Relationship Between Expression of Seprase and Clinicopathologic Characteristics in Colorectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Xuefeng ¹ , JIN Xin ² , WU Guoqiang ¹ , WANG Xize ¹ , LI Jin ¹ , ZONG Xiukun. ¹

Department of General Surgery, General Hospital of Shenyang Army， Shenyang 110016， China;

Corresponding author：

Keywords：

Seprase Colorectal cancer Metastasis Immunohistochemistry

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【Abstract】ObjectiveTo investigate the relationship between expression of Seprase and the clinicopathologic characteristics in colorectal cancer.
MethodsThe expression of Seprase in 50 cases of colorectal cancer was detected with immunohistochemistry, Western-blotting technique and semi-quantitative immunohistochemistry, and the relationship between the expression of Seprase and the clinicopathologic characteristics (age, gender, tumor location, tumor size, gross appearance, depth of infiltration, histological classification, lymph node metastasis, venous infiltration, Dukes stage, distant metastasis) was analyzed.
ResultsThe expression of Seprase was found both in tumor cells and adjacent stromal cells. The level of Seprase protein was higher in cancer tissue than that in normal tissue, and a semiquantitative assessment of the immunohistochemistry revealed a significant correlation between Seprase expression and the Dukes stage and the lymph node metastasis.
ConclusionThe abundant expression of Seprase in colorectal cancer tissue is associated with the Dukes stage and the lymph node metastasis.

Citation： ZHANG Xuefeng,JIN Xin,WU Guoqiang,WANG Xize,LI Jin,ZONG Xiukun.. Relationship Between Expression of Seprase and Clinicopathologic Characteristics in Colorectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(1): 89-93. doi: Copy

1.	Ariga N, Sato E, Ohuchi N, et al. Stromal expression of fibroblast activation protein/seprase, a cell membrane serine proteinase and gelatinase, is associated with longer survival in patients with invasive ductal carcinoma of breast [Ｊ]. Int J Cancer, 2001; 95(1)∶67.
2.	Aoyama A, Chen WT. A 170kDa membranebound protease is associated with the expression of invasiveness by human malignant melanoma cells [Ｊ]. Proc Natl Acad Sci USA， 1990; 87(21)∶8296.
3.	Monsky WL, Lin CY, Aoyama A, et al. A potential marker protease of invasiveness, seprase, is localized on invadopodia of human malignant melanoma cells [Ｊ]. Cancer Res， 1994; 54(21)∶5702.
4.	Papadopoulou S, Scorilas A, Arnogianaki N, et al. Expression of gelatinaseA (MMP2) in human colon cancer and normal colon mucosa [Ｊ]. Tumour Biol， 2001; 22(6)∶383.
5.	Heppner KJ, Matrisian LM, Jensen RA, et al. Expression of most matrix metalloproteinase family members in breast cancer represents a tumorinduced host response [Ｊ]. Am J Pathol， 1996; 149(1)∶273.
6.	Ito A, Nakajima S, Sasaguri Y, et al. Coculture of human breast adenocarcinoma MCF7 cells and human dermal fibroblasts enhances the production of matrix metalloproteinases 1, 2 and 3 in fibroblasts [Ｊ]. Br J Cancer， 1995; 71(5)∶1039.
7.	Scanlan MJ, Raj BK, Calvo B, et al. Molecular cloning of fibroblast activation protein alpha, a member of the serine protease family selectively expressed in stromal fibroblasts of epithelial cancers [Ｊ]. Proc Natl Acad Sci USA， 1994; 91(12)∶5657.
8.	Harvey SR, Sait SN, Xu Y, et al. Demonstration of urokinase expression in cancer cells of colon adenocarcinomas by immunohistochemistry and in situ hybridization [Ｊ]. Am J Pathol, 1999; 155(4)∶1115.
9.	Okada K, Chen WT, Iwasa S, et al. Seprase, a membranetype serine protease, has different expression patterns in intestinal and diffusetype gastric cancer [Ｊ]. Oncology, 2003; 65(4)∶363.
10.	Sato T, Nishimura G, Yonemura Y, et al. Association of immunohistochemical detection of urokinasetype plasminogen activator with metastasis and prognosis in colorectal cancer [Ｊ]. Oncology, 1995; 52(4)∶347.

1. Ariga N, Sato E, Ohuchi N, et al. Stromal expression of fibroblast activation protein/seprase, a cell membrane serine proteinase and gelatinase, is associated with longer survival in patients with invasive ductal carcinoma of breast [Ｊ]. Int J Cancer, 2001; 95(1)∶67.
2. Aoyama A, Chen WT. A 170kDa membranebound protease is associated with the expression of invasiveness by human malignant melanoma cells [Ｊ]. Proc Natl Acad Sci USA， 1990; 87(21)∶8296.
3. Monsky WL, Lin CY, Aoyama A, et al. A potential marker protease of invasiveness, seprase, is localized on invadopodia of human malignant melanoma cells [Ｊ]. Cancer Res， 1994; 54(21)∶5702.
4. Papadopoulou S, Scorilas A, Arnogianaki N, et al. Expression of gelatinaseA (MMP2) in human colon cancer and normal colon mucosa [Ｊ]. Tumour Biol， 2001; 22(6)∶383.
5. Heppner KJ, Matrisian LM, Jensen RA, et al. Expression of most matrix metalloproteinase family members in breast cancer represents a tumorinduced host response [Ｊ]. Am J Pathol， 1996; 149(1)∶273.
6. Ito A, Nakajima S, Sasaguri Y, et al. Coculture of human breast adenocarcinoma MCF7 cells and human dermal fibroblasts enhances the production of matrix metalloproteinases 1, 2 and 3 in fibroblasts [Ｊ]. Br J Cancer， 1995; 71(5)∶1039.
7. Scanlan MJ, Raj BK, Calvo B, et al. Molecular cloning of fibroblast activation protein alpha, a member of the serine protease family selectively expressed in stromal fibroblasts of epithelial cancers [Ｊ]. Proc Natl Acad Sci USA， 1994; 91(12)∶5657.
8. Harvey SR, Sait SN, Xu Y, et al. Demonstration of urokinase expression in cancer cells of colon adenocarcinomas by immunohistochemistry and in situ hybridization [Ｊ]. Am J Pathol, 1999; 155(4)∶1115.
9. Okada K, Chen WT, Iwasa S, et al. Seprase, a membranetype serine protease, has different expression patterns in intestinal and diffusetype gastric cancer [Ｊ]. Oncology, 2003; 65(4)∶363.
10. Sato T, Nishimura G, Yonemura Y, et al. Association of immunohistochemical detection of urokinasetype plasminogen activator with metastasis and prognosis in colorectal cancer [Ｊ]. Oncology, 1995; 52(4)∶347.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Relationship Between Expression of Seprase and Clinicopathologic Characteristics in Colorectal Cancer

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