ObjectiveTo develop and modify a rat model of partial liver transplantation with hepatic arterialization and to investigate the postoperative histopathologic changes of the grafts. MethodsModified twocuff technique was applied to establish a rat model of partial liver transplantation, and the donor’s celiac artery was anastomosed with the recipient’s right renal artery. The biopsy of the grafts was performed on the day 1,2,4 and 7 after transplantation. Results85.4% of the recipients had survived for one week.Nuclear mitoses were seen on the postoperative day 2 by microscopy, while on the day 4 they were found occasionally. Two nucleus cells were seen on the day 4 and 7. Conclusion The stability of the model is improved by modifying the technique. Liver acupuncture biopsy is a simple, safe and feasible way to be applied in the research of postoperative problems of liver transplantation. Reconstruction of hepatic artery is possible to enhance the regeneration of liver grafts.
Citation：
HUANG Xinli,ZHANG Feng,WANG Xuehao,CHENG Feng,LU Sen,QIAN Xiaofeng,XIA Yongxiang. Research of Partial Liver Transplantation with Hepatic Arterialization in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(3): 224-227. doi:
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Lee S, Charters AC, Chandler JG, et al. A technique for orthotopic liver transplantation in the rat [Ｊ]. Transplantation, 1973; 16(6)∶664.
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Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ]. Transplantation, 1979; 28(1)∶47.
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Gao W, Lemasters JJ, Thurman RG. Development of a new method for hepatic rearterialization in rat orthotopic liver transplantation. Reduction of liver injury and improvement of surgical outcome by arterialization [Ｊ]. Transplantation, 1993; 56(1)∶19.
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Reck T, Steinbauer F, Steinbauer M, et al. Impact of arterialization on hepatic oxygen supply, tissue energy phosphates, and outcome after liver transplantation in the rat [Ｊ]. Transplantation, 1996; 62(5)∶582.
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Howden B, Jablonski P, Grossman H, et al. The importance of the hepatic artery in rat liver transplantation [Ｊ]. Transplantation, 1989; 47(3)∶428.
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Omura T, Ascher NL, Emond JC. Fiftypercent partial liver transplantation in the rat [Ｊ]. Transplantation, 1996; 62(2)∶292.
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钱晓峰， 王学浩， 张峰， 等. 大鼠减体积肝移植模型的技术改进及肝再生的初步观察 [Ｊ]. 南京医科大学学报， 2003； 23（3）∶221.
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孙鹏，蔡端， 张群华，等. 非协调性异种肝移植动物模型的建立： 动脉化模型与静脉化模型的比较 [Ｊ]. 中华肝胆外科杂志， 2001； 7（7）∶442.
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Li J, Dahmen U, Dirsch O, et al. Modified sleeve anastomosis for reconstruction of the hepatic artery in rat liver transplantation [Ｊ]. Microsurgery, 2002; 22(2)∶62.
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| 10. |
Knoop M, Bachmann S, Keck H, et al. Experience with cuff rearterialization in 600 orthotopic liver grafts in the rat [Ｊ]. Am J Surg, 1994; 167(3)∶360.
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- 1. Lee S, Charters AC, Chandler JG, et al. A technique for orthotopic liver transplantation in the rat [Ｊ]. Transplantation, 1973; 16(6)∶664.
- 2. Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ]. Transplantation, 1979; 28(1)∶47.
- 3. Gao W, Lemasters JJ, Thurman RG. Development of a new method for hepatic rearterialization in rat orthotopic liver transplantation. Reduction of liver injury and improvement of surgical outcome by arterialization [Ｊ]. Transplantation, 1993; 56(1)∶19.
- 4. Reck T, Steinbauer F, Steinbauer M, et al. Impact of arterialization on hepatic oxygen supply, tissue energy phosphates, and outcome after liver transplantation in the rat [Ｊ]. Transplantation, 1996; 62(5)∶582.
- 5. Howden B, Jablonski P, Grossman H, et al. The importance of the hepatic artery in rat liver transplantation [Ｊ]. Transplantation, 1989; 47(3)∶428.
- 6. Omura T, Ascher NL, Emond JC. Fiftypercent partial liver transplantation in the rat [Ｊ]. Transplantation, 1996; 62(2)∶292.
- 7. 钱晓峰， 王学浩， 张峰， 等. 大鼠减体积肝移植模型的技术改进及肝再生的初步观察 [Ｊ]. 南京医科大学学报， 2003； 23（3）∶221.
- 8. 孙鹏，蔡端， 张群华，等. 非协调性异种肝移植动物模型的建立： 动脉化模型与静脉化模型的比较 [Ｊ]. 中华肝胆外科杂志， 2001； 7（7）∶442.
- 9. Li J, Dahmen U, Dirsch O, et al. Modified sleeve anastomosis for reconstruction of the hepatic artery in rat liver transplantation [Ｊ]. Microsurgery, 2002; 22(2)∶62.
- 10. Knoop M, Bachmann S, Keck H, et al. Experience with cuff rearterialization in 600 orthotopic liver grafts in the rat [Ｊ]. Am J Surg, 1994; 167(3)∶360.
