Influence of Genders on Activity of Nuclear Factor-Kappa B in Lungs in Endotoxemic Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

DU Xiaohui ¹ , LI Rong ¹ , XU Yingxin ¹ , YAO Yongming ² , SHEN Chuanan ² , YIN Huinan ²

Department of General Surgery, General Hospital of PLA, Beijing 100853, China;

Corresponding author：

Keywords：

Endotoxemia; Nuclear factor-kappa B; Tumor necrosis factor-α; Lung

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Objective　 To investigate influence of genders on the activity of nuclear factor-kappa B (NF-κB) in lungs of endotoxemic rats.
Methods 　Twenty female and 20 male Wistar rats were randomly divided into four groups as follow: female control group (n=10), male control group (n=10), male endotoxemic group (n=10), and female endotoxemic group (n=10). The endotoxemic rats model was made by injecting lipopolysaccharide (5 mg/kg) into the abdominal cavity. Tissue samples were collected from the lungs in different groups and electrophoresis mobility shift assay was used to measure the activity of NF-κB. The levels of serum TNF-α and estrogen were measured at the same time.
Results 　There was no significant difference between the activities of NF-κB in male and female control groups (1.33±0.24 vs 1.47±0.40）, and there was also no significant difference between other items in these groups as well (P gt;0.05). Yet, the activity of NF-κB (female: 12.10±2.89; male: 19.53±2.12) and the level of TNF-α 〔female: (4.10±0.72) ng/ml; male: (6.37±1.29) ng/ml〕 were significantly increased after injection of lipopolysaccharide (P lt;0.01), and the indices in female group were significantly lower than those in male group (P lt;0.01). Correlation analysis showed that there was a positive relation between the activity of NF-κB in lungs and the level of TNF-α (female: r=0.921 1, P=0.013; male: r=0.907 2, P=0.017), and there was a negative correlation between the activity of NF-κB and the level of estrogen (female: r=-0.887 5, P=0.017; male: r=0.872 3, P=0.022) in both male endotoxemic group and female endotoxemic group (P lt;0.05).
Conclusion 　Gender may be one of the factors that influence the activity of NF-κB in the lungs of endotoxemic rats. While　 on the other hand, endogenous estrogen may protect the lungs of endotoxemic rats from injury by inhibiting the activity of NF-κB.

Citation： DU Xiaohui,LI Rong,XU Yingxin,YAO Yongming,SHEN Chuanan,YIN Huinan. Influence of Genders on Activity of Nuclear Factor-Kappa B in Lungs in Endotoxemic Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(1): 53-55. doi: Copy

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1.
2. 　Abraham E. Nuclear factor-kappa B and its role in sepsis-associated organ failure [J]. J Infect Dis， 2003; 187 Suppl 2∶S364.
3. 　Schroder J, Kahlke V, Staubach KH, et al. Gender differences in human sepsis [J]. Arch Surg， 1998; 133(11)∶1200.
4. 　Yang H, Young DW, Gusovsky F, et al. Cellular events mediated by lipopolysaccharide-stimulated toll-like receptor 4. MD-2 is required for activation of mitogen-activated protein kinases and Elk-1 [J]. J Biol Chem， 2000; 275(27)∶20861.
5. 　Knoferl MW, Diodato MD, Angele MK, et al. Do female sex steroids adversely or beneficially affect the depressed immune responses in males after trauma-hemorrhage? [J]. Arch Surg， 2000; 135(4)∶425.
6. 　Gould IM, MacKenzie FM. The response of Enterobacteriaceae to beta-lactam antibiotics——‘round forms, filaments and the root of all evil’ [J]. J Antimicrob Chemother， 1997; 40(4)∶495.
7. 　Bone RC, Balk RA, Fein AM, et al. A second large controlled clinical study of E5, a monoclonal antibody to endotoxin: results of a prospective, multicenter, randomized, controlled trial. The E5 Sepsis Study Group [J]. Crit Care Med， 1995; 23(6)∶994.
8. 　Hassouna H, Quinn C. Proteolysis of protein C in pooled normal plasma and purified protein C by activated protein C (APC) [J]. Biophys Chem， 2002; 95(2)∶109.
9. 　Mitteregger R, White A, Weber C, et al. In vitro cell culture systems as the basis for an extracorporeal blood purification strategy in multiorgan failure treatment [J]. Ther Apher， 1999; 3(3)∶257.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Influence of Genders on Activity of Nuclear Factor-Kappa B in Lungs in Endotoxemic Rats

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