Change of BAG 3 Expression of Pancreatic cancer Tissues and Cell Lines after Chemotherapy_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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LIAO Quan , ZHAO Yupei , KONG Lingyan , WU Weiwei , CHEN Ge , WU Yuande.

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Keywords：

Pancreatic cancer Chemotherapy BAG- 3

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【Abstract】Objective To analyze the function of BAG3 in antiapoptosis and chemotherapy resistance induction process of pancreatic cancer.Methods The expressions of BAG-3 in pancreatic cancerous tissues of patients with chemotherapy and those without chemotherapy before resection were determined by immunohistochemistry. The expression difference of BAG-3 protein 18 hours after cultured with chemotherapy drugs (concentration of drugs: 5-FU 50 μg/ml, MMC 0.5 μg/ml, EADM 1.5 μg/ml) of 3 pancreatic cancer cell lines （MIACaPa-2, PANC-1, SW1990） was measured through Western blotting method.Results The median positive rate of pancreatic cancer tissue from patients accepted chemotherapy before resection was higher than those not accepted chemotherapy, but there wasn’t significant difference. Eighteen hours after cultured with drugs, the level of BAG-3 of this three cell lines had significant increased compared with control group (P＜0.05).

Conclusion Chemotherapy induces elevation of BAG-3 expression of pancreatic cancer. The upregulate of BAG-3 may associate with the chemotherapy resistance induced by drugs.

Citation： LIAO Quan,ZHAO Yupei,KONG Lingyan,WU Weiwei,CHEN Ge,WU Yuande.. Change of BAG 3 Expression of Pancreatic cancer Tissues and Cell Lines after Chemotherapy. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(6): 591-594. doi: Copy

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1. 1 Brammer R, Buckels J, Bramhall S. Advances in non operative therapy in pancreatic cancer [Ｊ]. Int J Clin Pract， 2000; 54(6)3732 Graber HU, Friess H, Zimmermann A, et al. Bak expression and cell death occur in peritumorous tissue but not in pancreatic cancer cells [Ｊ]. J Gastrointest Surg, 1999; 3(1)743 Korc M. Role of growth factors in pancreatic cancer [Ｊ]. Surg Oncol Clin N Am, 1998; 7(1)254 Liao Q, Ozawa F, Friess H, et al. The antiapoptotic protein BAG3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines [Ｊ]. FEBS Lett, 2001; 503(2-3)1515 Makinen K, Hakala T, Lipponen P, et al. Clinical contribution of bcl2, p53 and Ki67 proteins in pancreatic ductal adenocarcinoma [Ｊ]. Anticancer Res, 1998; 18(1B)6156 Bold RJ, Hess KR, Pearson AS, et al. Prognostic factors in resectable pancreatic cancer: p53 and bcl2 [Ｊ]. J Gastrointest Surg, 1999; 3(3)2637 Ariapart P, BergstedtLindqvist S, van Harmelen V, et al. Resection of pancreatic cancer normalizes the preoperative increase of tumor necrosis factor alpha gene expression [Ｊ]. Pancreatology, 2002; 2(5)4918 Trauzold A, Wermann H, Arlt A, et al. CD95 and TRAIL receptormediated activation of protein kinase C and NFkappaB contributes to apoptosis resistance in ductal pancreatic adenocarcinoma cells [Ｊ]. Oncogene, 2001; 20(31)42589 Takayama S, Xie Z, Reed JC. An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators [Ｊ]. J Biol Chem, 1999; 274(2)78110 廖泉，赵玉沛，蔡力行，等. 胰腺癌细胞对化疗药物敏感性的测定 [Ｊ]. 中华实验外科杂志， 1998； 15（5）44411 Lee JH, Takahashi T, Yasuhara N, et al. Bis, a Bcl2binding protein that synergizes with Bcl2 in preventing cell death [Ｊ]. Oncogene, 1999; 18(46)618312 Doong H, Price J, Kim YS, et al. CAIR1/BAG3 forms an EGFregulated ternary complex with phospholipase Cgamma and Hsp70/Hsc70 [Ｊ]. Oncogene， 2000; 19(38)4385.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Change of BAG 3 Expression of Pancreatic cancer Tissues and Cell Lines after Chemotherapy

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