【Abstract】ObjectiveTo investigate the role of apoptosis-related gene survivin, caspase-3 and cyclin-B1 in gastric carcinoma by detecting the expressions of survivin, caspase-3 and cyclin-B1 in gastric carcinoma.
Methods The expressions of survivin mRNA, caspase-3 mRNA and cyclin-B1 mRNA were determined by reverse transcription-polymerase chain reaction (RT-PCR) method in 30 gastric carcinoma specimens and 10 normal gastric tissue specimens.
ResultsThe positive expression rate of survivin mRNA in 30 gastric carcinoma specimens was 66.7%(20/30). While 10 normal gastric tissue specimens did not express survivin mRNA. Although all of 30 gastric carcinoma tissues and 10 normal gastric tissues expressed caspase-3 mRNA and cyclin-B1 mRNA, the expressions of caspase-3 and cyclin-B1 in 20 survivin-positive gastric carcinoma tissues were significantly lower than those of 10 survivin-negative gastric carcinoma tissues (P＜0.01) and 10 normal gastric tissues (P＜0.01). The expressions of caspase-3 mRNA and cyclinB1 mRNA in 10 survivin-negative gastric carcinoma tissues were significantly lower than those of 10 normal gastric tissues (P＜0.01). And in gastric carcinoma tissues the expression of survivin mRNA was negatively related with that of caspase-3 mRNA （r=-0.923,P＜0.01） and cyclin-B1 mRNA (r=-0.886,P＜0.01), the expression of caspase-3 mRNA was positively related with that of cyclin-B1 mRNA (r=0.892, P＜0.01).
Conclusionsurvivin enhances gastric tumorigenesis, caspase-3 and cyclin-B1 inhibit gastric tumorigenesis.
Citation：
WEN Yayuan,LIU Baohua,HONG Shenglong,FU Tao.. Role of Apoptosis-Related Gene survivin, caspase-3 and cyclin-B1 in Gastric Carcinoma Tumorigenesis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(1): 34-37. doi:
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- 2. Bao R, Connolly DC, Murphy M, et al. Activation of cancerspecific gene expression by the survivin promoter [Ｊ]. J Natl Cancer Inst， 2002; 94(7)∶522.
- 3. Raff M. Cell suicide for beginners [Ｊ]. Nature， 1998; 396(6707)∶119.
- 4. Hu S, Snipas SJ, Vincenz C, et al. Caspase14 is a novel developmentally regulated protease [Ｊ]. J Biol Chem， 1998; 273(45)∶29648.
- 5. Altieri DC. The molecular basis and potential role of survivin in cancer diagnosis and therapy [Ｊ]. Trends Mol Med， 2001; 7(12)∶542.
- 6. Reed JC. Dysregulation of apoptosis in cancer [Ｊ]. J Clin Oncol， 1999; 17(9)∶2941.
- 7. O’Connor DS, Grossman D, Plescia J, et al. Regulation of apoptosis at cell division by p34cdc2 phosphorylation of survivin [Ｊ]. Proc Natl Acad Sci USA， 2000; 97(24)∶13103.
- 8. Suzuki A, Ito T, Kawano H, et al. Survivin initiates procaspase 3/p21 complex formation as a result of interaction with Cdk4 to resist Fasmediated cell death [Ｊ]. Oncogene， 2000; 19(10)∶1346.
- 9. Hoeijmakers JH. Genome maintenance mechanisms for preventing cancer [Ｊ]. Nature， 2001; 411(6835)∶366.
- 10. Dynlacht BD. Regulation of transcription by proteins that control the cell cycle [Ｊ]. Nature， 1997; 389(6647)∶149.
- 11. Muschel RJ, Zhang HB, McKenna WG. Differential effect of ionizing radiation on the expression of cyclin A and cyclin B in HeLa cells [Ｊ]. Cancer Res， 1993; 53(5)∶1128.
- 12. Sayed M, Pelech S, Wong C, et al. Protein kinase CK2 is involved in G2 arrest and apoptosis following spindle damage in epithelial cells [Ｊ]. Oncogene， 2001; 20(48)∶6994.
- 13. Hardcastle IR, Arris CE, Bentley J, et al. N2substituted O6cyclohexylmethylguanine derivatives: potent inhibitors of cyclindependent kinases 1 and 2 [Ｊ]. J Med Chem， 2004; 47(15)∶3710.
- 14. Murakami MS, Moody SA, Daar IO, et al. Morphogenesis during Xenopus gastrulation requires Wee1mediated inhibition of cell proliferation [Ｊ]. Development， 2004; 131(3)∶571.
