Relationship Between Tumor Angiogenesis and Expression of Vascular Endothelial Growth Factor and Nitric Oxide Synthase in Human Gastric Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Guangyi , WANG Xu

Department of General Surgery, The First Hospital of Jilin University, Changchun 130021, China;

Corresponding author：

Keywords：

Vascular endothelial growth factor; Nitric oxide synthase; Gastric cancer; Tumor angiogenesis; Microvessel density

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Abstract Full text Figures/Tables Video References Cited by

Objective To study the expression of inducible nitric oxide synthase (iNOS)，endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) in human gastric cancer and their relationship with tumor angiogenesis and to investigate the interaction of NOS and VEGF in gastric cancer.
Methods The expression and distribution of VEGF, iNOS and eNOS in 34 gastric cancer specimens were detected with immunohistochemistry. Microvessel density (MVD) was counted with FⅧRAg immune specific staining.
Results The expression rates of iNOS, eNOS and VEGF in 34 gastric cancers were 73.5%, 82.4% and 91.2% respectively. The expression of VEGF had a significant positive relation with iNOS, but not with eNOS. The MVDs of VEGF or iNOS positive gastric cancers were obviously higher than those of VEGF or iNOS negative gastric cancers. There was no significant difference between the MVDs of eNOS positive gastric cancers and eNOS negative ones.
Conclusion MVD increases with increase of expression of VEGF and iNOS in gastric cancer. It is indicated that VEGF and iNOS can promote gastric cancer angiogenesis. VEGF and iNOS have a significant positive correlation, which suggests that in human gastric cancer, iNOS plays an important role in the production and action of VEGF.

Citation： WANG Guangyi,WANG Xu. Relationship Between Tumor Angiogenesis and Expression of Vascular Endothelial Growth Factor and Nitric Oxide Synthase in Human Gastric Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(1): 55-57. doi: Copy

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1. McMahon G. VEGF receptor signaling in tumor angiogenesis[J]. Oncologist, 2000； 5 Suppl 1∶ 3.
2. Ziche M, Morbidelli E, Masini S, et al. Nitric oxide mediates angiogenesis in vivo and endothelial cell growth and migration in vitro promoted by substance P [J]. J Clin Invest, 1994； 94(5)∶ 2036.
3. Mattern J, Koomagi R, Volm M. Coexpression of VEGF and bFGF in human epidermoid lung carcinoma is associated with increased vessel density [J]. Anticancer Res, 1997； 17(3C)∶ 2249.
4. Vermeulen PB, Gasparini G, Fox SB, et al. Quantification of angiogenesis in solid human tumors: an international consensus on the methodology and criteria of evaluation [J]. Eur J Cancer, 1996； 32(14)∶ 2474.
5. 杨毅军，石景森. VEGF与肿瘤血管生成 [J]. 中国普外基础与临床杂志, 2001； 8(3)∶205.
6. Moochhala S, Rajnakova A. Role of nitric oxide in cancer biology [J]. Free Radic Res, 1999； 31(6)∶ 671.
7. Xie K, Fidler IJ. Therapy of cancer metastasis by activation of the inducible nitric oxide synthase [J]. Cancer Metastasis Rev, 1998； 17(1)∶ 55.
8. Thomsen LL, Miles DW. Role of nitric oxide in tumour progression: lessons from human tumours [J]. Cancer Metastasis Rev, 1998； 17(1)∶ 107.
9. Jenkins DC, Charles IG, Thomsen LL, et al. Roles of nitric oxide in tumor growth [J]. Proc Natl Acad Sci USA, 1995； 92(10)∶ 4392.
10. Lala PK, Orucevic A. Role of nitric oxide in tumor progression: lessons from experimental tumors [J]. Cancer Metastasis Rev, 1998; 17(1)∶91.
11. Jozkowicz A, Pankiewicz J, Dulak J, et al. Nitric oxide mediates the mitogenic effects of insulin and vascular endothelial growth factor but not of leptin in endothelial cells [J]. Acta Biochim Pol, 1999; 46(3)∶703.
12. Michel JB. Role of endothelial nitric oxide in the regulation of the vasomotor system [J]. Pathol Biol (Paris), 1998; 46(3)∶ 181.
13. Konopka TE, Barker JE, Bamford TL, et al. Nitric oxide synthase Ⅱ gene disruption: implicatons for tumor growth and vascular endothelial growth factor production [J]. Cancer Res, 2001; 61(7)∶ 3182.
14. Kimura H, Weisz A, Kurashima Y, et al. Hypoxia response element of the human vascular endothelial growth factor gene mediates transcriptional regulation by nitric oxide: control of hypoxiainducible factor1 activity by nitric oxide [J]. Blood， 2000; 95(1)∶ 189.
15. Chavakis E, Dernbach E, Hermann C, et al. Oxidized LDL inhibits vascular eneothelial growth factorinduced endothelial cell migration by an inhibitory effect on the Akt/endothelial nitric oxied synthase pathway [J]. Circulation, 2001; 103(16)∶ 2102.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Relationship Between Tumor Angiogenesis and Expression of Vascular Endothelial Growth Factor and Nitric Oxide Synthase in Human Gastric Cancer

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