Evaluated on Expression of Endothelin-3 mRNA Detected by RT-PCR in Acute Pancreatitis Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Yong ¹ , GUO Yongzhang ² , LI Li ³ , CAO Xinan ⁴

Department of Surgery, The First People’s Hospital of Changde City, Hunan Province, Changde 415000, China;

Corresponding author：

Keywords：

Endothelin-3; mRNA; Pancreatitis; Microcirculation

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective 　To investigate the relationship between gene expression of endothelin-3 (ET-3） and inflammation of acute pancreatitis （AP） in rats.
Methods 　Fifty-four rats were divided randomly into 4 groups: sham operation group, AP group, arterial injection group and vein injection group. AP was induced by reverse intra-bile duct infusion 4.5% sodium taurocholate, treated with low dose dopamine 〔5 μg/（kg·min）〕 by injecting arterial or tail vein. Rats were sacrificed at 1， 6 and 24 h after the induction of AP. The mRNA expression of ET-3 was evaluated by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and pathological changes was observed in rats.
Results 　Expression of ET-3 mRNA could be detected from 1 up to 24 h after the induction of pancreatitis. Expression of ET-3 mRNA of sham operation group was decreased significantly compared with other three groups. Expression of ET-3 mRNA showed a significant decrease by arterial injection dopamine than that by tail vein (P＜0.05， P＜0.01). The pathologic score in AP group was the highest, vein injection group was the next one, and score in sham operation group was the lowest.
Conclusion 　There are significant relationship between inflammation of AP and expression of ET-3 mRNA. Dopamine administration by arterial injection is more effective than that by tail vein injection.

Citation： ZHANG Yong,GUO Yongzhang,LI Li,CAO Xinan. Evaluated on Expression of Endothelin-3 mRNA Detected by RT-PCR in Acute Pancreatitis Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(6): 665-669. doi: Copy

1.	瞿建国, 张建新, 程国祚. 急性坏死性胰腺炎胰外器官微循环改变及乌司他丁的影响 [J]. 中国普外基础与临床杂志， 2003， 10(3)∶234.
2.	石湘云, 姚松朝, 杨晔主编. 体液因素与临床疾病 [M]. 第1版. 北京: 北京医科大学中国协和医科大学联合出版社, 1997∶122～127.
3.	Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholate-induced acute haemorrhagic pancreatitis [J]. Scand J Gastroenterol, 1980; 15(4)∶411.
4.	刘全芳，王本茂，唐岩, 等. 小剂量多巴胺对急性出血坏死性胰腺炎大鼠胰腺微血流的影响及其治疗作用 [J]. 普外基础与临床杂志, 1997; 4（4）∶193.
5.	Schmidt J, Lewandrowsi K, Warshaw AL, et al. Morphometric characteristics and homogeneity of a new model of acute pancreatitis in the rat [J]. Int J Pancreatol, 1992; 12(1)∶41.
6.	Nudel U, Zakut R, Shani M, et al. The nucleotide sequence of the rat cytoplasmic beta-actin gene [J]. Nucleic Acids Res, 1983; 11(6)∶1759.
7.	Shigematsu T, Miura S, Hirokawa M, et al. Induction of endothelin-1 synthesis by IL-2 and its modulation of rat intestinal epithelial cell growth [J]. Am J Physiol, 1998; 275(3 Pt 1)∶G556.
8.	Arai H, Hori S, Aramori I, et al. Cloning and expression of a cDNA encoding an endothelin receptor [J]. Nature, 1990; 348(6303)∶730.
9.	Sakurai T, Yanagisawa M, Takuwa Y, et al. Cloning of a cDNA encoding a non-isopeptide-selective subtype of the endothelin receptor [J]. Nature, 1990; 348(6303)∶732.
10.	Loffler BM, Kalina B, Kunze H. Partial characterisation and subcellular distribution patterns of endothelin-1, -2 and -3 binding sites in human liver [J]. Biochem Biophys Res Commun, 1991; 181(2)∶840.
11.	蒲青凡, 严律南, 孙碎康, 等. 改善胰腺缺血和钙拮抗剂在急性重症胰腺炎治疗中的保护作用 [J]. 中国普外基础与临床杂志, 2001; 8(6)∶384.
12.	陈晓理, 黄兴兰, 吴浩. 急性胰腺炎血中抗炎症细胞因子的意义 [J]. 中国普外基础与临床杂志, 2000; 7(6)∶354.
13.	Kuwaki T, Ishii T, Ju K, et al. Blood pressure of endothelin-3 null (-/-) knockout mice and endothelin A receptor null (-/-) knockout mice under anaesthesia [J]. Clin Sci (Lond)， 2002; 103（Suppl 48）∶48S.
14.	Nakamura A, Kuwaki T, Kuriyama T, et al. Normal ventilation and ventilatory responses to chemical stimuli in juvenile mutant mice deficient in endothelin-3 [J]. Respir Physiol， 2001; 124(1)∶1.
15.	Schiffrin EL. Endothelin: potential role in hypertension and va-scular hypertrophy [J]. Hypertension, 1995; 25(6)∶1135.
16.	Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction [J]. Anal Biochem， 1987; 162(1)∶156.
17.	袁晟光，张肇达，刘续宝, 等. 内皮素在大鼠急性胰腺炎发病中的作用 [J]. 中国普外基础与临床杂志, 2000; 7(3)∶150.
18.	姚有贵，林致远，蒋力生，等. 8种抗菌药物透入犬胰组织能力的实验研究 [J]. 中国普外基础与临床杂志, 1999; 6(2)∶76.
19.	Nakata T, Takeda K, Hatta T, et al. Blockade of angiotensin Ⅱ receptors inhibits the increase in blood pressure induced by insulin [J]. J Cardiovasc Pharmacol, 1998; 31(2)∶248.
20.	Aigner KR. Intra-arterial infusion: overview and novel approaches [J]. Semin Surg Oncol, 1998; 14(3)∶248.
21.	蒲青凡, 张川蓉, 严律南, 等. 异搏定区域动脉灌注在阻止急性胰腺炎重症化治疗中的作用 [J]. 中国普外基础与临床杂志, 2007; 14(2)∶200.
22.	周斌，姚南，李玉民，等. 内皮素在梗阻性黄疸肝、肾、心组织损伤中的作用及血塞通干预 [J]. 中国普外基础与临床杂志, 2006； 13(1)∶77.
23.	张丰深，何振平, 段恒春, 等. 阻塞性黄疸大鼠肾脏自分泌内皮素的变化及其与肾功能损害的关系 [J]. 中国普外基础与临床杂志, 2003； 10(1)∶22.

1. 　瞿建国, 张建新, 程国祚. 急性坏死性胰腺炎胰外器官微循环改变及乌司他丁的影响 [J]. 中国普外基础与临床杂志， 2003， 10(3)∶234.
2. 　石湘云, 姚松朝, 杨晔主编. 体液因素与临床疾病 [M]. 第1版. 北京: 北京医科大学中国协和医科大学联合出版社, 1997∶122～127.
3. 　Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholate-induced acute haemorrhagic pancreatitis [J]. Scand J Gastroenterol, 1980; 15(4)∶411.
4. 　刘全芳，王本茂，唐岩, 等. 小剂量多巴胺对急性出血坏死性胰腺炎大鼠胰腺微血流的影响及其治疗作用 [J]. 普外基础与临床杂志, 1997; 4（4）∶193.
5. 　Schmidt J, Lewandrowsi K, Warshaw AL, et al. Morphometric characteristics and homogeneity of a new model of acute pancreatitis in the rat [J]. Int J Pancreatol, 1992; 12(1)∶41.
6. 　Nudel U, Zakut R, Shani M, et al. The nucleotide sequence of the rat cytoplasmic beta-actin gene [J]. Nucleic Acids Res, 1983; 11(6)∶1759.
7. 　Shigematsu T, Miura S, Hirokawa M, et al. Induction of endothelin-1 synthesis by IL-2 and its modulation of rat intestinal epithelial cell growth [J]. Am J Physiol, 1998; 275(3 Pt 1)∶G556.
8. 　Arai H, Hori S, Aramori I, et al. Cloning and expression of a cDNA encoding an endothelin receptor [J]. Nature, 1990; 348(6303)∶730.
9. 　Sakurai T, Yanagisawa M, Takuwa Y, et al. Cloning of a cDNA encoding a non-isopeptide-selective subtype of the endothelin receptor [J]. Nature, 1990; 348(6303)∶732.
10. 　Loffler BM, Kalina B, Kunze H. Partial characterisation and subcellular distribution patterns of endothelin-1, -2 and -3 binding sites in human liver [J]. Biochem Biophys Res Commun, 1991; 181(2)∶840.
11. 　蒲青凡, 严律南, 孙碎康, 等. 改善胰腺缺血和钙拮抗剂在急性重症胰腺炎治疗中的保护作用 [J]. 中国普外基础与临床杂志, 2001; 8(6)∶384.
12. 　陈晓理, 黄兴兰, 吴浩. 急性胰腺炎血中抗炎症细胞因子的意义 [J]. 中国普外基础与临床杂志, 2000; 7(6)∶354.
13. 　Kuwaki T, Ishii T, Ju K, et al. Blood pressure of endothelin-3 null (-/-) knockout mice and endothelin A receptor null (-/-) knockout mice under anaesthesia [J]. Clin Sci (Lond)， 2002; 103（Suppl 48）∶48S.
14. 　Nakamura A, Kuwaki T, Kuriyama T, et al. Normal ventilation and ventilatory responses to chemical stimuli in juvenile mutant mice deficient in endothelin-3 [J]. Respir Physiol， 2001; 124(1)∶1.
15. 　Schiffrin EL. Endothelin: potential role in hypertension and va-scular hypertrophy [J]. Hypertension, 1995; 25(6)∶1135.
16. 　Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction [J]. Anal Biochem， 1987; 162(1)∶156.
17. 　袁晟光，张肇达，刘续宝, 等. 内皮素在大鼠急性胰腺炎发病中的作用 [J]. 中国普外基础与临床杂志, 2000; 7(3)∶150.
18. 　姚有贵，林致远，蒋力生，等. 8种抗菌药物透入犬胰组织能力的实验研究 [J]. 中国普外基础与临床杂志, 1999; 6(2)∶76.
19. 　Nakata T, Takeda K, Hatta T, et al. Blockade of angiotensin Ⅱ receptors inhibits the increase in blood pressure induced by insulin [J]. J Cardiovasc Pharmacol, 1998; 31(2)∶248.
20. 　Aigner KR. Intra-arterial infusion: overview and novel approaches [J]. Semin Surg Oncol, 1998; 14(3)∶248.
21. 　蒲青凡, 张川蓉, 严律南, 等. 异搏定区域动脉灌注在阻止急性胰腺炎重症化治疗中的作用 [J]. 中国普外基础与临床杂志, 2007; 14(2)∶200.
22. 　周斌，姚南，李玉民，等. 内皮素在梗阻性黄疸肝、肾、心组织损伤中的作用及血塞通干预 [J]. 中国普外基础与临床杂志, 2006； 13(1)∶77.
23. 　张丰深，何振平, 段恒春, 等. 阻塞性黄疸大鼠肾脏自分泌内皮素的变化及其与肾功能损害的关系 [J]. 中国普外基础与临床杂志, 2003； 10(1)∶22.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Evaluated on Expression of Endothelin-3 mRNA Detected by RT-PCR in Acute Pancreatitis Rats

Abstract Full text Figures/Tables Video References Cited by

Format

Content