Role of Apoptosis in Acute Rejection of Pancreaticoduodenal Transplantation in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Tao , LUO Jun , WEN Hao

Department of General Surgery, The First Affiliated Hospital of Xinjiang Medical University, Xinjiang Transplant Institute, Urumqi 830054， China;

Corresponding author：

Keywords：

Pancreas transplantation; Rejection; Apoptotic gene; Duodenum biopsy

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Objective　To investigate the roles of cell apoptosis and the gene expressions of Fas, FasL, bcl-2 and bax in acute rejection of pancreaticoduodenal transplantation and to evaluate the function of duodenum biopsy for early detection of rejection in rats.
Methods　Wistar and SD rats were divided into two groups: ①Wistar rats that underwent allogenic pancreaticoduodenal transplantation from the organs of SD rats; ②Wistar rats that received homogenic transplantation. The grafts were then harvested on day 3, 5 and 7 after the transplantation, and all graft samples were observed with HE staining and TUNEL was also used to detect apoptotic cells. The expressions of Fas, FasL, bcl-2 and bax were measured by immunochemical method. According to Nakhleh’s score, pathologic features of transplanted pancreas and duodenum were ranged from one to three scores in order.
Results　 The percentage of same or different scores between the pathological scores of pancreas and duodenum in allogenic pancreaticoduodenal transplantation group were 61.1% (11/18) and 38.9% (7/18) respectively, and there were 6 specimens of pancreatic tissue got higher scores with only one higher score for duodenum. There were significant differences of histopathologic rejection scores and apoptotic indices between the two groups, respectively (P＜0.05， P＜0.01). Apoptotic indices of pancreas and duodenum both showed positive correlations with histopathologic rejection scores (r=0.965， P＜0.01; r=0.942, P＜0.01). The rejection score and apoptotic index elevated, the expression of FasL increased, bcl-2 decreased, and Fas and bax changed over time after operation.
Conclusion　Apoptosis maybe significantly positive correlated with the degrees of damage of the acute pancreaticoduodenal allograft rejection, and the apoptotic index maybe valuable to estimate the damage. FasL and bcl-2 were significantly related to the impairment of acute pancreatic allograft rejection as well. Duodenum biopsy may contribute to the early diagnosis of the rejecting transplanted tissues.

Citation： LI Tao,LUO Jun,WEN Hao. Role of Apoptosis in Acute Rejection of Pancreaticoduodenal Transplantation in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(1): 59-62. doi: Copy

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1. 　Nakhleh RE, Sutherland DE, Tzardis P, et al. Correlation of rejection of the duodenum with rejection of the pancreas in a pig model of pancreaticoduodenal transplantation [J]. Transplantation， 1993; 56(6)∶1353.
2. 　Matsuno T, Sasaki H, Nakagawa K, et al. Fas antigen expression and apoptosis in kidney allografts [J]. Transplant Proc， 1997; 29(1-2)∶177.
3. 　Kabelitz D. Apoptosis, graft rejection, and transplantation tolerance [J]. Transplantation， 1998; 65(7)∶869.
4. 　Woodle ES, Kulkarni S. Programmed cell death [J]. Transplantation， 1998; 66(6)∶681.
5. 　Kagi D, Vignaux F, Ledermann B, et al. Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity [J]. Science， 1994; 265(5171)∶528.
6. 　Asoh S, Ohta S. Bcl-2 completely blocks Fas-mediated apoptosis in mtDNA-depleted HeLa cells [J]. Biochem Biophys Res Commun， 1997; 237(3)∶659.
7. 　Gaber LW, Stratta RJ, Lo A, et al. Role of surveillance biopsies in monitoring recipients of pancreas alone transplants [J]. Transplant Proc， 2001; 33(1-2)∶1673.
8. 　Zibari GB, Aultman DF, Abreo KD, et al. Roux-en-Y venting jejunostomy in pancreatic transplantation: a novel approach to monitor rejection and prevent anastomotic leak [J]. Clin Transplant， 2000; 14(4 Pt 2)∶380.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Role of Apoptosis in Acute Rejection of Pancreaticoduodenal Transplantation in Rats

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