Objective To evaluate the expression of cyclin E and p27kip1 protein and their significance in gallbladder carcinoma.
Methods SP immunohistochemistry was used to detect the expression of cyclin E and p27kip1 protein in 41 cases gallbladder carcinomas,15 cases chronic cholecystitis tissues.
Results The positive rate of cyclin E in gallbladder carcinoma was 61.0%（25/41）,which was significantly higher than that in chronic cholecystitis (20.0%，3/15)，P＜0.05; The expression of cyclin E positively correlated with tumor TNM staging (r=0.314,P＜0.05). The positive rate of p27kip1 in gallbladder carcinoma was 53.7%(22/41),which was lower than that in chronic cholecystitis (100%). The positive rate of p27kip1 was decreased with the poor differentiation and progression of TNM staging. There was negative correlation between cyclin E and p27kip1 expression (r=-0.342,P＜0.05).
Conclusion The high expression of cyclin E and the decreased expression of p27kip1 result in abnomal regulation of cell cycle,which may be associated with gallbladder carcinogenesis and progression.
Citation：
ZHOU Yanming,LI Yumin,ZHU Youquan,WANG Xiaoqin,ZHAO Datong,ZENG Feng. Expression and Significance of Cyclin E and p27kip1 in Gallbladder Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(2): 148-150. doi:
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- 1. 夏加增， 杨继昌， 张熔熔， 等. 胃癌中cyclin D1、cyclin E和p27的表达及其意义 [J].中国普外基础与临床杂志， 2001； 8（3）∶148.
- 2. 李远晃， 徐志林. 胃癌组织cyclin E和p21WAF1/CIP1蛋白表达的意义 [J].临床与实验病理学杂志， 2001； 17（5）∶419.
- 3. Polyak K,Lee MH,ErdjumentBromage H,et al.Cloning of p27Kip1, a cyclindependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals [J].Cell, 1994； 78(1)∶59.
- 4. Kawamata N, Morosetti R, Miller CW, et al. Molecular analysis of the cyclindependent kinase inhibitor gene p27/kip1 in human malignancies [J]. Cancer Res, 1995； 55(11)∶2266.
- 5. Tan P, Cady B, Wanner M, et al. The cell cycle inhibitor p27 is an independent prognostic marker in small(T1a,b) invasive breast carcinomas [J]. Cancer Res, 1997； 57(7)∶1259.
- 6. Yasui W, Kudo Y, Semba S, et al.Reduced expression of cyclindependent kinase inhibitor p27kip1 is associated with advanced stage and invasiveness of gastric carcinomas [J]. Jpn J Cancer Res, 1997； 88(7)∶625.
- 7. 郭贵龙， 姚榛祥. p27在甲状腺滤泡状肿瘤中的表达及其意义 [J]. 中国普外基础与临床杂志， 2001； 8（5）∶324.
- 8. 周奇， 王广田， 梁力建， 等. p27在肝细胞癌中的表达及其意义 [J]. 中国普外基础与临床杂志， 2003； 10（2）∶134.
