Expression of CK20 mRNA in Peripheral Blood of Patients with Colorectal Cancer and Its Clinical Significance_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SUN Jianwei ¹ , HUA Yingkun ² , JIA Ling ¹ , LIN Renlong ¹ , YANG Kunxian ¹ , CHEN Jumin ¹

Department of Tumor, The First People’s Hospital of Yunnan Province， Kunming 650032, China;

Corresponding author：

Keywords：

Colorectal carcinoma; CK20 mRNA protein; Peripheral blood

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Objective To detect expression of CK20 mRNA in peripheral blood of patients with colorectal carcinoma and its clinical significance.
Methods Using the reverse transcriptase-polymerase chain reaction (RT-PCR),CK20 mRNA expression was examined in peripheral blood from 42 patients with colorectal carcinoma before and after operation, 20 healthy volunteers, 20 fresh colorectal carcinoma samples.
Results The positive expression rates of CK20 mRNA were 45.24%(19/42) and 33.33%(14/42) before and after operation in 42 colorectal carcinoma patients respectively. All 20 fresh colorectal carcinoma samples revealed expression of CK20 mRNA, but the 20 normal blood samples did not.
Conclusion The detection of CK20 mRNA in peripheral blood is helpful to early diagnose, assess the prognosis and make a correct treatment of colorectal carcinoma.

Citation： SUN Jianwei,HUA Yingkun,JIA Ling,LIN Renlong,YANG Kunxian,CHEN Jumin. Expression of CK20 mRNA in Peripheral Blood of Patients with Colorectal Cancer and Its Clinical Significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(2): 119-121. doi: Copy

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1. Burchill SA, Bradbury MF, Pittman K, et al. Detection of epithelial cancer cells in peripheral blood by reverse transcriptasepolymerase chain reaction [J]. Br J Cancer, 1995; 71(2)∶278.
2. Bustin SA, Gyselman VG, Williams NS, et al. Detection of cytokeratins 19/20 and guanylyl cyclase C in peripheral blood of colorectal cancer patients [Ｊ]. Br J Cancer, 1999; 79(11-12)∶1813.
3. Peck K, Sher YP, Shih JY, et al. Detection and quantitation of circulating cancer cells in the peripheral blood of lung cancer patients [Ｊ]. Cancer Res, 1998; 58(13)∶2761.
4. Aihara T, Noguchi S, Ishikawa O, et al. Detection of pancreatic and gastric cancer cells in peripheral and portal blood by amplification of keratin 19 mRNA with reverse transcriptasepolymerase chain reaction [Ｊ]. Int J Cancer, 1997; 72(3)∶408.
5. Datta YH, Adams PT, Drobyski WR, et al. Sensitive detection of occult breast cancer by the reversetranscriptase polymerase chain reaction [Ｊ]. J Clin Oncol, 1994; 12(3)∶475.
6. Heiss MM, Allgayer H, Gruetzner KU, et al. Individual development and uPAreceptor expression of disseminated tumour cells in bone marrow: a reference to early systemic disease in solid cancer [Ｊ]. Nat Med, 1995; 1(10)∶1035.
7. Gala JL, Loric S. Sensitivity of specificity of reverse transcriptasepolymerase chain reaction assays: the real challenge for molecular staging of prostatic carcinomas [Ｊ]. Int J Cancer, 1998; 77(1)∶161.
8. Wolfgang H, Stefan A, Loening. Detection of illegitimate transcripts of prostatespecific antigen mRNA in blood by reverse transcriptionpolymerase chain reaction [Ｊ]. Int J cancer, 1998; 77(1)∶164.
9. 骆成玉，李世拥. 大肠癌患者外周血癌细胞检测临床意义 [Ｊ]. 中华实验外科杂志, 1999; 16（3）∶19.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of CK20 mRNA in Peripheral Blood of Patients with Colorectal Cancer and Its Clinical Significance

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