Study on Dynamic Expression of Hepatic Proliferating Cell Nuclear Antigen in The Occurrence and Development of Hepatocellular Carcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

GONG Jiaqing , FANG Chihua

The PLA Medical Central of General Surgery, General Hospital of Chengdu Command, Chengdu 610083, China;

Corresponding author：

Keywords：

Proliferating cell nuclear antigen; Oval cell; Hepatocellular carcinoma

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Objective 　To study the expression of proliferating cell nuclear antigen (PCNA) in the occurrence and development of hepatocellular carcinoma.
Methods 　Sixty SD rats were randomly divided into control group and experimental group. 3′MeDAB was administrated into rats to establish the experimental model of hepatocarcinoma. The expressions of PCNA of different phases were detected by immunohistochemistry and the liver pathologic changes were observed by optical microscope.
Results 　The process of canceration was divided into three stages: inflammation, proliferative fibrosis and hepatic carcinoma. The expression of PCNA firstly presented in the oval cells that located in the portal area at the stage of inflammation, and a part of PCNA were hyperexpressed in the portal area. The expression rate of PCNA in the middle phase of inflammatory stage was higher than that of any other phases but declined later. Yet, when it came to the stage of hepatic carcinoma, the rate increased again.
Conclusion 　Under the experimental circumstance when liver cancer is caused by the carcinogenic agent, PCNA may be firstly expressed in the oval cells, and the dynamic expression of PCNA may be an indicator for the early diagnosis of hepatocarcinogenesis.

Citation： GONG Jiaqing,FANG Chihua. Study on Dynamic Expression of Hepatic Proliferating Cell Nuclear Antigen in The Occurrence and Development of Hepatocellular Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(1): 11-14. doi: Copy

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2. 　Chung JK, Lee T, Jung IM, et al. Expression of cell cycle regulators during smooth muscle cell proliferation after balloon catheter injury of rat artery [J]. J Korean Med Sci, 2004; 19(3)∶327.
3. 　Guettier C. Which stem cells for adult liver? [J].Ann Pathol, 2005; 25(1)∶33.
4. 　Oh BK, Lee CH, Park C, et al. Telomerase regulation and progressive telomere shortening of rat hepatic stemlike epithelial cells during in vitro aging [J]. Exp Cell Res, 2004; 298(2)∶445.
5. 　Hatch HM, Zheng D, Jorgensen ML, et al. SDF1alpha/CXCR4: a mechanism for hepatic oval cell activation and bone marrow stem cell recruitment to the injured liver of rats [J]. Cloning Stem Cells, 2002; 4(4)∶339.
6. 　龚加庆，方驰华，李雅. 肝卵圆细胞在进行性肝损伤过程中分布及迁移的实验研究 [J]. 中国普外基础与临床杂志, 2004; 11(5)∶389.
7. 　韦伟，龚建平，裘法祖. 正常和异常肝组织增生中细胞增殖与凋亡的关系 [J]. 第三军医大学学报, 2001； 18（2）∶156.
8. 　Fiegel HC, Gluer S, Roth B, et al. Stemlike cells in human hepatoblastoma [J]. J Histochem Cytochem, 2004; 52(11)∶1495.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on Dynamic Expression of Hepatic Proliferating Cell Nuclear Antigen in The Occurrence and Development of Hepatocellular Carcinoma

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