 1 ASODN ( P lt; 0. 01) . Additionally , it was observed that the t ransfected MH cells had less membrane surface projections , fewer or shortener pseudopodia , less irregular cytoskeletal network and less spotted-like actin bodys than no t ransfected MH cells did. Conclusion 　ASODN t ransfection could effectively suppress the expression of Tiam 1 and the remodeling in gast ric cancer cells , which may play an important role in the invasion and metastasis of gast ric cancer cells.

Citation： ZHU Jinming ,YU Peiw u,LI Cuifang,WU Miao,Z HAO Yongliang. Effects of Tiam 1 Antisense Oligonucleotides on Morphological Remodeling of Gastric Cancer Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(1): 12-16. doi: Copy

1.	<><> [1]Jaeken L.A new list of functions of the cytoskeleton[J].IUBMB Life,2007; 59(3)∶127.
2.	Minard ME,Ellis LM,Gallick GE.Tiam 1 regulates cell adhesion,migration and apoptosis in colon tumor cells[J].Clin Exp Metastasis,2006; 23(5-6)∶301.
3.	朱金明,余佩武.Tiam 1表达与胃癌侵袭转移关系的临床和实验研究[J].临床与实验病理学杂志,2006; 22(1)∶85.
4.	王舒宝,王俊.胃癌复发与转移的有关问题及综合治疗[J].中国普外基础与临床杂志,2006; 13(1)∶9.
5.	陈向荣,任卫平,童菊芳,等.粘附法筛选胃癌细胞亚株[J].胃肠病学,2000; 5(2)∶116.
6.	张进华,丁彦青,杨学皆.介绍一种细胞骨架蛋白改良染色法[J].诊断病理学杂志,1996; 3(4)∶235.
7.	Mertens AE,Roovers RC,Collard JG.Regulation of Tiam 1-Rac signalling[J].FEBS Lett,2003; 546(1)∶11.
8.	Strumane K,Rygiel TP,Collard JG.The rac activator tiam 1 and ras-induced oncogenesis[J].Methods Enzymol,2005; 407∶269.
9.	Yamauchi J,Miyamoto Y,Tanoue A,et al.Ras activation of a Rac 1 exchange factor,Tiam 1,mediates neurotrophin-3-induced Schwann cell migration[J].Proc Natl Acad Sci USA,2005; 102(41)∶14889.
10.	Liu L,Zhao L,Zhang Y,et al.Proteomic analysis of Tiam 1-mediated metastasis in colorectal cancer[J].Cell Biol Int,2007; 31(8)∶805.
11.	Engers R,Mueller M,Walter A,et al.Prognostic relevance of Tiam 1 protein expression in prostate carcinomas[J].Br J Cancer,2006; 95(8)∶1081.
12.	Minard ME,Kim LS,Price JE,et al.The role of the guanine nucleotide exchange factor Tiam 1 in cellular migration,invasion,adhesion and tumor progression[J].Breast Cancer Res Treat,2004; 84(1)∶21.
13.	Pawlak G,Helfman DM.Cytoskeletal changes in cell transformation and tumorigenesis[J].Curr Opin Genet Dev,2001; 11(1)∶41.
14.	Engers R,Gabbert HE.Mechanisms of tumor metastasis:cell biological aspects and clinical implications[J].J Cancer Res Clin Oncol,2000; 126(12)∶682.
15.	Lleonart ME,Martin-Duque P,Sanchez-Prieto R,et al.Tum-or heterogeneity:morphological,molecular and clinical implications[J].Histol Histopathol,2000; 15(3)∶881.
16.	Hoenger A,Nicastro D.Electron microscopy of microtubule-ba-sed cytoskeletal machinery[J].Methods Cell Biol,2007; 79∶437.
17.	Lenormand G,Fredberg JJ.Deformability,dynamics,and remodeling of cytoskeleton of the adherent living cell[J].Biorheology,2006; 43(1)∶1.
18.	Keely PJ,Westwick JK,Whitehead IP,et al.Cdc42 and Rac 1 induce integrin-mediated cell motility and invasiveness through PI(3)K[J].Nature,1997; 390(6660)∶632.
19.	Delon I,Brown NH.Integrins and the actin cytoskeleton[J].Curr Opin Cell Biol,2007; 19(1)∶43.
20.	Bourguignon LY,Zhu H,Shao L,et al.CD44 interaction with tiam 1 promotes Rac 1 signaling and hyaluronic acid-mediated breast tumor cell migration[J].J Biol Chem,2000; 275(3)∶1829.
21.	徐胜军,胡炳奎,凌志强,等.CD44v6在胃癌中的表达及其临床意义[J].中国普外基础与临床杂志,2006; 13(1)∶42.

1. <><> [1]Jaeken L.A new list of functions of the cytoskeleton[J].IUBMB Life,2007; 59(3)∶127.
2. Minard ME,Ellis LM,Gallick GE.Tiam 1 regulates cell adhesion,migration and apoptosis in colon tumor cells[J].Clin Exp Metastasis,2006; 23(5-6)∶301.
3. 朱金明,余佩武.Tiam 1表达与胃癌侵袭转移关系的临床和实验研究[J].临床与实验病理学杂志,2006; 22(1)∶85.
4. 王舒宝,王俊.胃癌复发与转移的有关问题及综合治疗[J].中国普外基础与临床杂志,2006; 13(1)∶9.
5. 陈向荣,任卫平,童菊芳,等.粘附法筛选胃癌细胞亚株[J].胃肠病学,2000; 5(2)∶116.
6. 张进华,丁彦青,杨学皆.介绍一种细胞骨架蛋白改良染色法[J].诊断病理学杂志,1996; 3(4)∶235.
7. Mertens AE,Roovers RC,Collard JG.Regulation of Tiam 1-Rac signalling[J].FEBS Lett,2003; 546(1)∶11.
8. Strumane K,Rygiel TP,Collard JG.The rac activator tiam 1 and ras-induced oncogenesis[J].Methods Enzymol,2005; 407∶269.
9. Yamauchi J,Miyamoto Y,Tanoue A,et al.Ras activation of a Rac 1 exchange factor,Tiam 1,mediates neurotrophin-3-induced Schwann cell migration[J].Proc Natl Acad Sci USA,2005; 102(41)∶14889.
10. Liu L,Zhao L,Zhang Y,et al.Proteomic analysis of Tiam 1-mediated metastasis in colorectal cancer[J].Cell Biol Int,2007; 31(8)∶805.
11. Engers R,Mueller M,Walter A,et al.Prognostic relevance of Tiam 1 protein expression in prostate carcinomas[J].Br J Cancer,2006; 95(8)∶1081.
12. Minard ME,Kim LS,Price JE,et al.The role of the guanine nucleotide exchange factor Tiam 1 in cellular migration,invasion,adhesion and tumor progression[J].Breast Cancer Res Treat,2004; 84(1)∶21.
13. Pawlak G,Helfman DM.Cytoskeletal changes in cell transformation and tumorigenesis[J].Curr Opin Genet Dev,2001; 11(1)∶41.
14. Engers R,Gabbert HE.Mechanisms of tumor metastasis:cell biological aspects and clinical implications[J].J Cancer Res Clin Oncol,2000; 126(12)∶682.
15. Lleonart ME,Martin-Duque P,Sanchez-Prieto R,et al.Tum-or heterogeneity:morphological,molecular and clinical implications[J].Histol Histopathol,2000; 15(3)∶881.
16. Hoenger A,Nicastro D.Electron microscopy of microtubule-ba-sed cytoskeletal machinery[J].Methods Cell Biol,2007; 79∶437.
17. Lenormand G,Fredberg JJ.Deformability,dynamics,and remodeling of cytoskeleton of the adherent living cell[J].Biorheology,2006; 43(1)∶1.
18. Keely PJ,Westwick JK,Whitehead IP,et al.Cdc42 and Rac 1 induce integrin-mediated cell motility and invasiveness through PI(3)K[J].Nature,1997; 390(6660)∶632.
19. Delon I,Brown NH.Integrins and the actin cytoskeleton[J].Curr Opin Cell Biol,2007; 19(1)∶43.
20. Bourguignon LY,Zhu H,Shao L,et al.CD44 interaction with tiam 1 promotes Rac 1 signaling and hyaluronic acid-mediated breast tumor cell migration[J].J Biol Chem,2000; 275(3)∶1829.
21. 徐胜军,胡炳奎,凌志强,等.CD44v6在胃癌中的表达及其临床意义[J].中国普外基础与临床杂志,2006; 13(1)∶42.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Tiam 1 Antisense Oligonucleotides on Morphological Remodeling of Gastric Cancer Cells

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