Protective Effect of Melatonin on Renal Injury Induced by Obstructive Jaundice in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Xiaoming ¹ , KOU Zhiming ¹ , KANG Erhu ² , ZHANG Youcheng. ¹

Department of General Surgery, The Second Hospital, Lanzhou University, Lanzhou 730030, China;

Corresponding author：

Keywords：

Obstructive jaundice Kidney disfunction Nitric oxide Inducible nitric oxide synthase Melatonin

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【Abstract】ObjectiveTo investigate the protective effect of melatonin on renal injury induced by bile duct ligation in rats.
MethodsSixtyfour rats were randomly divided into four experimental groups (n=16 rats per group): the control group (CN), sham operative group (SO), bile duct ligation group (BDL) and bile duct ligation melatonin treatment group (BDL+Mel). Obstructive jaundice was induced by common bile duct ligation. Plasma level of nitric oxide (NO), total bilirubin (TB), direct bilirubin (DB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea nitrogen (BUN) and creatinine (Cr) were measured 4 d and 8 d after operation. NO and inducible nitric oxide synthase (iNOS) in renal tissue were detected at the same time point, too. Histopathological changes of kidneys were examined by HE staining.
ResultsIn BDL group, the plasma levels of NO, TB, DB, ALT, AST, BUN and Cr were higher than those of SO group (P＜0.01）, and the level of NO and activities of iNOS in renal tissue were significantly increased (P＜0.01）. However, in BDL+Mel group, the plasma levels of NO, ALT, AST, BUN and Cr were lower than those of the BDL group (P＜0.01）, and the level of NO and activities of iNOS in renal tissue were significantly suppressed (P＜0.01）; histopathological changes of kidneys were improved.
ConclusionAugmentation of iNOS activities and increasing of NO production in local tissue contributed to renal injury induced by bile duct ligation, and the mode of melatonin’s protective actions, at least in part, relates to interference with no pathways.

Citation： ZHANG Xiaoming,KOU Zhiming,KANG Erhu,ZHANG Youcheng.. Protective Effect of Melatonin on Renal Injury Induced by Obstructive Jaundice in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(1): 55-58. doi: Copy

1.	GonzalezCorrea JA, De La Cruz JP, MartinAurioles E, et al. Effects of SadenosylLmethionine on hepatic and renal oxidative stress in an experimental model of acute biliary obstruction in rats [Ｊ]. Hepatology, 1997; 26(1)∶121.
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4.	Crespo E, Macias M, Pozo D, et al. Melatonin inhibits expression of the inducible NO synthase II in liver and lung and prevents endotoxemia in lipopolysaccharideinduced multiple organ dysfunction syndrome in rats [Ｊ]. FASEB J, 1999; 13(12)∶1537.
5.	Dugo L, Serraino I, Fulia F, et al. Effect of melatonin on cellular energy depletion mediated by peroxynitrite and poly (ADPribose) synthetase activation in an acute model of inflammation [Ｊ]. J Pineal Res, 2001; 31(1)∶76.
6.	Nath KA, Norby SM. Reactive oxygen species and acute renal failure [Ｊ].Am J Med， 2000; 109(8)∶ 665.
7.	Kannan K, Jain SK. Oxidative stress and apoptosis [Ｊ].Pathophysiology， 2000; 7(3)∶153.
8.	Jaeschke H. Reactive oxygen and mechanisms of inflammatory liver injury [Ｊ].J Gastroenterol Hepatol， 2000; 15(7)∶718.
9.	Ohta Y, Kongo M, Kishikawa T. Melatonin exerts a therapeutic effect on cholestatic liver injury in rats with bile duct ligation [Ｊ]. J Pineal Res, 2003; 34(2)∶119.
10.	Cruz A, Padillo FJ, Tunez I, et al. Melatonin protects against renal oxidative stress after obstructive jaundice in rats [Ｊ]. Eur J Pharmacol, 2001; 425(2)∶135.
11.	Chen CY, Shiesh SC, Tsao HC, et al. Protective effect of melatonin on renal injury of rats induced by bile duct ligation [Ｊ].Dig Dis Sci, 2001; 46(4)∶927.

1. GonzalezCorrea JA, De La Cruz JP, MartinAurioles E, et al. Effects of SadenosylLmethionine on hepatic and renal oxidative stress in an experimental model of acute biliary obstruction in rats [Ｊ]. Hepatology, 1997; 26(1)∶121.
2. 刘祥德，李卫，何振平，等. NO与梗阻性黄疸时肾损害的关系 [Ｊ]. 第二军医大学学报， 2001； 23（7）∶795.
3. Gilad E, Wong HR, Zingarelli B, et al. Melatonin inhibits expression of the inducible isoform of nitric oxide synthase in murine macrophages: role of inhibition of NFkappaB activation [Ｊ]. FASEB J, 1998; 12(9)∶685.
4. Crespo E, Macias M, Pozo D, et al. Melatonin inhibits expression of the inducible NO synthase II in liver and lung and prevents endotoxemia in lipopolysaccharideinduced multiple organ dysfunction syndrome in rats [Ｊ]. FASEB J, 1999; 13(12)∶1537.
5. Dugo L, Serraino I, Fulia F, et al. Effect of melatonin on cellular energy depletion mediated by peroxynitrite and poly (ADPribose) synthetase activation in an acute model of inflammation [Ｊ]. J Pineal Res, 2001; 31(1)∶76.
6. Nath KA, Norby SM. Reactive oxygen species and acute renal failure [Ｊ].Am J Med， 2000; 109(8)∶ 665.
7. Kannan K, Jain SK. Oxidative stress and apoptosis [Ｊ].Pathophysiology， 2000; 7(3)∶153.
8. Jaeschke H. Reactive oxygen and mechanisms of inflammatory liver injury [Ｊ].J Gastroenterol Hepatol， 2000; 15(7)∶718.
9. Ohta Y, Kongo M, Kishikawa T. Melatonin exerts a therapeutic effect on cholestatic liver injury in rats with bile duct ligation [Ｊ]. J Pineal Res, 2003; 34(2)∶119.
10. Cruz A, Padillo FJ, Tunez I, et al. Melatonin protects against renal oxidative stress after obstructive jaundice in rats [Ｊ]. Eur J Pharmacol, 2001; 425(2)∶135.
11. Chen CY, Shiesh SC, Tsao HC, et al. Protective effect of melatonin on renal injury of rats induced by bile duct ligation [Ｊ].Dig Dis Sci, 2001; 46(4)∶927.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Protective Effect of Melatonin on Renal Injury Induced by Obstructive Jaundice in Rats

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