Significance of Expression of Platelet Derived Growth Factor and Survivin of Hepatocellular Carcinoma with Portal Vein Tumor Thrombosis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Zhefu , CHEN Xiaoping , ZHANG Wanguang

Department of Hepatobiliary Surgery, The Affiliated Qingdao Municipal Hospital, Qingdao Medical College, Qingdao University, Qingdao 266011, China;

Corresponding author：

Keywords：

Hepatocellular carcinoma; Platelet derived growth factor; Survivin gene; Portal vein; Tumor thrombus

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Objective 　To investigate the expressions of platelet derived growth factor (PDGF) and survivin in hepatocellular carcinoma (HCC) with portal vein tumor thrombosis (PVTT) and to evaluate the relationships among the expressions of PDGF, survivin and the proliferation of cancer cells.
Methods 　The expression of PDGF mRNA in 16 cases in HCC with PVTT group was observed by in situ hybridization and the results were compared with that in HCC tissue group. The expressions of PDGF and survivin protein in 36 cases in HCC with PVTT group were detected with immunohistochemistry and it was found that there was a correlation between them. Flow cytometry (FCM) was used to measure the proliferation of cancer cells and it was also used to analyze the relations among PDGF, survivin and the proliferation of cancer cells.
Results 　The expression level of PDGF mRNA in HCC with PVTT group was significantly higher than that in HCC tissue group (P＜0.01). There was a positive correlation between the expressions of PDGF and survivin protein in HCC with PVTT (P＜0.01). The degree of proliferation of cancer cells in PDGF and survivin protein positive group was significantly higher than that in negative group (P＜0.01). Conclusion 　PDGF and survivin gene over-expressed in HCC with PVTT group and there is a positive correlation between the expressions of PDGF and survivin protein. The proliferation of cancer cells increases as the expressions of PDGF and survivin increase.

Citation： LI Zhefu,CHEN Xiaoping,ZHANG Wanguang. Significance of Expression of Platelet Derived Growth Factor and Survivin of Hepatocellular Carcinoma with Portal Vein Tumor Thrombosis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(1): 48-48. doi: Copy

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1. 4　Jin-no K, Tanimizu M, Hyodo I, et al. Circulating platelet-derived endothelial cell growth factor increases in hepatocellular carcinoma patients [Ｊ]. Cancer， 1998; 82(7)∶1260 5　Mise M, Arii S, Higashituji H, et al. Clinical significance of vascular endothelial growth factor and basic fibroblast growth factor gene expression in liver tumor [Ｊ]. Hepatology， 1996; 23(3)∶455.
2. 　左连富，刘洪祥，郭建文，等. 石蜡包埋组织用于流式细胞光度术的单细胞悬液样品制备的研究 [Ｊ]. 临床与实验病理学杂志， 1987; 3(4)∶ 239.
3. 　邓凯贤，钟玲. survivin基因表达与妇科肿瘤关系的研究进展 [Ｊ]. 中华妇产科杂志， 2004; 39(8)∶566.
4. 　Zhou J, Tang ZY, Fan J, et al. Expression of platelet-derived endothelial cell growth factor and vascular endothelial growth factor in hepatocellular carcinoma and portal vein tumor thrombus [Ｊ]. Cancer Res Clin Oncol, 2000; 126(1)∶57.
5. 　Li XM, Tang ZY, Zhou G, et al. Significance of vascular endothelial growth factor mRNA expression in invasion and metastasis of hepatocellular carcinoma [Ｊ]. J Exp Clin Cancer Res， 1998; 17(1)∶13.
6. 　秦军，陈宝琦，刘佃成. 血小板衍生生长因子受体在膀胱癌中的表达意义 [Ｊ]. 第四军医大学学报， 2001; 22(8)∶714.
7. 　张剑，魏先锋，张志远，等. 鼻内镜手术前后鼻腔分泌液中血小板源性生长因子和肿瘤坏死因子α及透明质酸的变化 [Ｊ]. 中华耳鼻喉头颈外科杂志， 2005; 40(7)∶498.
8. 　刘安重，陈孝平，孟春城. 门静脉主支癌栓PDGF mRNA和E-CD蛋白的表达及意义 [Ｊ]. 中华肝胆外科杂志， 2002; 8(7)∶402.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Significance of Expression of Platelet Derived Growth Factor and Survivin of Hepatocellular Carcinoma with Portal Vein Tumor Thrombosis

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