Expression of Tumor Necrosis Factor and Its Receptor in Gallstone and Gallbladder Carcinoma Tissue_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SHI Jingsen , ZHOU Liansuo , ZHU Aijun , YANG Yijun

Lab of Hepatobiliary Surgery, The First Hospital of Xi’an Jiaotong University, Xi’an 710061, China;

Corresponding author：

Keywords：

Gallstone; Gallbladder carcinoma; Tumor necrosis factor; Immunohistochemistry; In situ hybridization

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Objective To explore the expression of tumor necrosis factor (TNF) mRNA, TNF and TNFR in the gallbladder mucosa which developed from hyperplasia, dysplasia to carcinoma, and to further discuss the relationship between TNF and pathogenesis of gallbladder carcinoma.
Methods In situ hybridization and immunohistochemistry were used to determine TNF mRNA, TNF protein and TNFR protein expression in hyperplasia, dysplasia and carcinoma of gallbladder.
Results ①No one of 20 cases of gallbladder hyperplasia was found to express TNF mRNA, while 4 of 20 (20%) cases of dysplasia and 18 of 20 (90%) cases of carcinoma were found to express TNF mRNA (P＜0.05). ②For the expression of TNF mRNA in mononuclear cells (MNC), positive staining was found in 15% of gallbladder hyperplasia, 85% of dysplasia and 90% of carcinoma, respectively (P＜0.05). The cell numbers of positive staining MNC were 4.85±1.50, 6.00±2.71 and 9.33±3.07, respectively (P＜0.05). ③In gallbladder carcinoma, the cell number of carcinoma and MNC with positive TNF mRNA expression was correlated with clinical stage (P＜0.05). The higher the clinical stage, the more the positive staining cell numbers. The positive staining cell numbers of carcinoma in stage Ⅰ-Ⅲ and Ⅳ-Ⅴ were 9.13±4.39 and 14.80±4.02, respectively (P＜0.01), and the positive staining cell numbers of MNC were 7.13±2.53 and 11.10±2.23, respectively (P＜0.05). ④The cell numbers of carcinoma and MNC with TNF mRNA expression increased with tumor size. In tumors with diameter over 2 cm and less than 2 cm, the positive staining cell numbers of carcinoma were 14.00±4.20 and 8.83±4.96, respectively (P＜0.05), and that of MNC were 10.50±2.54 and 7.00±2.83, respectively (P＜0.05). ⑤The region of TNF protein expression was similar to that of TNF mRNA, but TNF protein expression was more frequent and wider than that of TNF mRNA. ⑥The tumor necrosis factor receptor was expressed in tumoral vascular endothelial cells and MNC in all cases of carcinoma, but was negatively stained in mucosa epithelial cells and tumor cells of all cases. ⑦There was positive linear correlation in TNF mRNA between tumor cell and MNC (r=0.687, P＜0.01), same as that in TNF protein expression (r=0.742, P＜0.01); and there was positive linear correlation in tumor cell between TNF mRNA and TNF protein expression (r=0.847, P＜0.01), same as that in MNC (r=0.643, P＜0.01).
Conclusion The TNF mRNA and TNF protein expression are increasing during the development of gallbladder mucosa epithelial from hyperplasia, dysplasia to carcinoma, and increasing with tumor stage. It suggests that TNF may contribute to carcinogenesis of gallbladder carcinoma induced by gallstone, and related to the progression of gallbladder carcinoma.

Citation： SHI Jingsen,ZHOU Liansuo,ZHU Aijun,YANG Yijun. Expression of Tumor Necrosis Factor and Its Receptor in Gallstone and Gallbladder Carcinoma Tissue. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(1): 40-44. doi: Copy

1.	Miles DW, Happerfield LC, Naylor MS, et al. Expression of tumour necrosis factor (TNF alpha) and its receptors in benign and malignant breast tissue [Ｊ]. Int J Cancer, 1994; 56(6)∶ 777.
2.	Izutani P, Katoh M, Asano S, et al. Enhanced expression of manganese superoxide mRNA and increased TNFalpha mRNA expression by gastric mucosa in gastric cancer [Ｊ]. World J Surg, 1996; 20(2)∶228.
3.	石景森, 王炳煌主编. 外科黄疸疾病诊断治疗学 [M]. 北京: 人民军医出版社, 2003∶364~380.
4.	Sheen chen SM, Chen WJ, Eng HL, et al. Serum concentration of tumor necrosis factor in patients with breast cancer [Ｊ]. Breast Cancer Res Treat, 1997; 43(3)∶211.
5.	胡铭荣, 徐乃元, 陈易人. 内源性肿瘤坏死因子与直肠癌关系的探讨 [Ｊ]. 实用肿瘤杂志, 1997； 12(4)∶160.
6.	石景森, 周连锁, 焦兴元, 等. 胆囊癌与胆结石病人血清、胆汁中肿瘤坏死因子及其受体检测的临床意义 [Ｊ]. 中华肝胆外科杂志, 2001； 7(2):82.
7.	Feldmann M, Roiff I, Brostoff J, et al. Immunology [M]. Third edition. London: Mosby, 1994∶77-84.
8.	Wu S, Boyer CM, Whitaker RS, et al. Tumor necrosis factor alpha as an autocrine and paracrine growth factor for ovarian cancer: monokine induction of tumor cell proliferation and tumor necrosis factor alpha expression [Ｊ]. Cancer Res, 1993； 53(8)∶1939.
9.	田伯乐, 张肇达, 付岚, 等. 急性坏死性胰腺炎血清TNF活性与胰组织损害 [Ｊ]. 中国普外基础与临床杂志, 1999； 6(1)∶13.
10.	李云, 钱家勤, 秦仁义, 等. 急性胰腺炎患者外周血IL6和sTNFR的变化及其临床意义 [Ｊ]. 中国普外基础与临床杂志, 2000； 7(5)∶313.

1. Miles DW, Happerfield LC, Naylor MS, et al. Expression of tumour necrosis factor (TNF alpha) and its receptors in benign and malignant breast tissue [Ｊ]. Int J Cancer, 1994; 56(6)∶ 777.
2. Izutani P, Katoh M, Asano S, et al. Enhanced expression of manganese superoxide mRNA and increased TNFalpha mRNA expression by gastric mucosa in gastric cancer [Ｊ]. World J Surg, 1996; 20(2)∶228.
3. 石景森, 王炳煌主编. 外科黄疸疾病诊断治疗学 [M]. 北京: 人民军医出版社, 2003∶364~380.
4. Sheen chen SM, Chen WJ, Eng HL, et al. Serum concentration of tumor necrosis factor in patients with breast cancer [Ｊ]. Breast Cancer Res Treat, 1997; 43(3)∶211.
5. 胡铭荣, 徐乃元, 陈易人. 内源性肿瘤坏死因子与直肠癌关系的探讨 [Ｊ]. 实用肿瘤杂志, 1997； 12(4)∶160.
6. 石景森, 周连锁, 焦兴元, 等. 胆囊癌与胆结石病人血清、胆汁中肿瘤坏死因子及其受体检测的临床意义 [Ｊ]. 中华肝胆外科杂志, 2001； 7(2):82.
7. Feldmann M, Roiff I, Brostoff J, et al. Immunology [M]. Third edition. London: Mosby, 1994∶77-84.
8. Wu S, Boyer CM, Whitaker RS, et al. Tumor necrosis factor alpha as an autocrine and paracrine growth factor for ovarian cancer: monokine induction of tumor cell proliferation and tumor necrosis factor alpha expression [Ｊ]. Cancer Res, 1993； 53(8)∶1939.
9. 田伯乐, 张肇达, 付岚, 等. 急性坏死性胰腺炎血清TNF活性与胰组织损害 [Ｊ]. 中国普外基础与临床杂志, 1999； 6(1)∶13.
10. 李云, 钱家勤, 秦仁义, 等. 急性胰腺炎患者外周血IL6和sTNFR的变化及其临床意义 [Ｊ]. 中国普外基础与临床杂志, 2000； 7(5)∶313.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of Tumor Necrosis Factor and Its Receptor in Gallstone and Gallbladder Carcinoma Tissue

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