【Abstract】Objective To investigate the correlation between the expression of fibronectin (FN) in extracellular matrix (ECM) and angiogenesis of gastric carcinoma.Methods The expressions of FN and vascular endothelial growth factor (VEGF) in 20 specimens of normal gastric tissue (normal group) and 80 specimens of gastric carcinoma tissue(gastric carcinoma group) were detected by EnVisonTM immunohistochemical technique. Tumor microvessel densit y (MVD) was evaluated by using antiCD34 antibody as an endothelial marker by the same technique as well. Results The immune complex of FN stained in brown were distributed around glands and in connective tissue of gastric specimens. In normal group, the staining of FN formed intact linear structure at basement membrane and presented regular striae form in connective tissue. In gastric carcinoma group, the integrity of linear structure of FN staining at basement membrane were destroyed to different extent and the staining of FN in connective tissue were changed deeper and distributed irregularly. The expression of VEGF and the value of MVD in the gastric carcinoma group was higher than those in normal group’s(P＜0.01, P＜0.01, respectively).This study indicated, that in gastric carcinoma group, the degree of FN expression in connective tissue had statistically positive correlations with the degree of VEGF expression and MVD value(P＜0.01, P＜0.05, respectively). Whereas the destruction extent of linear structure of FN staining at basement membrane showed no correlation with VEGF expression and MVD value(P gt;0.05, P gt;0.05, respectively).
Conclusion The higher expression of FN in connective tissue of gastric carcinoma may well play a critical role in its process of angiogenesis and vasculogenesis. There may be an cooperative interactions between FN and VEGF in the process of angiogenesis and vasculogenesis of gastric carcinoma.
Citation：
JIAO Zuoyi,LIU Bo,LI Jin,WANG Zhengxia,XU Mingqing,YANG Jiayin,WANG Wentao,YAN Lnan.. Study on Correlation Between Expression of Fibronectin in Extracellular Matrix and Angiogenesis of Gastric Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(6): 605-608下转617. doi:
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曾益新. 肿瘤学 [M]. 第2版. 北京: 人民卫生出版社, 2003250~2582 谭曾鲁, 周柔丽. 医学细胞生物学 [M]. 第1版. 北京: 北京医科大学出版社, 1992100~1033 Wijelath ES, Murray J, Rahman S, et al. Novel vascular endothelial growth factor binding domains of fibronectin enhance vascular endothelial growth factor biological activity [Ｊ]. Circ Res, 2002; 91(1)254 Havenith MG, Arends JW, Simon R, et al. Type IV collagen immunoreactivity in colorectal cancer. Prognostic value of basement membrane deposition [Ｊ]. Cancer, 1988; 62(10)22075 张健康, 李晓丽, 李亮成, 等. Ⅳ型胶原, 层粘连蛋白, 纤维粘连蛋白在胃癌中表达的意义 [Ｊ]. 山西医科大学学报, 2000; 31(2)1146 Weidner N. Current pathologic methods for measuring intratumoral microvessel density within breast carcinoma and other solid tumors [Ｊ]. Breast Cancer Res Treat, 1995; 36(2)1697 Feng CW, Wang LD, Jiao LH, et al. Expression of p53, inducible nitric oxide synthase and vascular endothelial growth factor in gastric precancerous and cancerous lesions: correlation with clinical features [Ｊ]. BMC Cancer, 2002; 2(1)88 刘秉文, 陈俊杰. 医学分子生物学 [M]. 第1版. 北京: 中国协和医科大学出版社, 200088~929 Fujita J. Correlation between laminin and fibronectin on the basement membrane and tumor progression in early gastric cancer: an immunohistochemical study [Ｊ]. Hokkaido Igaku Zasshi, 2000; 75(1)25.
