Detection of Multiple Gene Mutations in Stool for Secondary Screening for Colorectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

QIN Gaoping , WANG Xiaoqiang , YAN Likun , SONG Yong

Department of General Surgery, Shaanxi Provincial People’s Hospital, Xi’an 710068, China;

Corresponding author：

Keywords：

Colorectal cancer; Stool; Multiple gene detection

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective 　To evaluate the possibility of detection mutations of 〖JP2〗multiple genes in stool for secondary screening for colorectal cancer.
Methods 　Tumor specimens and stool samples from 40 patients with colorectal cancer and 40 normal persons were examined for mutations of p53, K-ras and APC gene by polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) and silver nitrate staining.
Results 　①The mutation rate of p53, K-ras and APC gene in the tissues and stools of colorectal cancer respectively were 57.50%, 50.00%, 60.00% and 42.86%, 40.00%, 51.43%, and no mutations were found in normal mucosa and stool. ②The mutation ratioes between multiple gene and single gene had significant difference (P＜0.05). ③The sensitivities had no significant difference between faecal occult blood test (FOBT) and multiple gene mutations detection in the diagnosis of colorectal cancer, but the specificity of the latter was higher than FOBT (P＜0.05).
Conclusion 　 Detection of multiple gene mutations in stool is a vauble method in the secondary screening for colorectal cancer.

Citation： QIN Gaoping,WANG Xiaoqiang,YAN Likun,SONG Yong. Detection of Multiple Gene Mutations in Stool for Secondary Screening for Colorectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(6): 639-643. doi: Copy

1.	Price AS. Primary and secondary prevention of colorectal cancer [Ｊ]. Gastroenterol Nurs， 2003; 26(2)∶73.
2.	贺立绩，王桂芳，黄瑞德，等． 1990 年－1992 年山西阳城居民恶性肿瘤死亡情况分析 [Ｊ]. 肿瘤研究与临床, 2000; 12（6）∶430.
3.	陈建顺，陈礼慈，游建旺，等．长乐市中年人恶性肿瘤死亡分析 [Ｊ]. 　中国肿瘤, 2001; 10(9)∶514.
4.	林金荣, 林鸿, 周殿元, 等. 应用银染PCR-SSCP检测结、直肠癌组织及粪便抑癌基因点突变 [Ｊ]. 中华流行病杂志, 1998,19(5)∶B326.
5.	Nollau P, Moser C, Weinland G, et al. Detection of K-ras mutations in stools of patients with colorectal cancer by mutant-enriched PCR [Ｊ]. Int J Cancer, 1996; 66(3)∶332.
6.	Jais P, Kapel N, Chosidow D, et al. Detection of protein p53 in the stool of patients with colorectal cancer [Ｊ]. Gastroenterol Clin Biol, 1997; 21(10)∶754.
7.	Hasegawa H, Ueda M, Furukawa K, et al. p53 gene mutations in early colorectal carcinoma. De novo vs. adenoma-carcinoma sequence [Ｊ]. Int J Cancer, 1995; 64(1)∶47.
8.	胡成平, 郑智远, 杨红忠, 等. 癌性胸腔积液K-ras基因突变检测及临床应用 [Ｊ]. 中国现代医学杂志, 1999; 9(11)∶5.
9.	Strul H, Arber N. Fecal occult blood test for colorectal cancer screening [Ｊ]. Ann Oncol, 2002; 13(1)∶51.
10.	Cooper GS, Yuan Z, Veri L, et al. Colorectal carcinoma sc-reening attitudes and practices among primary care physicians in counties at extremes of either high or low cancer case-fatality [Ｊ]. Cancer, 1999; 86(9)∶1669.
11.	Ahlquist DA. Fecal occult blood testing for colorectal cancer. Can we afford to do this? [Ｊ]. Gastroenterol Clin North Am, 1997; 26(1)∶41.
12.	Sidransky D, Tokino T, Hamilton SR, et al. Identification of ras oncogene mutations in the stool of patients with curable colorectal tumors [Ｊ]. Science, 1992; 256(5053)∶102.
13.	Ratto C, Flamini G, Sofo L, et al. Detection of oncogene mutation from neoplastic colonic cells exfoliated in feces [Ｊ]. Dis Colon Rectum, 1996; 39(11)∶1238.
14.	范如英，李世荣，吴霞，等．粪便大肠癌脱落细胞形态学观察、 DNA定量及p53蛋白表达的初步研究 [Ｊ]. 中华消化杂志, 2000; 20(4)∶269.
15.	Nollau P, Moser C, Weinland G, et al. Detection of K-ras mutations in stools of patients with colorectal cancer by mutant-enriched PCR [Ｊ]. Int J Cancer, 1996; 66(3)∶332.
16.	倪升发. K-ras基因与大肠癌关系的研究进展 [Ｊ]. 医学综述， 2006； 12（1）∶15.
17.	徐亮，卢光宇. 大肠癌ras p21表达和DNA含量定量分析 [Ｊ]. 中国普外基础与临床杂志， 1998； 5（1）∶4.
18.	高枫，唐卫中，李卫. 中国人散发性大肠癌K-ras基因突变的研究 [Ｊ]. 中华实验外科杂志， 2005； 22（1）∶65.
19.	Beranek M, Bures J, Palicka V, et al. A relationship between K-ras gene mutations and some clinical and histologic variables in patients with primary colorectal carcinoma [Ｊ]. Clin Chem Lab Med, 1999; 37(7)∶723.
20.	王一理，王剑利，李琳琳. 癌基因·抑癌基因·肿瘤相关基因 [A]. 见：司履生主编. 癌基因抑癌基因肿瘤相关基因 [M]. 第1版. 西安：陕西科学技术出版社, 2002∶63.

1. 　Price AS. Primary and secondary prevention of colorectal cancer [Ｊ]. Gastroenterol Nurs， 2003; 26(2)∶73.
2. 　贺立绩，王桂芳，黄瑞德，等． 1990 年－1992 年山西阳城居民恶性肿瘤死亡情况分析 [Ｊ]. 肿瘤研究与临床, 2000; 12（6）∶430.
3. 　陈建顺，陈礼慈，游建旺，等．长乐市中年人恶性肿瘤死亡分析 [Ｊ]. 　中国肿瘤, 2001; 10(9)∶514.
4. 　林金荣, 林鸿, 周殿元, 等. 应用银染PCR-SSCP检测结、直肠癌组织及粪便抑癌基因点突变 [Ｊ]. 中华流行病杂志, 1998,19(5)∶B326.
5. 　Nollau P, Moser C, Weinland G, et al. Detection of K-ras mutations in stools of patients with colorectal cancer by mutant-enriched PCR [Ｊ]. Int J Cancer, 1996; 66(3)∶332.
6. 　Jais P, Kapel N, Chosidow D, et al. Detection of protein p53 in the stool of patients with colorectal cancer [Ｊ]. Gastroenterol Clin Biol, 1997; 21(10)∶754.
7. 　Hasegawa H, Ueda M, Furukawa K, et al. p53 gene mutations in early colorectal carcinoma. De novo vs. adenoma-carcinoma sequence [Ｊ]. Int J Cancer, 1995; 64(1)∶47.
8. 　胡成平, 郑智远, 杨红忠, 等. 癌性胸腔积液K-ras基因突变检测及临床应用 [Ｊ]. 中国现代医学杂志, 1999; 9(11)∶5.
9. 　Strul H, Arber N. Fecal occult blood test for colorectal cancer screening [Ｊ]. Ann Oncol, 2002; 13(1)∶51.
10. 　Cooper GS, Yuan Z, Veri L, et al. Colorectal carcinoma sc-reening attitudes and practices among primary care physicians in counties at extremes of either high or low cancer case-fatality [Ｊ]. Cancer, 1999; 86(9)∶1669.
11. 　Ahlquist DA. Fecal occult blood testing for colorectal cancer. Can we afford to do this? [Ｊ]. Gastroenterol Clin North Am, 1997; 26(1)∶41.
12. 　Sidransky D, Tokino T, Hamilton SR, et al. Identification of ras oncogene mutations in the stool of patients with curable colorectal tumors [Ｊ]. Science, 1992; 256(5053)∶102.
13. 　Ratto C, Flamini G, Sofo L, et al. Detection of oncogene mutation from neoplastic colonic cells exfoliated in feces [Ｊ]. Dis Colon Rectum, 1996; 39(11)∶1238.
14. 　范如英，李世荣，吴霞，等．粪便大肠癌脱落细胞形态学观察、 DNA定量及p53蛋白表达的初步研究 [Ｊ]. 中华消化杂志, 2000; 20(4)∶269.
15. 　Nollau P, Moser C, Weinland G, et al. Detection of K-ras mutations in stools of patients with colorectal cancer by mutant-enriched PCR [Ｊ]. Int J Cancer, 1996; 66(3)∶332.
16. 　倪升发. K-ras基因与大肠癌关系的研究进展 [Ｊ]. 医学综述， 2006； 12（1）∶15.
17. 　徐亮，卢光宇. 大肠癌ras p21表达和DNA含量定量分析 [Ｊ]. 中国普外基础与临床杂志， 1998； 5（1）∶4.
18. 　高枫，唐卫中，李卫. 中国人散发性大肠癌K-ras基因突变的研究 [Ｊ]. 中华实验外科杂志， 2005； 22（1）∶65.
19. 　Beranek M, Bures J, Palicka V, et al. A relationship between K-ras gene mutations and some clinical and histologic variables in patients with primary colorectal carcinoma [Ｊ]. Clin Chem Lab Med, 1999; 37(7)∶723.
20. 　王一理，王剑利，李琳琳. 癌基因·抑癌基因·肿瘤相关基因 [A]. 见：司履生主编. 癌基因抑癌基因肿瘤相关基因 [M]. 第1版. 西安：陕西科学技术出版社, 2002∶63.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Detection of Multiple Gene Mutations in Stool for Secondary Screening for Colorectal Cancer

Abstract Full text Figures/Tables Video References Cited by

Format

Content