【Abstract】ObjectiveTo inquire into the regulative effect of interferon (IFN) on human leucocyte antigen-DR(HLADR) expression in human colonic cancer cells.
MethodsThe expression of HLA-DR in 4 colonic cancer cell lines HCT-8，lovo，SW-480 and Ls-174-T was detected with immunohistochemical SP and ELISA method before and after being stimulated by different doses of α-IFN or γ-IFN.
ResultsAll cell lines except lovo cell were HLA-DR positive before stimulation with α-IFN or γ-IFN. HLA-DR expression was enhanced on all of the 4 cell lines after stimulation, and it was correlated with γ-IFN and the dose of γ-IFN. The effect of γ-IFN was more obvious than that of α-IFN.
ConclusionIFN can enhance HLA-DR expression in human colonci cancer cells and clinical therapy of IFN for colon cancer has a certain applying prospect.
Citation：
HUANG Wenguang,LIU Gang,FANG Quan,HUANG Hanju.. Effect of Interferon on Human Leucocyte Antigen-DR Expression in Human Colonic Cancer Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(1): 15-18. doi:
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- 1. Mayer L, Eisenhardt D, Salomon P, et al. Expression of class II molecules on intestinal epithelial cells in humans. Differences between normal and inflammatory bowel disease [Ｊ]. Gastroenterology, 1991; 100(1)∶3.
- 2. Real FX, Carrato A, Schuessler MH, et al. IFNgammaregulated expression of a differentiation antigen of human cells [Ｊ]. J Immunol, 1988; 140(5)∶1571.
- 3. Lazaris AC, Theodoropoulos GE, Davaris PS, et al. Heat shock protein 70 and HLADR molecules tissue expression. Prognostic implications in colorectal cancer [Ｊ]. Dis Colon Rectum, 1995; 38(7)∶739.
- 4. Redondo M, RuizCabello F, Concha A, et al. Altered HLA class I expression in nonsmall cell lung cancer is independent of cmyc activation [Ｊ]. Cancer Res, 1991; 51(9)∶2463.
- 5. Gastl G, Marth C, Leiter E, et al. Effects of human recombinant alpha 2 arginterferon and gammainterferon on human breast cancer cell lines: dissociation of antiproliferative activity and induction of HLADR antigen expression [Ｊ]. Cancer Res, 1985; 45(7)∶2957.
- 6. Yang LJ, Sui YF, Chen ZN. Enhancement of HLADR expression in human hepatocellular carcinoma with interferon [Ｊ]. Chin J Cancer Res, 1999; 11(4)∶241.
- 7. Toes RE, Ossendorp F, Offringa R, et al. CD4 T cells and their role in antitumor immune responses [Ｊ]. J Exp Med, 1999; 189(5)∶753.
